SEM observations were performed as described in Sugiura and Matsumoto [14 (link)]. Fifteen minutes after mating, eight females of each species were dehydrated in 100% ethanol for 3 h and then soaked in tertial-butyl alcohol overnight. After being lyophilized with a JFD-320 device (JEOL, Tokyo, Japan), the samples were transferred onto aluminium stubs. The samples were then sputter-coated with gold and observed with a JSM 6510 (JEOL) scanning electron microscope.
Jfd 320 device
Manufactured by JEOL
Sourced in Japan
The JFD-320 is a field-emission scanning electron microscope (FE-SEM) designed for high-resolution imaging and analysis of a wide range of samples. It features a field-emission electron gun that provides a high-intensity, small-diameter electron beam, enabling the JFD-320 to achieve a high resolution of up to 1.0 nm.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using jfd 320 device
1
Scanning Electron Microscopy Protocol
2
Observing Spermatozoa and Oocytes in Multiple Species
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The testicular spermatozoa of at least 10 males were collected from each species and observed. From each species, 15 females that laid eggs were selected for observations of the spermathecal spermatozoa and oocytes. The protocol for preparation and observation with an SEM was that described in Rebecchi and Guidi (1991) .
At least 30 eggs of each species were collected, with at least 15 from eggs 5 min after being laid and at least 15 over 1 day after being laid. Eggs were dehydrated in 100% ethanol for 3 h and then placed in tertiary-butyl alcohol, soaked overnight, and lyophilized using a JFD-320 device (JEOL, Japan).
All samples were transferred onto aluminium stabs, sputter coated with gold, and observed using an SEM, JSM 6510 (JEOL). Person's chi-squared test with default settings (Yates's continuity correction) of 'chisq.test' function of R (R Core Team, 2016) was performed to compare the numbers of eggs containing spermatozoa (row = number of oocytes/laid eggs, column = number of specimens with/without spermatozoa, in each species).
At least 30 eggs of each species were collected, with at least 15 from eggs 5 min after being laid and at least 15 over 1 day after being laid. Eggs were dehydrated in 100% ethanol for 3 h and then placed in tertiary-butyl alcohol, soaked overnight, and lyophilized using a JFD-320 device (JEOL, Japan).
All samples were transferred onto aluminium stabs, sputter coated with gold, and observed using an SEM, JSM 6510 (JEOL). Person's chi-squared test with default settings (Yates's continuity correction) of 'chisq.test' function of R (R Core Team, 2016) was performed to compare the numbers of eggs containing spermatozoa (row = number of oocytes/laid eggs, column = number of specimens with/without spermatozoa, in each species).
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