The fluorescence spectra were recorded on a Hitachi F-7100 spectrophotometer. The absorption spectra were recorded on a Shimadzu UV-3101PC spectrophotometer. A stock solution of NAF (0.017 mg ml−1) was prepared in acetonitrile–water. Colistin stock solutions (0.21 mg ml−1) and LPS stock solutions (1.718 mg ml−1) of different concentrations were prepared in Millipore water. The experiments were carried out in acetonitrile : water (2 : 5, v/v), buffered with 1 mM phosphate buffer (pH 7.0). During the titration, each time 35 μl of a 0.017 mg per ml solution of NAF was placed in a Starna quartz optical cell (sub-micro) of 3 mm optical path length, z = 15 mm, and then colistin and LPS solutions were added incrementally into the quartz optical cell using a micropipette. For a simultaneous discharge, premixed LPS–colistin solution was gradually added and titrated against NAF. The spectral data were recorded immediately after the addition of the colistin–LPS solution.
F 7100 spectrophotometer
Manufactured by Hitachi
Sourced in Japan
The F-7100 spectrophotometer is a laboratory instrument manufactured by Hitachi. It is designed to measure the absorbance or transmittance of light through a sample across a range of wavelengths. The core function of the F-7100 is to quantify the interaction between light and a sample material, providing data that can be used for various analytical and research applications.
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5 protocols using f 7100 spectrophotometer
1
Fluorescence Titration of NAF with Colistin and LPS
2
Spectroscopic Characterization of LH2 Proteins
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Electronic absorption and CD spectra of LH2 proteins were measured with a Shimadzu UV-2450 spectrophotometer (Shimadzu, Kyoto, Japan) and a JASCO J-820 spectropolarimeter (JASCO, Tokyo, Japan). Fluorescence emission spectra were measured with a Hitachi F-7100 spectrophotometer (Hitachi, Tokyo, Japan). The apparent fluorescence quantum yields of B850 BChl a were measured by a Hamamatsu C9920-03G fluorescence measurement system (Hamamatsu Photonics, Shizuoka, Japan) from emission between 840 and 950 nm.
3
Optical Properties of 1Poly in Aqueous MeOH Solution
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The optical properties of 1poly were evaluated in an aqueous MeOH solution (MeOH : water = 3 : 1, v/v) containing MES (12.5 mM) and NaCl (12.5 mM) of pH 5.5 at 25 °C. UV-vis absorption spectra of 1poly (4.0 × 10−4 M per unit) were recorded using a Shimadzu UV-2600 spectrophotometer within the wavelength range 300 to 650 nm. A Hitachi F-7100 spectrophotometer was applied to record the fluorescence emission spectra of 1poly (4.0 × 10−5 M per unit) from 450 to 750 nm at a scan rate of 240 nm min−1. The slit widths for excitation and emission were set to 20 and 10 nm, respectively. The excitation wavelength for metal ion detection was selected as 425 nm according to an isosbestic point observed in the UV-vis titrations of 1poly for metal ions.
4
Comprehensive Characterization of Novel Materials
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Ultraviolet absorption spectra were recorded using a Shimadzu UV-2550 and an F-7100 spectrophotometer (Hitachi, Japan) was employed to collect fluorescence emission spectra. The morphologies of samples were observed by scanning electron microscopy (SEM, Hitachi S4800, Japan) and transmission electron microscopy (TEM) with a JEM-1400 microscopy system (JEOL, Japan) at an acceleration voltage of 120 kV. AFM images were acquired using a Cypher S atomic force microscope (AFM, Oxford instruments, Britain) in contact mode. X-ray diffraction (XRD) spectra were recorded using a Rigaku Ultima IV instrument (Kuraray, Japan) with a Cu target (λ = 1.54051 Å) operated at 40 kV and 15 mA. The ECL response was recorded with a model MPI-A ECL analyzer from Xi'an Remax Electronic Science & Technology Co. Ltd (Xi'an, China) combined with a CHI 660C electrochemistry workstation from Shanghai CH Instruments (Shanghai, China) to perform electrochemical measurements simultaneously. The photomultiplier tube (PMT) was biased at 600 V. A conventional three-electrode system was introduced in the process, which comprised a modified GCE (Φ = 3 mm) as the working electrode, Ag/AgCl (saturated KCl solution) as the reference electrode, and a platinum wire as the counter electrode.
5
Doxorubicin Fluorescence Emission Spectra
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Fluorescence emission spectra of doxorubicin hydrochloride (DOX) were measured on a Hitachi F-7100 spectrophotometer (Hitachi, Japan) at 25 °C with an excitation wavelength of 481 nm. Both excitation and emission slit widths were kept at 10 nm.
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