Cadmium chloride cdcl2

Artichoke waste was obtained as a byproduct of canned artichoke processing from Huimei Agricultural Technology Co., Ltd. (Changde, China). Alkaline protease (enzyme activity ≥ 200 U/mg), high-temperature- resistant α-amylase (enzyme activity ≥ 40 U/mg) and diastatic enzyme (enzyme activity ≥ 10 U/mg) were obtained from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). All other chemicals were of analytical grade. Basal diet (21% casein, 0% ADF, 7% sunflower oil, 1% cholesterol, 15% sucrose, 51.2% starch, 0.3% methionine, 3.6% minerals, 1% mixed vitamins), ADF special diet (basal diet plus 2% ADF), HPH-ADF special diet (basal diet plus 2% HPH-ADF) and CM-ADF special diet (basal diet plus 2% CM-ADF) were prepared by Jiangsu Medison Biomedical Co. (Jiangsu, China). The kits for measurement of glutathione peroxidase (GSH-PX) were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The kits for measurement of aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine (CREA-S) and urea were obtained from Shenzhen Mindray Bio-medical Electronics Co. Ltd. (Shenzhen, China). Cadmium chloride (CdCl₂) of analytical grade was supplied by Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China).

Fetal bovine serum (FBS), RPMI-1640 medium, trypsin, penicillin, and streptomycin were obtained from Gibco (Waltham, MA, USA). Cadmium chloride (CdCl₂) and other analytical-grade laboratory reagents were obtained from Sinopharm Chemical Reagent Company (Shanghai, China). iTRAQ reagents were purchased from Applied Biosystems (Waltham, MA, USA).
Kits purchased from SenBeiJia Biotechnology (Nanjing, China) were used to determine the contents of nitric oxide (NO), 5-hydroxytryptamine (5-HT), substance P, acetylcholinesterase (AChE), vasoactive intestinal peptide (VIP), and calcitonin gene-related peptide (CGRP). TRIzol reagent was obtained from Life Technologies (Waltham, MA, USA). Reverse transcription was performed using a kit purchased from Takara Bio (Beijing, China).