Accu check

Manufactured by Roche

Sourced in Switzerland, Germany, United States, France, United Kingdom, Australia, Brazil, Spain, Japan, Sweden, Canada, Poland, China

The Accu-Chek is a blood glucose monitoring system designed to measure and display the level of glucose in a person's blood. It provides a convenient and accurate way to monitor blood glucose levels for individuals with diabetes or those who need to regularly track their blood sugar.

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Lab products found in correlation

Streptozotocin (stz), by Merck Group (19 mentions) D glucose, by Merck Group (11 mentions) Actrapid, by Novo Nordisk (8 mentions) Insulin, by Merck Group (6 mentions) Ultra sensitive mouse insulin elisa kit, by Crystal Chem (3 mentions) Streptozotocin (stz), by Roche (3 mentions) Mouse c peptide elisa kit, by ALPCO (2 mentions) Humulin, by Eli Lilly (2 mentions) Ultrasensitive rat insulin elisa system, by Mercodia (2 mentions) Novorapid, by Novo Nordisk (2 mentions) Fuji dri chem 3500, by Fujifilm (2 mentions) Insulatard, by Novo Nordisk (2 mentions) Combur 10 m test stripes, by Roche (2 mentions) Reflotron systems spectrophotometer, by Roche (2 mentions) Insulin, by Mercodia (2 mentions) Vettest 8008, by IDEXX (2 mentions) Free glycerol reagent, by Merck Group (2 mentions) Triglyceride quantification kit, by Abcam (2 mentions) Porcine insulin, by Merck Group (2 mentions) Mouse rat fgf21 elisa kit, by R&D Systems (2 mentions)

Close spelling variants of this product

Accu-Check III Accu-Check Aviva blood glucose monitor Accu-Check glucose meter Accu-Check Go Accu-Check Compact Plus Accu-Check® Avia monitoring system Accu-Check AVIVA test strips Accu-Check Advantage ACCU-CHECK® Performa meter Accu-Check II Glucometer

349 protocols using accu check

Heart Rate, RPE, and Lactate Monitoring

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The heart rate (HR) was monitored (Polar
^®RS800CX, Helsinki, Finland) throughout the entire YoYoIE2. After the YoYoIE2, the participants remained seated wearing the goggles for HR recording (recovery). Just after the YoYoIE2, the player indicated (individually to prevent bias) a score for his rate of perceived exertion (RPE) via the Borg CR10 scale ranging from 0 (“nothing at all”) to 10 (“very very hard”) to determine the internal intensity of the session
3 (link)
. In the 3
^rdminute after the test, a blood sample (25 µL) was collected from the fingertip to measure the lactate concentration using a valid
18 (link)
portable analyzer (ROCHE
^®Accu-Check, Basel, Switzerland).

Londe A.M., Marocolo M., Marocolo I.C., Fisher J., Neto O.B., Souza M.V, & da Mota G.R. (2018). Wearing Colored Glasses can Influence Exercise Performance and Testosterone concentration?. Sports Medicine International Open, 2(2), E46-E51.

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Hyperglycemic Clamp Assay for Insulin Secretion

Cited 1 time

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One-step hyperglycemic clamps were performed on conscious animals as described [41] (link). A 20% dextrose solution was infused through the jugular vein to clamp plasma glucose at 18 mM for 100 min and was adjusted based on glucose measurements (Roche Accu-Check; Roche, Indianapolis, IN). At 90 min, an arginine bolus injection was performed (1 mmol/kg; Sandoz Canada) to assess the maximal insulin response. Plasma samples were collected from the tail at several time points during the clamp for insulin measurements using a mouse insulin enzyme-linked immunosorbent assay (ELISA) kit (Alpco Diagnostics, Salem, NH). Plasma samples for C-peptide measurements were collected at 75 min and analyzed using a mouse C-peptide ELISA kit (Alpco Diagnostics). Two-hour hyperinsulinemic-euglycemic clamps were performed in ASK1 wild-type and knockout mice as previously described [41] (link). Briefly, following a 1-min bolus insulin infusion (85 mU/kg; Humulin R), insulin was infused at 5 mU/kg/min. Twenty percent dextrose was infused starting 5 min after the insulin infusion to clamp glycemia at ∼6.5 mM. The insulin sensitivity index (M/I) was calculated as the glucose infusion rate (M) divided by the average insulinemia during the last 60 min of the clamp (I).

Pepin E., Higa A., Schuster-Klein C., Bernard C., Sulpice T., Guardiola B., Chevet E, & Alquier T. (2014). Deletion of Apoptosis Signal-Regulating Kinase 1 (ASK1) Protects Pancreatic Beta-Cells from Stress-Induced Death but Not from Glucose Homeostasis Alterations under Pro-Inflammatory Conditions. PLoS ONE, 9(11), e112714.

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Glucose and Insulin Tolerance Tests

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Blood glucose was measured in total blood using an Accu-Check glucometer (Roche). Glucose tolerance tests, pyruvate tolerance tests, and insulin tolerance tests were performed in 4-week-old OGT^LKO and control OGT^LWT littermates. For glucose tolerance tests, mice were fasted 4–5 h and received 2 g/kg of glucose; for pyruvate tolerance tests, mice were fasted overnight and received 1 g/kg of pyruvate; and for insulin tolerance tests, mice were fasted 4–5 h and received 0.75 U/kg of insulin. Blood glucose concentrations were measured over a period of 120 min for all tests.

Ortega-Prieto P., Parlati L., Benhamed F., Regnier M., Cavalcante I., Montabord M., Onifarasoaniaina R., Favier M., Pavlovic N., Magusto J., Cauzac M., Pagesy P., Gautheron J., Desdouets C., Guilmeau S., Issad T, & Postic C. (2023). O-GlcNAc transferase acts as a critical nutritional node for the control of liver homeostasis. JHEP Reports, 6(2), 100878.

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Glucose Tolerance Test Protocol

Cited 1 time

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IPGTT was performed at week 16 (data not shown) and week 20 using the Accu-Check glucometer monitor and strips (Roche Diagnostics, West Sussex, UK) [32 (link)]. After an overnight fast, baseline blood glucose (time 0) was determined; then a glucose solution (25%; 2 g/kg, i.p) was injected, followed by the determination of blood glucose levels at 30, 60, and 120 min. The area under the curve of glucose was calculated [33 (link)].

Ibrahim S.M., El- Denshary E.S, & Abdallah D.M. (2015). Geraniol, Alone and in Combination with Pioglitazone, Ameliorates Fructose-Induced Metabolic Syndrome in Rats via the Modulation of Both Inflammatory and Oxidative Stress Status. PLoS ONE, 10(2), e0117516.

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Intraperitoneal Glucose Tolerance Test

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After 8 h fasting, Intraperitoneal Glucose Tolerance Test (IPGTT) was performed through the administration of a glucose solution (2 g/kg) in the rats (n = 8 per group). A hand-held Accu-Check glucometer (Roche Diagnostics, Indianapolis, IN, USA) was used to measure the glucose levels over a 2 h period from the tail veins. The total Area Under Curve (AUC) of the glucose and time was calculated.

Wang Y.Y., Lin S.Y., Chang C.Y., Wu C.C., Chen W.Y., Liao S.L., Chen Y.F., Wang W.Y, & Chen C.J. (2021). Jak2 Inhibitor AG490 Improved Poststroke Central and Peripheral Inflammation and Metabolic Abnormalities in a Rat Model of Ischemic Stroke. Antioxidants, 10(12), 1958.

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Determination of Plasma Biomarkers

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Whole blood was mixed with EDTA-2Na (2 mg/ml) and aprotinin (500 kIU/ml) to determine the plasma levels of FGF15, 5-HT, and insulin. Plasma insulin were measured by enzyme-linked immunosorbent assay (mouse Insulin ELISA Kit [TMB], AKRIN-011T, Shibayagi, Gunma, Japan) as described previously (12 (link)). Plasma levels of FGF15 were measured by enzyme-linked immunosorbent assay (mouse FGF15 ELISA kit, CSB-EL522052MO, WUHAN HUAMEI BIOTECH Co). Plasma 5-HT levels were measured by enzyme-linked immunosorbent assay (mouse 5-HT; BA E-5900, Labor Diagnostika Nord, Nordhorn, Germany). Blood glucose levels were measured using glucose strips (blood glucose monitoring system; Accu-Check, Roche Diagnostics, Tokyo, Japan).

Nonogaki K, & Kaji T. (2023). Ingestion of whey protein and β-conglycinin exerts opposite effects on intestinal FGF15 and serotonin secretion in mice. Frontiers in Endocrinology, 14, 1080790.

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Glucose and Insulin Metabolism in Mice

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The mice were randomly divided into three groups (i.e., control (distilled water), standard noodles, and FLE noodles with fermented lettuce extract). After 30 min of the administration of distilled water or noodles, the mice were orally treated with 2 g/kg of glucose for OGTT or 2 g/kg of sucrose for OSTT (Table 4a,b). Blood samples were collected from the tail vein at 0, 30, 60, 90, and 120 min and blood glucose levels were measured with a blood glucose meter (Accu-check, Roche, Basel, Switzerland).
For FBGT, the mice were given an oral dose of distilled water or noodles (300 mg/kg) and blood samples were collected from the tail vein at 0, 30, 60, 90, and 120 min to measure blood glucose levels (Table 4c).
The fasting blood insulin levels were determined using a mouse insulin enzyme-linked immunosorbent assay kit (Shibayagi Co., Ltd., Gunma, Japan). To evaluate the degree of insulin resistance, HOMA-IR was calculated with the fasting blood glucose and fasting blood insulin values as follows [64 (link)]:
All animal experiments involved groups of five replicates and were repeated three times each with all types of noodles.

Jeong S.Y., Kim E., Zhang M., Lee Y.S., Ji B., Lee S.H., Cheong Y.E., Yun S.I., Kim Y.S., Kim K.H., Kim M.S., Chun H.S, & Kim S. (2021). Antidiabetic Effect of Noodles Containing Fermented Lettuce Extracts. Metabolites, 11(8), 520.

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Glucose and Insulin Tolerance Tests

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For the oral glucose tolerance test (GTT) and insulin tolerance test (ITT), cages were changed, mice were fasted for 6 h, and their weights were measured prior to testing [58] (link). For both tests, the fasting glucose levels were measured at t = 0 via tail vein blood using a glucometer (Accu-Check, Roche). For GTT, 2 g/kg dosage of glucose solution (20% D-glucose in sterile 0.9% NaCl saline solution) was orally administered using flexible plastic feeding tubes with time lapse of 2-3 min between each animal. For ITT, an intraperitoneal injection of insulin (Actrapid, UK) at a dose of 0.5UI/kg, or 0.75UI/kg (for P-HFD and C-HFD) was administered. For both OGTT and ITT, glucose measurements were measured at 15, 30-, 60-, 90-, and 120-mins post administration.

Al-Onaizi M., Braysh K., Alkafeef S.S., Altarrah D., Dannoon S., Alasousi D., Adel H., Al-Ajmi M., Kandari A., Najem R., Nizam R., Williams M.R., John S., Thanaraj T.A., Ahmad R., Al-Hussaini H., Al-Mulla F, & Alzaid F. (2024). Glucose intolerance induces anxiety-like behaviors independent of obesity and insulin resistance in a novel model of nutritional metabolic stress. Nutritional neuroscience.

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Oral Glucose Tolerance Test in Mice

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After 5 h of food deprivation, glucose was administered orally to the mice at a dose of 2.0 g/kg body weight. Blood samples were taken from the tail before and 15, 30, 60, and 120 min after glucose administration, and the blood glucose levels were measured using a blood glucose meter (Accu-Check; Roche Diagnostics, Mannheim, Germany). The blood glucose level before glucose administration represented the fasting glucose level.

Wang J., Tang H., Wang X., Zhang X., Zhang C., Zhang M., Zhao Y., Zhao L, & Shen J. (2016). The structural alteration of gut microbiota in low-birth-weight mice undergoing accelerated postnatal growth. Scientific Reports, 6, 27780.

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Intraperitoneal Glucose Tolerance Test

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Prior to an intraperitoneal glucose tolerance test (IPGTT) (n = 6 per group), rats were deprived of diet for 8 h. The IPGTT was performed through the intraperitoneal administration of glucose solution (2 g/kg body weight). Their blood was then collected from the tail veins over time and glucose levels were measured using a hand-held Accu-Check glucometer (Roche Diagnostics, Indianapolis, IN, USA). The total area under the curve (AUC) for IPGTT was calculated using the trapezoidal (trapezium) rule.

Lin S.Y., Wang Y.Y., Chang C.Y., Wu C.C., Chen W.Y., Kuan Y.H., Liao S.L, & Chen C.J. (2020). Effects of β-Adrenergic Blockade on Metabolic and Inflammatory Responses in a Rat Model of Ischemic Stroke. Cells, 9(6), 1373.

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