Tecnai 12

Manufactured by Ametek

The Tecnai 12 is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of materials. It features a LaB6 electron source, advanced optics, and a range of analytical capabilities. The Tecnai 12 is well-suited for applications in materials science, nanotechnology, and other fields requiring detailed structural and compositional information at the nanometer scale.

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Lab products found in correlation

Epu software, by Thermo Fisher Scientific (1 mentions) Rio 16 cmos camera, by Ametek (1 mentions) Ccd camera, by Ametek (1 mentions) Tecnai 12, by Thermo Fisher Scientific (1 mentions) Oneview camera, by Ametek (1 mentions)

Spelling variants of this product

Tecnai 12 TEM (14 citations) Tecnai 12 transmission electron microscope (13 citations) Tecnai 12 G2 TEM (5 citations) Tecnai 12 microscope (4 citations) Tecnai 12 Biotwin microscope (3 citations) Tecnai-12 electron microscope (2 citations) Tecnai 12 transmission (2 citations) Tecnai 12 LaB6 electron microscope (2 citations)

3 protocols using tecnai 12

Cryo-EM Imaging of Purified RhopH Complexes

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Purified RhopH protein (4.8 μL of a 0.05 mg/mL solution) was applied to carbon film grids (CF200-Cu, Electron Microscopy) and stained with 4.8 μL of 0.75% uranyl formate for 30 s. After drying, grids were loaded onto a ThermoFischer Tecnai 12 electron microscope with a Gatan Ultra Scan camera operating at 120 kV. Images were collected using EPU software (ThermoFischer) at 67,000× magnification for a pixel size of 1.77 Å. The datasets consisted of between 69 and 142 micrographs (culture-media RhopH, 69 micrographs; complexes containing RhopH2-mV, 109; CLAG3-tv1, 124; CLAG3-GFP, 142).

Schureck M.A., Darling J.E., Merk A., Shao J., Daggupati G., Srinivasan P., Olinares P.D., Rout M.P., Chait B.T., Wollenberg K., Subramaniam S, & Desai S.A. (2021). Malaria parasites use a soluble RhopH complex for erythrocyte invasion and an integral form for nutrient uptake. eLife, 10, e65282.

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Ultrastructural Analysis of Mitochondria in A498 Cells

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Electron microscopic analysis of indicated A498 cells was performed as detailed below. Sample prep: indicated A498 cell culture pellets were embedded in 2% agarose, secondary fixation with osmium tetroxide, dehydration (25,50,70,95100% EtOH), followed by embedding in EPON. Sectioning: ≈80 nm thin sections on Cu grids, stained with UA and LC. Imaging: FEI Tecnai 12 at 120 kV, Gatan Rio16 CMOS camera. Cells were randomly selected for imaging. Images at 4400× magnification were used for counting total and abnormal mitochondria. All clearly identified mitochondria in the images were analyzed. Images at 26 000× magnification were randomly selected for mitochondria‐ER contact (MERC) length measurement. All clearly identified mitochondria membranes and associated ER membranes in the images were evaluated. Only MERC sites with a distance no more than 30 nm were regarded as MERC enabling calcium transfer from ER to mitochondria and their length was measured according to.^[⁸⁰^] Image analysis was performed using Adobe Illustrator.

Zhu Z., Zhou X., Du H., Cloer E.W., Zhang J., Mei L., Wang Y., Tan X., Hepperla A.J., Simon J.M., Cook J.G., Major M.B., Dotti G, & Liu P. (2022). STING Suppresses Mitochondrial VDAC2 to Govern RCC Growth Independent of Innate Immunity. Advanced Science, 10(3), 2203718.

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Electron Microscopy Particle Analysis

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Images of samples and various centrifugation fractions were recorded on a CM120 electron microscope with LaB6 illumination operating at 120 KeV or Tecnai 12 electron microscopy at 100 KeV (FEI, Eindoven, Netherlands). For negative staining, samples were stained with 1% uranyl acetate or 3% phosphotungstic acid. Images were recorded on a 1,024 × 1,024-pixel CCD camera (Gatan, Pleasanton, CA) for CM120 at a nominal magnification of 77,000 ✠ at the CCD (0.316 nm pixels). For the Tecnai 12 electron microscope images were recorded on a 4096 × 4,096-pixel OneView camera (Gatan) at nominal magnifications of 30,000 ✠ (0.377 nm pixels) and 52,000 ✠ (0.217 nm pixels). Samples were negatively stained with uranyl acetate replacement (UAR) (Electron Microscopy Sciences, Hatfield, PA). Diameter measurements of particles were carried out in IMOD (50 (link)) by modeling major and minor elliptical axes with line-segments, and results were reported using the standard deviation to estimate error. Statistical significance between particle diameters was determined using Tukey’s HSD test (to account for multiple pairwise comparisons), as implemented in the statistical software R.

Gallagher J.R., Torian U., McCraw D.M, & Harris A.K. (2017). Characterization of the disassembly and reassembly of the HBV glycoprotein surface antigen, a pliable nanoparticle vaccine platform. Virology, 502, 176-187.

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