Stereotactic injections of lentivirus were performed in 8‐week‐old C57BL/6J mice. Prior to stereotactic injections and on the day following the surgery, mice were treated with analgesics (Rimadyl®, Zoetis, 10 mg/kg of body weight in 0.9% NaCl) by subcutaneous injection and then anesthetized with a combination of 0.5 mg/kg Medetomidine (Pfizer), 5 mg/kg Midazolam (Hameln), and 0.05 mg/kg Fentanyl (Albrecht) (in 0.9% NaCl) by intraperitoneal injection. Mice were placed in a stereotactic frame and kept on an animal heating pad to control body temperature during surgery. A small craniotomy was performed and 1 μl of the lentivirus was slowly injected using a pulled glass capillary (Hirschmann, 9600105) into the dentate gyrus at the following coordinates relative to Bregma: −2.0 AP (anterior–posterior), 1.4 ML (medio‐lateral), −2.0 DV (dorso‐ventral). The capillary was left in place for another 5 min before withdrawal to allow diffusion of the virus. Then, anesthesia was antagonized by intraperitoneal injection of 2.5 mg/kg Atipamezol (Pfizer), 0.5 mg/kg Flumazenil (Hameln), and 0.1 mg/kg Buprenorphine (RB Pharmaceuticals) (in 0.9% NaCl). Animals were allowed to recover, returned to their home cages, and their physical condition was monitored daily. Rimadyl®, Zoetis 15 mg/kg was administered via drinking water for 2 days after surgery.
Rimadyl
Manufactured by Zoetis
Sourced in United States, Germany, Japan, United Kingdom, Italy, Greece, Spain
Rimadyl is a non-steroidal anti-inflammatory drug (NSAID) for veterinary use. It is designed to reduce inflammation, pain, and fever in dogs.
Automatically generated - may contain errors
Lab products found in correlation
Rompun, by Bayer (7 mentions)
Carprofen, by Zoetis (7 mentions)
Particulate, by Polysciences (4 mentions)
Baytril, by Bayer (4 mentions)
Ketaset, by Zoetis (2 mentions)
Synulox rtu inj 100 ml, by Zoetis (2 mentions)
Buprenex, by Reckitt Benckiser (2 mentions)
Isoflo, by Zoetis (2 mentions)
Temgesic, by Reckitt Benckiser (2 mentions)
Propofol 1 mct lct, by Fresenius (2 mentions)
Isoflo, by Abbott (2 mentions)
Dolorex, by MSD (2 mentions)
C57bl 6j, by Jackson ImmunoResearch (2 mentions)
Isoflurane anesthesia, by Performance Health (2 mentions)
Stereotactic frame, by Kopf Instruments (1 mentions)
Ketamine, by Kopf Instruments (1 mentions)
Xylazine, by Kopf Instruments (1 mentions)
Adrenalin, by AstraZeneca (1 mentions)
Xylocain, by AstraZeneca (1 mentions)
Greenline gm500, by Tecniplast (1 mentions)
88 protocols using rimadyl
1
Stereotactic Lentiviral Injections in Mice
2
Postoperative Recovery and Wound Healing in Dogs
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During recovery, dogs were observed in the recovery room until they were discharged on the same day as the surgery had been performed. At 3 h after extubation, 2.2 mg/kg carprofen (Rimadyl, Zoetis, Lincoln, USA) was administered subcutaneously. All dogs were treated with cephalexin, 25 mg/kg, (Lexporin, Nida Pharma, Ayutthaya, Thailand) and carprofen, 2.2 mg/kg, (Rimadyl, Zoetis, Lincoln, USA) orally twice daily for 7 days. Owners were telephone-interviewed on one occasion about their dog’s recovery status 3–5 days after surgery. Seven to fifteen days after surgery, dogs were recalled for evaluation of the wound healing and removal of skin sutures.
3
BTPC Intracranial Injection Protocol in NMRI Nude Mice
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For BTPC intracranial injections, 6- to 10-week-old NMRI nude mice were used. Tumor cells were harvested by treatment with Accutase and 200,000 - 1,000,000 cells per mouse were re-suspended in 4 μl of Neurobasal-A medium naked and stored on ice until injection with a 10 μl Hamilton syringe.
30 min before surgery, experimental mice were subcutaneously injected with 400 μl of Carprofen (Rimadyl®, 4 mg/kg, Zoetis, Madison, New Jersey, USA) solution to decrease pain perception throughout and 24 h after surgery. Mice were anesthetized with intraperitoneal injection of 120 μl per 10 g body weight ketamine/xylazine mixture (40 mg/kg, 5 mg/kg) (ketamine - Ratiopharm, Ulm, Germany; xylazine - Bayer Vital, Leverkusen, Germany) and placed into the stereotactic frame (Kopf Instruments, Tujunga, California, USA). The scalp was disinfected, cut open, a burr hole drilled 2 mm lateral to the bregma into the left hemisphere and the needle introduced to a depth of 3 mm. 2 μl of cell suspension was injected at a flow rate of 400 nl/min. The injected cells were allowed to settle for 5 min before the needle was removed and the wound sewn.
30 min before surgery, experimental mice were subcutaneously injected with 400 μl of Carprofen (Rimadyl®, 4 mg/kg, Zoetis, Madison, New Jersey, USA) solution to decrease pain perception throughout and 24 h after surgery. Mice were anesthetized with intraperitoneal injection of 120 μl per 10 g body weight ketamine/xylazine mixture (40 mg/kg, 5 mg/kg) (ketamine - Ratiopharm, Ulm, Germany; xylazine - Bayer Vital, Leverkusen, Germany) and placed into the stereotactic frame (Kopf Instruments, Tujunga, California, USA). The scalp was disinfected, cut open, a burr hole drilled 2 mm lateral to the bregma into the left hemisphere and the needle introduced to a depth of 3 mm. 2 μl of cell suspension was injected at a flow rate of 400 nl/min. The injected cells were allowed to settle for 5 min before the needle was removed and the wound sewn.
4
Neuropharmacological Intervention in Rats
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The surgery was conducted as earlier described,25 where two guides (stainless steel, length 10 mm, with an o.d./i.d. of 0.6/0.45 mm) targeting the NAc shell were implanted 2 days prior to the drug challenge. During surgery, the rats were kept on a heating pad to prevent hypothermia. The rats were anaesthetized with isoflurane (Isofluran Baxter, Kronans Apotek) and a local anaesthetic mixture [Xylocain (10 mg/ml) together with adrenalin (5 μg/ml), Astra Zeneca, Kronans Apotek] was applied on the skull surface. After incision, the skull bone was exposed, and three holes were drilled: two for the guides35 (Table S2 ) and one for the anchoring screw. The tips of the guides were inserted 1 mm below the skull bone. The guides were anchored to the screw and the skull bone with dental cement (DENTALON®plus; Agntho's AB, Lidingö, Sweden). Carprofen (5 mg/kg, SC, Rimadyl® ; Zoetis, Kronans Apotek) was used to relieve pain following surgery.
At the experimental day, a dummy cannula was carefully inserted and retracted into the guide to remove clotted blood and hamper spreading depression within NAc shell. One hour later, a cannula delivered the drug into the NAc shell (TableS2 ), the drug was delivered over 1 min, and after an additional minute, the cannula was retracted.
At the experimental day, a dummy cannula was carefully inserted and retracted into the guide to remove clotted blood and hamper spreading depression within NAc shell. One hour later, a cannula delivered the drug into the NAc shell (Table
5
Synthetic and Biological Scaffolds for Muscle Defect Repair
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Animal procedures were performed in adherence to approved JHU IACUC protocols. Surgeries were performed when animals were 10 weeks of age. The bilateral traumatic muscle defect was created as previously described20 (link). The defects were filled with 30 mg of a synthetic material or biological scaffold material. PCL was employed as a synthetic material (particulate, Mn = 50,000 g/mol, mean particle size < 600 μm, Polysciences). In turn, as a biological scaffold material, decellularized urinary bladder matrix (Matristem, Acell) was implanted from 0.05 ml of a 400 mg/ml suspension in phosphate buffered saline (PBS). Control surgeries were injected with 0.05 ml of PBS as a no implant control. All materials were UV sterilized prior to use. Mice were given subcutaneous carprofen (Rimadyl, Zoetis) at 5 mg/kg for pain relief. For the sample harvest, mice were euthanized at 1 and 6 weeks post-surgery.
6
Synthetic and Biological Scaffolds for Muscle Defect Repair
7
Bilateral Ovariectomy in Female Mice
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The IACUC guidelines for survival surgery in rodents and the IACUC Policy on Analgesic Use in Animals Undergoing Surgery were followed for all the procedures. Animal experiments for this work were performed in accordance with the protocol approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Michigan (PRO00007716).
Bilateral ovariectomies were performed on adult female mice (C57BL/6) aged 12–16 weeks. The mice were anesthetized by isoflurane. Carprofen (5 mg/kg. body weight, Rimadyl, Zoetis) was administered subcutaneously for analgesia. The intraperitoneal space was exposed through a midline incision in the abdominal wall secured using an abdominal retractor. The ovaries were removed, and the muscle and skin layer of the abdominal wall were closed with 5/0 absorbable sutures (AD Surgical). The mice recovered in a clean warmed cage and received another dose of Carprofen 12 hours post recovery or as needed.
Bilateral ovariectomies were performed on adult female mice (C57BL/6) aged 12–16 weeks. The mice were anesthetized by isoflurane. Carprofen (5 mg/kg. body weight, Rimadyl, Zoetis) was administered subcutaneously for analgesia. The intraperitoneal space was exposed through a midline incision in the abdominal wall secured using an abdominal retractor. The ovaries were removed, and the muscle and skin layer of the abdominal wall were closed with 5/0 absorbable sutures (AD Surgical). The mice recovered in a clean warmed cage and received another dose of Carprofen 12 hours post recovery or as needed.
8
Mouse Care and Procedure Guidelines
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All mouse protocols were in accordance with European, national and institutional guidelines and approved by the State Office of North Rhine-Westphalia, Department of Nature, Environment and Consumer Protection (LANUV NRW, Germany; animal study protocol AZ 84–02_04_2014_A372). Mice were kept in the specific and opportunistic pathogen free animal facility of the CECAD Research Center, University of Cologne, Germany, in individually ventilated cages (Greenline GM500; Tecniplast) at 22°C (± 2°C) and a humidity of 55% (± 5%) under 12 h light cycle with access to water and food ad libitum. Mice were anesthetized with ketamine (Ketaset, Zoetis) and xylazine (Rompun, Bayer) and euthanized by cervical dislocation. Carprofen (Rimadyl, Zoetis) was used as analgesic after surgery. All efforts were made to minimize suffering.
9
Radiolabeling of Chitosan-Based Nanoparticles
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Low-molecular-weight chitosan (50–190 kDa) and glycol chitosan were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Span® 80 was obtained from Alfa Aesar (Lancashire, UK). All other chemicals were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The instant thin-layer chromatography plate was obtained from Agilent Technologies (Glostrup, Denmark). 111In-InCl3 and 177Lu-LuCl3 were purchased from the Institute of Nuclear Energy (Taoyuan, Taiwan) and ITG Isotope Technologies Garching GmbH (Garching, Germany), respectively. Cell culture medium was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fetal bovine serum was obtained from GE Healthcare—HyClone Laboratories Inc. (Logan, UT, USA). ITLC-SG plate was purchased from Agilent Technologies (Glostrup, Denmark). RIMADYL was purchased from Zoetis (Parsippany, NJ, USA). BAYTRIL was purchased from Bayer HealthCare (Leverkusen, Germany).
10
Wistar Albino Rat Anesthesia Protocol
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In the present study, 28 adult male Wistar Albino rats between 12 and 14 weeks of age were used. Their weight ranged between 250 and 300 g. The animals were provided by the Hellenic Pasteur Institute (Athens, Greece), and were acclimatized in our laboratory for 1 week before the onset of the experiment. The housing conditions were standardized to provide 12-h light and dark cycles, temperature of 22±2°C and relative humidity of 60%. All animals received analgesia with carprofen (0.08 ml/kg; Rimadyl; Zoetis Inc., Florham Park, NJ, USA) and chemoprophylaxis with enrofloxacin (10 mg/kg; Baytril; Bayer AG, Leverkusen, Germany) subcutaneously preoperatively. An intramuscular injection of ketamine (100 mg/kg; Institute of Pharmaceutical Research and Technology, Attica, Greece) and xylazine (5 mg/kg; Rompun; Bayer AG) was used for anesthesia in each animal. All rats breathed spontaneously during the surgical procedures. The skin of their scrotum was shaved and scrubbed with Betadine (Lavipharm SA, Attica, Greece) solution. All procedures were performed under sterile conditions. The experimental protocols conformed to the ethical recommendations of the European Union Directive 2010/63/EU for animal experiments, and were approved by the Veterinary Directorate of the Region of Attica and the Ethics Committee of the Medical School of the University of Athens (Athens, Greece).
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