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Wps 3000tbrs

Manufactured by Thermo Fisher Scientific
Sourced in United States

The WPS-3000TBRS is a lab equipment product from Thermo Fisher Scientific. It is a temperature-controlled water bath system designed for precise temperature control and monitoring in laboratory applications. The core function of this product is to provide a controlled temperature environment for various experimental and testing procedures.

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3 protocols using wps 3000tbrs

1

Paracetamol Quantification in SODF

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For each formulation, three individual SODFs were dissolved in 100 mL of distilled water. Samples of the solutions were then diluted and the drug concentration was determined by ultra-high performance liquid chromatography (UHPLC, Thermofisher Scientific, Waltham, MA, USA) using a UHPLC-DAD system. It consisted of a Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS equipped with a WPS-3000TBRS autosampler and a TCC-3000RS column compartment set at 35 °C. The system was operated using Chromeleon 7 software (Thermofisher Scientific, Waltham, MA, USA). An Accucore C18 column (2.6 µm, 100 × 2.1 mm2) combined with a security guard ultra-cartridge (Phenomenex Inc., Torrance, CA, USA) was used. An isocratic binary solvent system was utilized, consisting of water/formic acid (1%, v/v) as solvent A and acetonitrile/formic acid (1%, v/v) as solvent B (90%A, 10%B). The flow rate of the mobile phase was 1.5 mL/minute, and the injection volume was 50 μL. Quantitative analysis of paracetamol in the SODFs was carried out using an external standard method. The calibration curve was constructed using 5 different standard levels in the concentration range 1–20 mg/L. The peak of paracetamol was monitored at 244 nm.
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2

Quantification of Ibuprofen Formulations by UHPLC

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For each formulation, three SOFs were dissolved in 100 mL of a mixture (40%/60%) of solvent A (distilled water/formic acid (1%, v/v)) and solvent B (acetonitrile). Samples of the solutions were then diluted and the concentration of the drug was determined by ultra-high performance liquid chromatography (UHPLC) using a UHPLC-DAD system. This consisted of a Thermo Scientific™ Dionex™ UltiMate™ 3000 BioRS equipped with a WPS-3000TBRS autosampler and a TCC-3000RS column compartment set at 35 °C (Thermofisher Scientific, Waltham MA, USA). The system was operated using Chromeleon 7 software. An Accucore C18 column (2.6 µm, 100 mm × 2.1 mm) combined with a security guard ultra-cartridge (Phenomenex Inc., Torrance CA, USA) was used. An isocratic binary solvent system was utilized, consisting of solvent A and solvent B (40%A, 60%B). The flow rate of the mobile phase was 1.5 mL/minute, and the injection volume was 50 μL. Quantitative analysis of IbuAc and IbuNa in the SOFs was carried out using an external standard method. The calibration curve for each form of the drug was constructed using 5 different standard levels of the corresponding form in the concentration range 1–20 mg/L. The peak of ibuprofen was monitored at 258 nm.
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3

Dopamine Quantification in Microdissected Brain Regions

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HPLC on microdissected dopaminergic brain regions was carried out to assess whether tissular DA levels differed between RGS12-null and wild-type mice. Dorsal and ventral striatal brain regions were rapidly dissected and frozen on dry ice. Tissue was then sonicated in 10 volumes of 0.3 N perchloric acid and homogenates were centrifuged at 12,000×g for 15 min. Supernatants were collected, passed through a 0.22 μm filter, and dopamine levels in the homogenates were determined by HPLC with dual-cell electrochemical detection. Cleared lysates underwent automated direct injection of 10 μL into a Dionex Ultimate 3000 HPLC system equipped with a pump (ISO-3100BM, Thermo Fisher Scientific, Waltham, Massachusetts, USA) linked to an autosampler (WPS-3000TBRS, Thermo Fisher Scientific, Waltham, MA, USA), ESA HPLC column (MD-150×3.2) and dual coulometric/amperometric electrochemical detectors (ECD-3000RS, Thermo Fisher Scientific, Waltham, Massachusetts, USA) set at −170 mV and +400 mV, respectively. Mobile phase (MD-TM; Thermo Fisher Scientific, Waltham, Massachusetts, USA) was pumped at a flow rate of 0.6 mL/min. Chromatograms were analyzed using Chromeleon software and dopamine peak height (concentration) was determined from a standard curve. The identity of the DA peak in the sample was confirmed by standard addition.
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