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4 protocols using isorhamnetin 3 glucoside

1

Phytochemical Analysis of Natural Antioxidants

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Sigma-Aldrich (St. Louis, MO, USA) provided 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2′,7′-dichlorofluorescein diacetate (DCF-DA), lipopolysaccharide (LPS), Folin–Ciocalteu (F–C) reagent, gallic acid, AlCl3, Griess reagent, NG-Methyl-L-arginine acetate salt (L-NMMA), WY14643, CaCl2, and HPLC grade standards of quercetin, kaempferol, isorhamnetin, kaempferol 3-glucoside, isorhamnetin 3-glucoside, quercetin 3-glucoside, and quercetin 3,4′-diglucoside. Solvents for extraction and fractionation (ethanol, n-hexane, chloroform, ethyl acetate, and n-butanol) and formic acid were purchased from Daejung Chemicals & Metals (Siheung, Republic of Korea). Solvents for LC/MS (methanol, acetonitrile) were purchased from Honeywell Burdick & Jackson (Muskegon, MI, USA).
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2

Analytical Standards for Diverse Metabolites

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Several standards were used for all individual primary and secondary metabolites. For the sugars sucrose, glucose, and fructose (Sigma–Aldrich Chemie GmbH, Steinheim, Germany); for organic acids oxalic acid, citric acid, malic acid, succinic acid, quinic acid, ascorbic acid, and fumaric acid (Sigma–Aldrich Chemie GmbH, Steinheim, Germany); for individual phenolics caffeic acid, p-coumaric acid, ferulic acid, 5-caffeoxlquinic acid, apigenin-7-glucoside, luteolin-7-glucoside, kaempferol-3-glucoside, quercetin rhamnoside, quercetin rutinoside, and isorhamnetin-3-glucoside (Sigma–Aldrich Chemie GmbH, Steinheim, Germany); and for capsaicnoids capsaicin, dihydrocapsaicin, and nordihydrocapsaicin (Sigma–Aldrich Chemie GmbH, Steinheim, Germany).
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3

HPLC Analysis of Phytochemicals

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Water, acetonitrile, and formic acid were all HPLC grade and purchased
from Fisher Scientific (Fair Lawn, NJ, USA). Trifluoroacetic acid (TFA;
99%) was obtained from Acros Organics (Morris Plains, NJ, USA).
Cyanidin-3-O-glucoside analytical standard (≥
95%), chlorogenic acid (reference standard), rutin (HPLC grade),
neo-chlorogenic acid (analytical standard grade), crypto-chlorogenic acid
(analytical standard grade), quercetin 3-rutinoside (analytical standard grade),
quercetin 3-glucoside (analytical standard grade), kaempferol 3-rutinoside
(analytical standard grade), isorhamnetin 3-rutinoside (analytical standard
grade), and isorhamnetin 3-glucoside (analytical standard grade) were purchased
from Sigma Aldrich (St. Louis, MO, USA).
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4

Analytical Reagents for Polyphenol Analysis

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Methanol (HPLC-grade) was supplied by Lab-Scan (Dublin, Ireland). Folin-Ciocalteu´s phenol reagent, ferulic acid, gallic acid, quercetin, quercetin-3-glucoside, quercetin-3,4′-diglucoside and isorhamnetin-3-glucoside were purchased from Sigma-Aldrich (St Louis, MO, USA).
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