Sola se 2 365

Manufactured by Lumencor

Sourced in Japan

The Sola SE II 365 is a light engine that provides broad-spectrum illumination. It produces light in the wavelength range of 365 nanometers.

Automatically generated - may contain errors

Lab products found in correlation

Ff444 521 608 di01 dichroic beamsplitter, by IDEX Corporation (3 mentions) Fura red, by Thermo Fisher Scientific (3 mentions) Orca flash4.0 v2 digital cmos camera, by Hamamatsu Photonics (3 mentions) Solution and chamber heaters, by Warner Instruments (2 mentions) Eclipse ti inverted microscope, by Nikon (2 mentions) Glass bottomed imaging chamber, by Warner Instruments (2 mentions) Na superfluor objective, by Nikon (2 mentions) Furared, by IDEX Corporation (2 mentions) Nis element, by Nikon (2 mentions) Alveole primo, by Nikon (2 mentions) Orca flash 4.0 lt b w camera, by Hamamatsu Photonics (2 mentions) Evos fl microscope, by Thermo Fisher Scientific (1 mentions) Ax10 observer d1, by Zeiss (1 mentions) Apochromat 20, by Zeiss (1 mentions) Matlab software, by MathWorks (1 mentions) Custom matlab software, by MathWorks (1 mentions) Facsmelody cell sorter, by BD (1 mentions) Inline solution and chamber heaters, by Warner Instruments (1 mentions) Eclipse ti e inverted microscope, by Nikon (1 mentions)

7 protocols using sola se 2 365

Automated Epifluorescent Microscopy Imaging

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Images described on Fig EV1B are acquired on an EVOS FL Microscope (Thermo Fisher) equipped with a GFP LED light cube. Images described on Fig EV1C are acquired on a Zeiss Ax10 Observer D1 fluorescence microscope system equipped with AxioCam and an HXP 120V lamp, with an Objective Plan‐Apochromat 20× and ZEN acquisition software.
All other fluorescent images are acquired on a Nikon Ti‐E automated epifluorescent microscope. The microscope was equipped with a DS‐Qi2 camera and a Lumencor Sola SE II 365 LED lamp. The filters sets were provided by Nikon for DAPI (DAPI‐5060C), Alexa Fluor 488 (FITC‐3540C), and Alexa Fluor 568 (mCherry C M343564, Sembrock)). The objectives used were Apo 20× Lambda, numerical aperture 0.75 (Nikon). We used a hardware‐based autofocusing system called the perfect focus system (PFS) from Nikon to automatically focus the cells in the field of view.

Serçin Ö., Reither S., Roidos P., Ballin N., Palikyras S., Baginska A., Rein K., Llamazares M., Halavatyi A., Winter H., Muley T., Jurkowska R.Z., Abdollahi A., Zenke F.T., Neumann B, & Mardin B.R. (2019). A solid‐phase transfection platform for arrayed CRISPR screens. Molecular Systems Biology, 15(12), e8983.

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Cytosolic Calcium Imaging of Pancreatic Islets

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For measurements of cytosolic Ca²⁺, islets were pre-incubated in 2.5 μM FuraRed (Molecular Probes F3020) in islet media for 45 min at 37°C before they were placed in a glass-bottomed imaging chamber (Warner Instruments) mounted on a Nikon Ti-Eclipse inverted microscope equipped with a 20X/0.75NA SuperFluor objective (Nikon Instruments). The chamber was perfused with a standard external solution containing 135 mM NaCl, 4.8 mM KCl, 2.5 mM CaCl₂, 1.2 mM MgCl₂, 20 mM HEPES (pH 7.35). The flow rate was 0.4 mL/min and temperature was maintained at 33°C using solution and chamber heaters (Warner Instruments). Excitation was provided by a SOLA SEII 365 (Lumencor) set to 10% output. Single DiR images utilized a Chroma Cy7 cube (710/75x, T760lpxr, 810/90 m). Excitation (430/20x and 500/20x) and emission (630/70 m) filters (ET type; Chroma Technology) were used in combination with an FF444/521/608-Di01 dichroic beam splitter (Semrock) and reported as the excitation ratio (R430/500). Fluorescence emission was collected with a Hamamatsu ORCA-Flash4.0 V2 Digital CMOS camera every 6 s. A single region of interest was used to quantify the average response of each islet using Nikon Elements and custom MATLAB software (MathWorks).

Abulizi A., Cardone R.L., Stark R., Lewandowski S.L., Zhao X., Hillion J., Ma L., Sehgal R., Alves T.C., Thomas C., Kung C., Wang B., Siebel S., Andrews Z.B., Mason G.F., Rinehart J., Merrins M.J, & Kibbey R.G. (2020). Multi-Tissue Acceleration of the Mitochondrial Phosphoenolpyruvate Cycle Improves Whole-Body Metabolic Health. Cell metabolism, 32(5), 751-766.e11.

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Fluorescence Imaging of Islet Calcium Dynamics

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For measurements of cytosolic Ca²⁺, islets were pre-incubated in 2.5 μM FuraRed (Molecular Probes F3020) in islet media for 45 min at 37°C before they were placed in a glass-bottomed imaging chamber (Warner Instruments) mounted on a Nikon Ti-Eclipse inverted microscope equipped with a 20X/0.75NA SuperFluor objective (Nikon Instruments). The chamber was perfused with a standard external solution containing 135 mM NaCl, 4.8 mM KCl, 2.5 mM CaCl₂, 1.2 mM MgCl₂, 20 mM HEPES (pH 7.35). The flow rate was 0.4 mL/min and temperature was maintained at 33°C using solution and chamber heaters (Warner Instruments). Excitation was provided by a SOLA SEII 365 (Lumencor) set to 10% output. Single DiR images utilized a Chroma Cy7 cube (710/75x, T760lpxr, 810/90m). Excitation (x) or emission (m) filters (ET type; Chroma Technology) were used in combination with an FF444/521/608-Di01 dichroic beamsplitter (Semrock) as follows: FuraRed, 430/20x and 500/20x, 630/70m (R430/500); NAD(P)H, 365/20x, 470/24m; Rhodamine-123 and Glutamate, 500/20x, 535/35m; and Perceval-HR and SypHer mt, 430/20x and 500/20x, 535/35m (R500/430). Fluorescence emission was collected with a Hamamatsu ORCA-Flash4.0 V2 Digital CMOS camera every 6 s. A single region of interest was used to quantify the average response of each islet using NIS-Elements (Nikon Instruments) and custom MATLAB software (MathWorks).

Lewandowski S.L., Cardone R.L., Foster H.R., Ho T., Potapenko E., Poudel C., VanDeusen H.R., Sdao S.M., Alves T.C., Zhao X., Capozzi M.E., de Souza A.H., Jahan I., Thomas C.J., Nunemaker C.S., Davis D.B., Campbell J.E., Kibbey R.G, & Merrins M.J. (2020). Pyruvate kinase controls signal strength in the insulin secretory pathway. Cell metabolism, 32(5), 736-750.e5.

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Imaging Microfluidic Chip Fluorescence

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Chips were imaged under a fluorescence microscope Nikon Ti-E with Alveole Primo (Nikon, Tokyo, Japan) connected to Hamamatsu Orca Flash 4.0 LT B&W camera (Hamamatsu Photonics, Hamamatsu, Japan) and Lumencor Sola SE II 365 (Lumencor, Beaverton, Oregon, USA). Images for the following transmitted light and fluorescent filters were acquired: DAPI (Semrock 5060C, excitation 377/50, emission 447/60), GFP (Semrock 3035D-NTE, excitation 472/30, emission 520/35), TxRed (Semrock 4040C, excitation 531/40, emission 593/40), and Cy5 (excitation 640/20, emission 700/75). Multiple chips were placed on a microscope slide adapter. The NIS-Elements Advanced Research program was automated to image at 20x magnification and to scan 10 images horizontally to form a complete representation of the channel producing a multichannel composite image. Images of the chips were acquired daily to confirm opening of the microchannels after 24 hours of incubation (Figure 1D).

Sieviläinen M., Saavalainen J., Adnan-Awad S., Salo T, & Al-Samadi A. (2022). IDO1 Inhibition Reduces Immune Cell Exclusion Through Inducing Cell Migration While PD-1 Blockage Increases IL-6 and -8 Secretion From T Cells in Head and Neck Cancer. Frontiers in Immunology, 13, 812822.

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Multiparametric Cell Analysis by Flow Cytometry

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Sorting was performed on a Becton-Dickinson FACSMelody cell sorter equipped with blue, red, and violet lasers (9 color configuration). Cell imaging was done on a Nikon Eclipse Ti2 fluorescent microscope with a Chroma-49004 or Chroma-49006 filter cubes, Lumencor Sola SE II 365 illumination, and an integral CO₂ incubator. Scans were analyzed using NIS Elements AR_software. Cells were identified by Hoechst staining and apoptosis was determined upon co-location with CellEvent™ Caspase-3/7 Green Detection Reagent labeling (see Supplementary Note 10 for additional markers evaluated).

Mamet N., Amir Y., Lavi E., Bassali L., Harari G., Rusinek I., Skalka N., Debby E., Greenberg M., Zamir A., Paz A., Reiss N., Loewenthal G., Avivi I., Shimoni A., Neev G., Abu-Horowitz A, & Bachelet I. (2020). Discovery of tumoricidal DNA oligonucleotides by response-directed in vitro evolution. Communications Biology, 3, 29.

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Automated Imaging of Microfluidic Chips

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Chips were imaged daily under a fluorescent microscope using Nikon Ti-E with Alveole Primo (Nikon, Tokyo, Japan) connected to a Hamamatsu Orca Flash 4.0 LT B&W camera (Hamamatsu Photonics, Hamamatsu, Japan) and Lumencor Sola SE II 365 (Lumencor, Beaverton, USA). Images for the following transmitted light and fluorescent filters were acquired: DAPI (Semrock 5060C, excitation 377/50, emission 447/60), GFP (Semrock 3035D-NTE, excitation 472/30, emission 520/35), and Cy5 (excitation 640/20, emission 700/75). Chips were placed on a microscope slide adaptor. The NIS-Elements Advanced Research program was automated to image at 20x magnification and to scan 10 images horizontally to form a complete representation of the channel producing a multichannel composite image.

Wahbi W., Korelin K., Sieviläinen M., Karihtala P., Wilkman T., Tarkkanen J., Salo T, & Al-Samadi A. (2023). Evaluation of in vitro and in vivo personalized cancer treatment assays for oral squamous cell carcinoma. Translational Oncology, 33, 101677.

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Cytosolic Ca2+ Imaging in Islets

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For measurements of cytosolic Ca²⁺, islets were preincubated in 2.5 μmol/L Fura Red (Molecular Probes, Eugene, OR) for 45 min at 37°C. Islets were then placed in an RC-24N glass-bottom chamber (54 μL volume) (Warner Instruments) on a Nikon Eclipse Ti-E inverted microscope equipped with Super Fluor 10×/0.5 NA and 20×/0.75 NA objectives (Nikon Instruments). The chamber was perfused with standard external solution (as previously described). The flow rate was 0.3 mL/min, and temperature was maintained at 33°C using inline solution and chamber heaters (Warner Instruments). Excitation was provided by a SOLA SE II 365 (lumencor) set to 10% output. Excitation (x) or emission (m) filters (ET type; Chroma Technology) were used in combination with an FF444/521/608-Di01 dichroic beamsplitter (Semrock) as follows: Fura Red 430/20x and 500/20x, 630/70m (R430/500); NAD(P)H 365/20x, 470/24m; and PercevalHR 430/20x and 500/20x, 535/35m (R500/430). Fluorescence emission was collected with a Hamamatsu ORCA-Flash4.0 V2 Digital CMOS camera at 0.125–0.2 Hz. A single region of interest was used to quantify the average response of each islet using Nikon NIS-Elements and MathWorks MATLAB software.

Gregg T., Poudel C., Schmidt B.A., Dhillon R.S., Sdao S.M., Truchan N.A., Baar E.L., Fernandez L.A., Denu J.M., Eliceiri K.W., Rogers J.D., Kimple M.E., Lamming D.W, & Merrins M.J. (2016). Pancreatic β-Cells From Mice Offset Age-Associated Mitochondrial Deficiency With Reduced KATP Channel Activity. Diabetes, 65(9), 2700-2710.

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