Human normal thyroid cell line Nthy-ori3-1 and human thyroid carcinoma cell lines TPC-1, TT, and ARO were obtained from CoBioer Biosciences Co. Ltd. (Nanjing, Jiangsu, China). Cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin, and 100 μg/ml streptomycin. Cells were grown at 37°C in a humidified atmosphere of 95% air and 5% CO2 [29 (link)]. Confluent cells were starved overnight in a serum-free RPMI 1640 medium. The cells were then treated with 10, 25, 50, 100, and 200 μM NaHS (an H2S donor, Sigma-Aldrich, St. Louis, MO, USA) for 24 h. The phosphate-buffered saline (PBS) group was served as a control.
Nthy ori3 1
Manufactured by Cobioer Biosciences
Sourced in China
Nthy-ori3-1 is a cell line derived from normal human thyroid cells. It is commonly used as a model system for studying thyroid biology and diseases. The cell line maintains key characteristics of normal thyroid cells and can be utilized for various research applications.
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Lab products found in correlation
4 protocols using nthy ori3 1
1
Thyroid Cell Lines and Hydrogen Sulfide Treatment
2
Culturing Thyroid Cell Lines
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Human thyroid carcinoma cell lines (TPC-1 and FTC-133) and thyroid epithelial cell line Nthy-ori3-1 were obtained from Cobioer Biosciences (Nanjing, Jiangsu, China). All cells were cultured in Roswell Park Memorial Institute-1640 medium (Gibco, Hangzhou, China) containing 10% fetal bovine serum (Gibco Invitrogen, Grand Island, NY, United States), 100 µg/mL streptomycin, and 100 U/mL penicillin.
3
Culturing Human Thyroid Cell Lines
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The human thyroid carcinoma cell lines, CAL-62 and SW579, were obtained from The Cell Bank of Type Culture Collection of The Chinese Academy of Sciences. The immortalized normal human thyroid follicular epithelial cell line, Nthy-ori3-1, was purchased from CoBioer Biosciences Co., Ltd. (cat. no. CBP61205). HUVECs were purchased from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. HUVECs, CAL-62 and SW579 cells were cultured in DMEM supplemented with 1% penicillin/streptomycin and 10% FBS, at 37°C (5% CO2) in a humidified atmosphere. Nthy-ori3-1 cells were maintained in RPMI-1640 with 10% FBS and 1% penicillin/streptomycin.
4
Cell Line Maintenance for Thyroid Cancer Research
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Human thyroid carcinoma cell lines (TC, ARO, TPC-1, FTC-133) and thyroid epithelial cell line Nthy-ori3-1 were obtained from Cobioer Biosciences (Nanjing, Jiangsu, China). Cells were maintained in RPMI-1640 medium (Gibco, Hangzhou, China) supplemented with 10% fetal bovine serum (Gibco Invitrogen, Grand Island, NY, United States), 100 μg/ml streptomycin, and 100 U/ml penicillin. All cell lines were authenticated by Short Tandem Repeat (STR) assay and cultured at 37 °C in a humidified atmosphere of 5% CO2.
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