A blood sample was collected after an overnight fast of 8 h. Plasma glucose levels were measured using COBAS INTEGRA 400 plus (Roche, USA). Fasting insulin levels were measured using a radioimmunoassay by Immulite I (Siemens, USA). Fasting lipids were analyzed, and for the present study serum levels of total cholesterol, LDL, HDL and triglyceride using COBAS INTEGRA 400 plus (Roche, USA).
Cobas integra 400 plus
Manufactured by Roche
Sourced in Switzerland, Germany, United States, Australia, China, United Kingdom, Philippines
The Cobas Integra 400 Plus is a clinical chemistry analyzer designed for in-vitro diagnostics. It is capable of performing a variety of tests on biological samples to aid in the diagnosis and monitoring of medical conditions. The core function of the Cobas Integra 400 Plus is to analyze samples and provide quantitative results for clinical parameters.
Automatically generated - may contain errors
Lab products found in correlation
Cobas 6000, by Roche (9 mentions)
Elecsys 2010, by Roche (5 mentions)
Vacutainer, by BD (5 mentions)
Advia 120, by Siemens (4 mentions)
Au680, by Beckman Coulter (4 mentions)
Cobas e411 analyzer, by Roche (4 mentions)
Cobas e411, by Roche (3 mentions)
Cobas integra, by Roche (3 mentions)
Urilux, by Roche (3 mentions)
Sedivue dx, by IDEXX (3 mentions)
Immulite 1000, by Siemens (3 mentions)
Advia 2120, by Siemens (3 mentions)
Kx 21n, by Sysmex (3 mentions)
Sapphire 2, by Tecan (2 mentions)
Elecsys insulin test, by Roche (2 mentions)
Eia 3954, by DRG International (2 mentions)
Enzyme linked immunosorbent assay kit, by BD (2 mentions)
Facscalibur, by BD (2 mentions)
Cobas 6000 analyzer, by Roche (2 mentions)
Cobas 411, by Roche (2 mentions)
266 protocols using cobas integra 400 plus
1
Fasting Metabolic Biomarker Measurements
2
Comprehensive Diagnostic Evaluation of CKCD
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Whole blood samples in potassium‐EDTA tubes for CBC (Advia 120 or 2120i, Siemens, Medical Solutions Diagnostics GmbH, Erlangen,, Germany; Abacus Junior Vet, Diatron, Wien, Austria) and whole blood samples in plain tubes with gel separators for serum biochemistry (Cobas Integra 400 Plus or Cobas 6000, Roche, Mannheim, Germany) were collected at presentation and analyzed within 60 minutes after collection. Urine samples were obtained by cystocentesis for urinalysis including dipstick chemistry (Urilux, Roche, Mannheim Germany) and sediment cytology (SediVue Dx, IDEXX Laboratories Inc, Westbrook, Maine or manual microscopy), as well as for bacterial culture. Urine specific gravity (USG) was measured using a clinical refractometer (Atago, Tokyo, Japan). Pyuria and hematuria were defined as presence of >5 leukocytes or erythrocytes, respectively per high‐power field. Bacteriuria was diagnosed if bacteria were observed in sediment cytology. Proteinuria was defined as a urine dipstick result of ≥1+ (ie, ≥30 mg/dL). Urine protein‐to‐creatinine ratio (Cobas Integra 400 Plus or Cobas 6000, Roche, Mannheim, Germany) was only measured in dogs with severe proteinuria (urine dipstick result +4) and clinical concern that glomerular disease was the inciting cause for ACKD.
3
Metabolic Profile Assessment in T2DM Patients
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Venous fasting blood samples were collected from each participant into tubes containing EDTA (ethylene diamine tetraacetic acid), fluoride oxalate and gel separator. Fasting plasma glucose (FPG) in fluoride tubes and glycated haemoglobin (HbA1c) in EDTA tubes were measured on an automated chemistry analyser (Roche Diagnostics, COBAS INTEGRA 400 Plus, USA). Similarly, serum total cholesterol (TC), high density lipoprotein cholesterol (HDL-c), low density lipoprotein cholesterol (LDL-c), and triglycerides (TG) were measured on the automated chemistry analyser (Roche Diagnostics, COBAS INTEGRA 400 Plus, USA). Non-HDL was calculated as Non-HDL = total cholesterol-HDL. Coronary risk ratio and very low density lipoprotein (VLDL) cholesterol were calculated on the automated chemistry analyser. Various medications utilised by the T2DM patients at the clinic are shown in Fig. 1 .![]()
Category of medications utilised by T2DM patients
4
Measuring Urinary Albumin-Creatinine Ratio
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Urinary albumin and creatinine were measured on a morning urine sample using an automatic analyser (COBAS INTEGRA 400 plus, Roche, Basel, Switzerland). Creatinine was measured by Jaffe's kinetic method, and albuminuria was measured by immunoturbidimetric methods. Urinary albumin-to-creatinine ratio (ACR, milligram per gram) was calculated. The term of albuminuria was used to describe the increase in ACR of 30 mg/g or over. Serum creatinine (Scr) and uric acid were measured by the autoanalyser (COBAS INTEGRA 400 plus, Roche, Basel, Switzerland). Estimated glomerular filtration rate (eGFR) was calculated using the following estimating equation which was developed by modifying the Modification of Diet in Renal Disease (MDRD) equation based on the data from Chinese CKD patients [16] (link), eGFR (mL/min/1.73 m2) = 175×Scr (mg/dL)−1.234×age(year)−0.179 [female×0.79].
5
Comprehensive Metabolic Panel Analysis
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Blood urea nitrogen, creatinine, albumin, total protein, total cholesterol, triglyceride, calcium, phosphate, iron, hematocrit and hemoglobin were measured using standard methods. High density lipoprotein cholesterol (HDL-L) was measured by homogeneous enzymatic colorimetric assay (COBAS INTEGRA ® 400 plus, Roche), parathyroid hormone by immunoassay (COBAS e 411 analyzer-Roche) and C-reactive protein (CRP) by particle enhanced turbidimetric assay (COBAS INTEGRA ® 400 plus, Roche). All samples for a given assay were tested simultaneously, in duplicate and in appropriate dilutions, according to conventional protocols.
6
Comprehensive Blood and Metabolic Panel
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Cell blood count (CBC) on Sysmex KX-21, (Sysmex, Kobe, Japan) and SGOT, SGPT, total protein and albumin (liver function tests on Cobas Integra 400 plus [Roche, Penzberg, Germany] and urea and creatinine (kidney function tests on Cobas Integra 400 plus [Roche, Penzberg, Germany], and serum Cu and Zn levels by direct colorimetric method.
7
Metabolic Biomarkers Measurement Protocol
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A blood sample was collected at 0700 h after an overnight fast of 8 h. Plasma glucose levels and Hb1Ac were measured using COBAS INTEGRA 400 plus (Roche®, Indianapolis, IN, USA). Cortisol levels were measured using a radioimmunoassay by Tri-carb 2100 tr Liquid Scintillation Analyzer (Packard®, Conroe, TX, USA). Serum samples for fasting lipids were analyzed, and for the present study, serum levels of total cholesterol, LDL, HDL, and triglycerides were measured as well using COBAS INTEGRA 400 plus (Roche®, Indianapolis, IN, USA).
8
Comprehensive Blood Biomarker Analysis
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Fasting blood samples were obtained between 8 and 10 a.m. All laboratory parameters were measured at the accredited laboratory of Tartu University Hospital using standard methods. A turbidimetric immunoassay was used to measure hsCRP (Cobas 501, Roche Diagnostics GmbH, Germany), ceruloplasmin (Cobas Integra 400 Plus, Roche Diagnostics GmbH, Germany), soluble transferrin receptors (Cobas Integra 400 Plus, Roche Diagnostics GmbH, Germany), transferrin (Cobas 501, Roche Diagnostics GmbH, Germany), and transferrin saturation (Cobas 501, Roche Diagnostics GmbH, Germany). The enzymatic colorimetric method was used to measure uric acid (Cobas 501, Roche Diagnostics GmbH, Germany), and the electrochemiluminescence assay (ECLIA) was used to measure ferritin (Cobas e601, Roche Diagnostics GmbH, Germany) and IL-6 (Cobas e402, Roche Diagnostics GmbH, Germany). The complete blood count was analysed with a Sysmex XN-9000/XN-9100 analyser (Sysmex Corporation, Kobe, Japan).
9
Comprehensive Serum and Urine Analysis
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We analysed the serum samples with the Cobas Integra 400 plus (Roche Basel, Switzerland), and glucose, total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-c), high-density lipoprotein cholesterol (HDL-c), γ-glutamyl transferase (GGT) and creatinine levels were determined. Serum C-reactive protein (CRP) levels were determined by means of a particle-enhanced turbidimetric assay. Glycosylated haemoglobin (HbA1c) was determined using the D-10 Haemoglobin testing system (Bio-Rad, #220-0101) by means of ion-exchange high-performance liquid chromatography.
We also analysed the urinary creatinine and albumin levels using the Cobas Integra 400 plus (Roche, Basel, Switzerland) by means of a kinetic colorimetric assay.
The inter- and intra-assay coefficients of variability for the biochemical analyses were as follows: glucose (1.8%; 2.1%), TC (0.2%; 1.9%), HDL-c (1.1%; 1.0%), LDL-c (1.5%; 1.9%), GGT (1.8%; 1.8%), CRP (1.3%; 3.5%), HbA1c (0.9%; 1.3%), creatinine (1.4%, 2.5%) and albumin (1.9%; 2.2%).
We also analysed the urinary creatinine and albumin levels using the Cobas Integra 400 plus (Roche, Basel, Switzerland) by means of a kinetic colorimetric assay.
The inter- and intra-assay coefficients of variability for the biochemical analyses were as follows: glucose (1.8%; 2.1%), TC (0.2%; 1.9%), HDL-c (1.1%; 1.0%), LDL-c (1.5%; 1.9%), GGT (1.8%; 1.8%), CRP (1.3%; 3.5%), HbA1c (0.9%; 1.3%), creatinine (1.4%, 2.5%) and albumin (1.9%; 2.2%).
10
Urinary Creatinine, Uric Acid, and Purine Metabolites in Sheep
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Urinary creatinine was analysed using a Cobas Integra 400 plus (Basel, Roche, Switzerland), and urinary uric acid and allantoins were determined using the uricase method and colorimetric method, respectively [20 ]. Purine derivatives excretion was estimated using the equation by [21 (link)].
Blood samples were collected from two randomly selected sheep per replication group on measurement days 17 and 25. One 10 ml blood sample per sheep was collected from the jugular vein into a lithium heparin vacuette tube (BD 367526) using a 18G vacutainer needle (VACU NS2) and syringe (SYRI HS10). The blood was then centrifuged at 3500 rpm at 4 °C for 10 min to harvest plasma. Plasma urea N (PUN) and plasma glucose were analysed using a Cobas Integra 400 plus (Roche, Switzerland). Urinary N was predicted via the equation: Urinary N, g/d = 1.2 (L/d/kg LW) × PUN (g/L) × LW (kg) [22 (link)].
Blood samples were collected from two randomly selected sheep per replication group on measurement days 17 and 25. One 10 ml blood sample per sheep was collected from the jugular vein into a lithium heparin vacuette tube (BD 367526) using a 18G vacutainer needle (VACU NS2) and syringe (SYRI HS10). The blood was then centrifuged at 3500 rpm at 4 °C for 10 min to harvest plasma. Plasma urea N (PUN) and plasma glucose were analysed using a Cobas Integra 400 plus (Roche, Switzerland). Urinary N was predicted via the equation: Urinary N, g/d = 1.2 (L/d/kg LW) × PUN (g/L) × LW (kg) [22 (link)].
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