37 c co2 incubator

Manufactured by Thermo Fisher Scientific

Sourced in United States

The 37°C CO2 incubator is a temperature-controlled laboratory equipment designed to provide a stable and optimized environment for cell culture and other biological applications. It maintains a constant temperature of 37°C and a controlled carbon dioxide (CO2) atmosphere, which are critical parameters for the growth and maintenance of cell lines. The incubator ensures consistent and reliable environmental conditions to support the optimal growth and development of cell cultures.

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Lab products found in correlation

Gaspak ez anaerobe gas generating pouch system, by BD (1 mentions) Penicillin streptomycin, by Sartorius (1 mentions) 2 β mercaptoethanol, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

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CO2 incubator (511 citations) 37°C incubator (35 citations)

3 protocols using 37 c co2 incubator

Culturing Cells in Normoxic and Hypoxic Conditions

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CPC-WT and CPC-KO were cultured in normoxic (O₂ 20%) and hypoxic conditions (O₂ 1%) in a 37°C CO₂ incubator (Thermo Fisher Scientific, Waltham, MA, USA) to perform experiments. The hypoxic condition was generated with BD GasPak™ EZ Anaerobe Gas Generating Pouch System (Becton, Dickinson and Company, Franklin Lakes, NJ, USA).

Seo S.K., Kim N., Lee J.H., Kim S.M., Lee S.Y., Bae J.W., Hwang K.K., Kim D.W., Koch W.J, & Cho M.C. (2018). β-arrestin2 Affects Cardiac Progenitor Cell Survival through Cell Mobility and Tube Formation in Severe Hypoxia. Korean Circulation Journal, 48(4), 296-309.

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Culturing Mouse Pancreatic β-Cells

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The mouse β islet cell line, β-TC-6, was supplied by FuHeng Cell Center (FH0387, Shanghai, China) and tested, shown to be free of mycoplasma and bacterial contamination. All cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) (Hyclone, Life Technologies, USA) with 20% FBS (10099-141, GIBCO, Australia), 0.1% 2-beta-mercaptoethanol (M3148, Sigma-Aldrich Corp, USA), and 1% penicillin/streptomycin (Biological Industries, Israel) and incubated in a 37°C CO 2 incubator (ThermoFisher, USA). Half of the medium was changed every alternate day.

Lan T., Guo J., Bai X., Huang Z., Wei Z., Du G., Yan G., Weng L, & Yi X. (2020). RGD-modified injectable hydrogel maintains islet beta-cell survival and function. Journal of applied biomaterials & functional materials, 18.

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Wound Healing Assay Using Cells

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Confluent monolayers of cells in a 24 well plate were scratched evenly down the middle of each well with a 200 μl pipet tip. Cells were washed with PBS four times to remove any debris. Fresh medium was then added and cells were incubated in a 37°C CO₂ incubator (Thermo) for up to 8 h. Scratch area were imaged at 0, 2, 4, 6, and 8 h following the initial scratch. Bright-field microscopy images were captured using an AM Scope 3.7 digital camera (AmScope). Five random regions per well were used to determine the overall migration ability of cells. ImageJ software version 1.51J (NIH, Bethesda, MD; http://imagej.nih.gov/ij) was used to measure wound closure area at each time point. Image analysis was conducted in triplicate for all cell experiments.

Lam V.K., Sharma P., Nguyen T., Nehmetallah G., Raub C.B, & Chung B.M. (2020). Morphology, Motility, and Cytoskeletal Architecture of Breast Cancer Cells Depend on Keratin 19 and Substrate. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 97(11), 1145-1155.

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