Avance 300

Chemicals purchased from commercial sources were used without further purification. All solvents were purified by distillation, dried according to standard procedures or were purchased in HPLC-quality. Preparative (flash) column chromatography was performed on Acros Silica gel 60 (0.035–0.070 mm, 60 Å) as stationary phase. All products were dried in high vacuum (10-3 bar). Thin layer chromatography (TLC) was performed on precoated aluminium silica gel SIL G/UV254 plates (Macherey-Nagel & Co., Düren, NRW, Germany). ¹H-NMR (¹³C-NMR) spectra were recorded at room temperature on a Bruker Avance 300 or 400 or JEOL JNM GX 400 spectrometer. All chemical shifts are given in ppm scale and refer to the non-deuterized proportion of the solvent. NMR raw data was processed with the program MestReNova. Maldi Mass spectra were recorded with Shimadzu Biotech AXIMA Confidence. ESI Mass spectra were recorded with a Bruker Daltonik maXis 4G, a Bruker Daltonik micrOTOF II focus or an Agilent 6120 Quadrupole LCMS System. HPLC spectra were recorded at room temperature on an Agilent Technologies 1200 Series HPLC system. As a stationary phase, the following columns were utilized: IA, IB, IC, AS, OD. IR spectra were recorded on a Varian IR-660 apparatus. The Absorption is indicated in wave numbers (cm⁻¹). Optical rotations were determined on a PerkinElmer polarimeter, model 341, λ = 546 nm.