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Bptes

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BPTES is a laboratory reagent produced by MedChemExpress. It functions as a selective inhibitor of glutaminase, an enzyme involved in cellular metabolism. BPTES can be utilized in research applications to study glutamine-dependent pathways and their regulation.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) H2dcfda, by Thermo Fisher Scientific (2 mentions) Gsh assay kit, by Solarbio (1 mentions) 2 7 dichlorofluorescein diacetate dcfh da, by Merck Group (1 mentions) Human serum albumin (hsa), by Merck Group (1 mentions) Hoechst 33342, by Solarbio (1 mentions) 2 deoxy d glucose 2 dg, by MedChemExpress (1 mentions) Etomoxir, by MedChemExpress (1 mentions) 5 fluorouracil 5 fu, by MedChemExpress (1 mentions) Monobromobimane, by Thermo Fisher Scientific (1 mentions) Crystal violet, by Thermo Fisher Scientific (1 mentions) Mitosox red, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions) Lenvatinib, by Selleck Chemicals (1 mentions) Sorafenib, by MedChemExpress (1 mentions) Trolox, by Selleck Chemicals (1 mentions) β actin sc 47778, by Santa Cruz Biotechnology (1 mentions) Triton x 100, by Avantor (1 mentions) Automated microplate reader, by Tecan (1 mentions)

4 protocols using bptes

1

Photodynamic Therapy Protocol for Cancer

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BPTES was purchased from MedChemExpress LLC (Shanghai, China). Ce6 was purchased from J&K Scientific Ltd (Beijing, China). HSA and 2′,7′-dichlorofluorescein diacetate (DCFH-DA) were obtained from Sigma-Aldrich (USA). 1,3-diphenylisobenzofuran (DPBF) and 3-(4,5-dimethylthia-zol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were ordered from Aladdin Industrial Corporation (Shanghai, China). Hoechst 33,342 and GSH assay kit were ordered from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). Roswell Park Memorial Institute (RPMI) 1640 medium, trypsin and penicillin-streptomycin solution were obtained from Meilun biology co., Ltd (Dalian, China). Fetal bovine serum (FBS) was purchased Tianhang Biotechnology Co., Ltd (Ningbo, China).
Yu C., Wang N., Chen X., Jiang Y., Luan Y., Qin W, & He W. (2023). A photodynamic-mediated glutamine metabolic intervention nanodrug for triple negative breast cancer therapy. Materials Today Bio, 19, 100577.
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2

Comprehensive Metabolic Modulation

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Dimethyl sulfoxide (DMSO), FAO activator bezafibrate (BZF) and inhibitor etomoxir (ETO), glycolysis inhibitor 2-Deoxy-D-glucose (2-DG), glutaminase inhibitor BPTES, P300 inhibitor C646 and 5-fluorouracil (5-FU) were all purchased from MedChemExpress, New Jersey, USA.
Zhang M., Peng R., Wang H., Yang Z., Zhang H., Zhang Y., Wang M., Wang H., Lin J., Zhao Q, & Liu J. (2022). Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells. Cell Death & Disease, 13(2), 159.
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3

Metabolic Regulation in Cancer Cells

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BPTES (HY-12683), CBR-5884 (HY-100012), SHIN1 (HY-112066), DS18561882 (HY-130251), and sorafenib (HY-10201) were purchased from MedChemExpress. Trolox (S3665), lenvatinib (S1164) were from selleckchem. Crystal violet (V5265), H2DCFDA (D399), MitoSOXTM Red (M36008), monobromobimane (mBBr, M1378), Propidium Iodide (P3566), RNase A (10109169001), (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT, M6494), Hoechst 33342 (H3570), and LipofectamineTM RNAiMAX Transfection Reagent (13778150) were obtained from Thermo Fisher Scientific. H2O2 (216763) was purchased from Sigma-Aldrich. Triton X-100 (0694) was purchased from AMRESCO. Primary antibodies against SHMT2 (sc-390641), PHGDH (sc-100317), and β-actin (sc-47778) were purchased from Santa Cruz Biotechnology. MTHFD2 (98116 S) was acquired from Cell Signaling Technology. Secondary antibodies of the mouse and rabbit were from Cell Signaling Technology and Abcam respectively (7076 S and ab6721). Small interfering RNA (siCTRL and siATF4) oligonucleotides were purchased from Santa Cruz Biotechnology (sc-37007 and sc-35112, respectively).
Hwang Y., Yun H.J., Jeong J.W., Kim M., Joo S., Lee H.K., Chang H.S., Kim S.M, & Fang S. (2023). Co-inhibition of glutaminolysis and one-carbon metabolism promotes ROS accumulation leading to enhancement of chemotherapeutic efficacy in anaplastic thyroid cancer. Cell Death & Disease, 14(8), 515.
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4

NF-κB and Glutaminase Inhibitor Cytotoxicity

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Macrophages (1 × 105 cells/well) were exposed to various concentrations of an NF-κB signaling pathway inhibitor BAY (BAY 11-7082, # HY-13453) or glutaminase (Gls) inhibitor BPTES (MedChemExpress, New Jersey, USA # HY-12683) in DMEM. The medium was replaced with fresh DMEM after 24 h culture. CCK-8 reagent (10 μL/well) (Beytotime, Jiangsu, China) was then added to the culture medium, followed by incubation for 4 h. The optical density at 450 nm was read on an automated microplate reader (Tecan Sunrise, CH).
Zhao T., Zhang Z., Li Y., Sun Z., Liu L., Deng X., Guo J., Zhu D., Cao S., Chai Y., Nikolaevna U.V., Maratbek S., Wang Z, & Zhang H. (2023). Brucella abortus modulates macrophage polarization and inflammatory response by targeting glutaminases through the NF-κB signaling pathway. Frontiers in Immunology, 14, 1180837.
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