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Anti human foxp3 staining kit

Manufactured by BD
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The Anti-Human FOXP3 Staining Kit is a laboratory reagent used for the detection and quantification of FOXP3, a transcription factor that is primarily expressed in regulatory T cells. The kit provides the necessary components, including antibodies and buffers, to perform intracellular staining and flow cytometric analysis of FOXP3-expressing cells.

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Lab products found in correlation

Accuri c6 flow cytometer, by BD (1 mentions) Anti cd4 antibody, by BD (1 mentions) Accuri c6, by BD (1 mentions)

Close spelling variants of this product

Human FOXP3 Staining Kit Foxp3 staining kit Anti-Foxp3

2 protocols using anti human foxp3 staining kit

1

Quantifying Regulatory T Cells

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CD4+ T cells were stained with Anti-Human FOXP3 Staining Kit (560133; BD Biosciences), and the percentage of CD4+CD25+FOXP3+ cells in CD4+ T cell population was analyzed on Accuri C6 flow cytometer (BD Biosciences) with FlowJo 7.6.1 software (Tree Star Inc.).
Wang L., Wang L., Hao P., Cao Q, & Zhang Z. (2019). Anti-CCL22 increases regulatory T cells in CD4+ T cells of rheumatoid arthritis patients via STAT5 pathway. Experimental and Therapeutic Medicine, 19(3), 2127-2132.
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2

Quantification of Human Regulatory T Cells

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CD4+ T cells isolated from PBMCs were stained with either isotype control antibody (cat. no. 553452 for anti-Foxp3 and anti-CD25 antibody; cat. no. 555909 for anti-CD4 antibody, all from BD Biosciences; Becton, Dickinson and Company) or anti-human CD4, anti-human CD25 and anti-Human Foxp3 antibodies, according to the protocol of the Anti-Human Foxp3 Staining kit (cat. no. 560133; BD Biosciences; Becton, Dickinson and Company). The proportion of CD4+CD25+Foxp3+ Treg cells was analyzed using a flow cytometer (BD Accuri C6, software version 1.0.264.21; BD Biosciences; Becton, Dickinson and Company).
Wang R, & Huang K. (2020). CCL11 increases the proportion of CD4+CD25+Foxp3+ Treg cells and the production of IL-2 and TGF-β by CD4+ T cells via the STAT5 signaling pathway. Molecular Medicine Reports, 21(6), 2522-2532.
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