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Triple quad ms ms system

Manufactured by AB Sciex
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The Triple Quad MS/MS system is a highly sensitive and selective mass spectrometry instrument designed for quantitative and qualitative analysis. It utilizes tandem mass spectrometry (MS/MS) to provide precise measurements of target analytes in complex samples.

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Lab products found in correlation

Fastap thermosensitive alkaline phosphatase, by Thermo Fisher Scientific (2 mentions) Nuclease p1, by Merck Group (2 mentions) High performance liquid chromatography (hplc), by Agilent Technologies (2 mentions)

Spelling variants of this product

Triple Quad 5500 LC-MS/MS system (11 citations) Triple Quad 5500+ MS/MS system (6 citations) SCIEX Triple Quad 5500 LC-MS/MS System (3 citations) SCIEX Triple Quad 5500+ LC-MS/MS System- QTRAP (2 citations) 6500+ Triple Quad LC-MS/MS System (2 citations)

2 protocols using triple quad ms ms system

1

mRNA Purification and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
We first purified mRNA by two rounds of polyA selection. Fifty ng of purified mRNA were subject to digestion by nuclease P1 (Sigma #N8630–1VL) at 42 degrees for 2 hours followed by phosphatase treatment using FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher #EF0651) at 37 degrees for 1 hour. The digested nucleotides were then analyzed by ruling through a C18 reverse phase column on HPLC (Agilent) and quantified by triple quad MS/MS system (Sciex).
De Jesus D.F., Zhang Z., Kahraman S., Brown N.K., Chen M., Hu J., Gupta M.K., He C, & Kulkarni R.N. (2019). m6A mRNA Methylation Regulates Human β-Cell Biology in Physiological States and in Type 2 Diabetes. Nature metabolism, 1(8), 765-774.
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2

mRNA Purification and Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
We first purified mRNA by two rounds of polyA selection. Fifty ng of purified mRNA were subject to digestion by nuclease P1 (Sigma #N8630–1VL) at 42 degrees for 2 hours followed by phosphatase treatment using FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher #EF0651) at 37 degrees for 1 hour. The digested nucleotides were then analyzed by ruling through a C18 reverse phase column on HPLC (Agilent) and quantified by triple quad MS/MS system (Sciex).
De Jesus D.F., Zhang Z., Kahraman S., Brown N.K., Chen M., Hu J., Gupta M.K., He C, & Kulkarni R.N. (2019). m6A mRNA Methylation Regulates Human β-Cell Biology in Physiological States and in Type 2 Diabetes. Nature metabolism, 1(8), 765-774.
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