Leica mz8 stereo microscope

For motility assays, cells were grown in CTT medium, harvested, and resuspended in 1% CTT to a calculated density of 7 × 10⁹ cells ml⁻¹. Five-microliter aliquots of cell suspensions were placed on 0.5% and 1.5% agar supplemented with 0.5% CTT and incubated at 32 °C. After 24 h, colony edges were observed using a Leica MZ8 stereomicroscope or a Leica IMB/E inverted microscope and visualized using Leica DFC280 and DFC350FX charge-coupled-device cameras, respectively. For development assay, cells were harvested and resuspended in MC7 buffer (10 mM MOPS pH 7.0, 1 mM CaCl₂) to a calculated density of 7 × 10⁹ cells ml⁻¹. Twenty microliter aliquots of cells were placed on TPM agar (10 mM Tris-HCl pH 7.6, 1 mM K₂HPO₄/KH₂PO₄ pH 7.6, 8 mM MgSO₄); for development in submerged culture, 50 μl of the cell suspension were mixed with 350 μl MC7 buffer and placed in an 18 mm diameter microtiter dish. Cells were visualized at the indicated time points using a Leica MZ8 stereomicroscope or a Leica IMB/E inverted microscope and imaged using Leica DFC280 and DFC350FX CCD cameras, respectively. Sporulation levels were determined after development for 120 h in submerged culture as the number of sonication- and heat-resistant spores relative to WT.

Two-month-old mice of each genotype were administered a lethal dose of pentobarbital (Dolethal, Vétoquinol, Madrid, Spain). The inner ear and the ossicles — the malleus, incus, and stapes — of the middle ear were dissected as reported (Sakamoto et al., 2017 (link)). Microphotographs of these structures were taken using a digital camera connected to a Leica MZ8 stereo microscope (Leica, Wetzlar, Germany).

Three P20 mice of each genotype were administered a lethal dose of pentobarbital (Dolethal, Vétoquinol). The ossicles—the malleus, incus, and stapes—of the middle ear were dissected. Microphotographs of these structures were taken using a digital camera connected to a Leica MZ8 stereo microscope (Leitz).