Gd@Cdots were dispersed in Milli-Q H2O (100 μg/mL) and transferred to a quartz cuvette. Absorbance between 200–800 nm was scanned on a Varian Cary 300 bio UV-visible spectrometer. For emission spectrum, Gd@Cdots were dispersed in Milli-Q H2O (100 μg/mL) and placed in a black 96-well plate (Corning Costar, Cat# 3614). The fluorescence spectra were acquired on a microplate reader (Synergy Mx, BioTeK) with excitation at 457, 520, and 570 nm.
Cary 300 bio uv
Manufactured by Agilent Technologies
The Cary 300 bio UV is a UV-Vis spectrophotometer designed for life science applications. It features a wavelength range of 190 to 900 nm and can be used for various analytical tasks such as protein, nucleic acid, and cell density measurements.
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2 protocols using cary 300 bio uv
1
Spectroscopic Analysis of Gd@Cdots
2
Characterization of Nanomaterials
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UV–vis
absorption spectra were acquired
with a Varian Cary 300 BIO UV–vis spectrophotometer equipped
with a temperature controller (±0.1 K). The fluorescence spectra
were recorded by using a Cary Eclipse fluorescence spectrophotometer
(American, Agilent, Co.). The transmission electron microscopy (TEM)
images of the products were taken on a JEOL JEM-200CX transmission
electron microscope, employing an accelerating voltage of 200 kV.
DLS measurements were detected on a BI-200SM laser light scattering
system (Brookhaven Instruments Corporation).
absorption spectra were acquired
with a Varian Cary 300 BIO UV–vis spectrophotometer equipped
with a temperature controller (±0.1 K). The fluorescence spectra
were recorded by using a Cary Eclipse fluorescence spectrophotometer
(American, Agilent, Co.). The transmission electron microscopy (TEM)
images of the products were taken on a JEOL JEM-200CX transmission
electron microscope, employing an accelerating voltage of 200 kV.
DLS measurements were detected on a BI-200SM laser light scattering
system (Brookhaven Instruments Corporation).
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