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Sel1l ab78298

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Sel1L (ab78298) is a protein that functions as part of the endoplasmic reticulum-associated degradation (ERAD) pathway. It plays a role in recognizing and targeting misfolded proteins for degradation. The product is intended for research use only.

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Lab products found in correlation

P erk, by Cell Signaling Technology (2 mentions) α tubulin, by Santa Cruz Biotechnology (2 mentions) Ha h9658, by Merck Group (2 mentions) Ire1α, by Santa Cruz Biotechnology (2 mentions) Pt183 y185 jnk, by Cell Signaling Technology (2 mentions) Caspase 3, by Cell Signaling Technology (2 mentions) Chemidoc xrs system, by Bio-Rad (2 mentions) Ire1β, by Santa Cruz Biotechnology (2 mentions) Hrd1 nb100 2526, by Novus Biologicals (2 mentions) Hsp90, by Santa Cruz Biotechnology (2 mentions)

Spelling variants of this product

Sel1L (4 citations) Ab78298 (2 citations)

2 protocols using sel1l ab78298

1

Quantitative Western Blot Analysis of ER Stress Signaling

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Preparation of cell and tissue lysates and Western blot were performed as previously described27 (link). IRE1α phosphorylation was measured by a Phos-tag-based Western blot method27 (link)–29 (link). For non-reducing SDS-PAGE, lysates were prepared in 5 X non-denaturing sample buffer (250 mM Tris HCl pH 6.8, 1% SDS, 50% glycerol and 0.05% bromophenyl blue) without boiling prior to be separated on a SDS-PAGE gel. Antibodies used in this study were: HSP90 (sc-7947, 1:6,000), GFP (sc-8334, 1:2000), BiP (sc-1051, 1:1,000), IRE1β (sc-20575, 1:1000), JNK1 (sc-571, 1:1000), α-Tubulin (sc-5286, 1:2000) from Santa Cruz; IRE1α (#3294, 1:2000), PERK (#3192, 1:2,000), Caspase-3 (#9665, 1:1000), p-T183/Y185-JNK (#9255, 1:2000), from Cell Signaling; Sel1L (ab78298, 1:2,000), OS9 (ab109510, 1:10,000) from Abcam; Hrd1 (NB100-2526, 1:8,000) from Novus Biologicals; Calnexin (SPA-860, 1:8,000) and PDIA1 (SPA-890, 1:8,000) from Enzo Life Sciences; Flag-HRP (A-8592, 1:8,000), HA (H9658, 1:5,000) from Sigma. Antibodies for Bag6 (rabbit, 1:10,000) and H2A (rabbit, 1:10,000) were kind gifts from Dr. Yihong Ye (NIDDK). Band density was quantitated using the Image Lab software on the ChemiDOC XRS+ system (Bio-Rad). Protein levels were normalized to HSP90 or α-Tubulin and are presented as mean ± s.e.m. unless otherwise specified.
Sun S., Shi G., Sha H., Ji Y., Han X., Shu X., Ma H., Inoue T., Gao B., Kim H., Bu P., Guber R.D., Shen X., Lee A.H., Iwawaki T., Paton A.W., Paton J.C., Fang D., Tsai B., Yates JR I.I.I., Wu H., Kersten S., Long Q., Duhamel G.E., Simpson K.W, & Qi L. (2015). IRE1α is an endogenous substrate of endoplasmic reticulum-associated degradation. Nature cell biology, 17(12), 1546-1555.
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2

Quantitative Western Blot Analysis of ER Stress Signaling

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Preparation of cell and tissue lysates and Western blot were performed as previously described27 (link). IRE1α phosphorylation was measured by a Phos-tag-based Western blot method27 (link)–29 (link). For non-reducing SDS-PAGE, lysates were prepared in 5 X non-denaturing sample buffer (250 mM Tris HCl pH 6.8, 1% SDS, 50% glycerol and 0.05% bromophenyl blue) without boiling prior to be separated on a SDS-PAGE gel. Antibodies used in this study were: HSP90 (sc-7947, 1:6,000), GFP (sc-8334, 1:2000), BiP (sc-1051, 1:1,000), IRE1β (sc-20575, 1:1000), JNK1 (sc-571, 1:1000), α-Tubulin (sc-5286, 1:2000) from Santa Cruz; IRE1α (#3294, 1:2000), PERK (#3192, 1:2,000), Caspase-3 (#9665, 1:1000), p-T183/Y185-JNK (#9255, 1:2000), from Cell Signaling; Sel1L (ab78298, 1:2,000), OS9 (ab109510, 1:10,000) from Abcam; Hrd1 (NB100-2526, 1:8,000) from Novus Biologicals; Calnexin (SPA-860, 1:8,000) and PDIA1 (SPA-890, 1:8,000) from Enzo Life Sciences; Flag-HRP (A-8592, 1:8,000), HA (H9658, 1:5,000) from Sigma. Antibodies for Bag6 (rabbit, 1:10,000) and H2A (rabbit, 1:10,000) were kind gifts from Dr. Yihong Ye (NIDDK). Band density was quantitated using the Image Lab software on the ChemiDOC XRS+ system (Bio-Rad). Protein levels were normalized to HSP90 or α-Tubulin and are presented as mean ± s.e.m. unless otherwise specified.
Sun S., Shi G., Sha H., Ji Y., Han X., Shu X., Ma H., Inoue T., Gao B., Kim H., Bu P., Guber R.D., Shen X., Lee A.H., Iwawaki T., Paton A.W., Paton J.C., Fang D., Tsai B., Yates JR I.I.I., Wu H., Kersten S., Long Q., Duhamel G.E., Simpson K.W, & Qi L. (2015). IRE1α is an endogenous substrate of endoplasmic reticulum-associated degradation. Nature cell biology, 17(12), 1546-1555.
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