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Anti p18ink4c

Manufactured by Cell Signaling Technology
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Sourced in China, United Kingdom, United States

Anti-p18INK4C is a primary antibody that recognizes the p18INK4C protein. It is used to detect and analyze the expression of p18INK4C in various cellular and experimental contexts.

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Lab products found in correlation

Anti cdk4, by Cell Signaling Technology (2 mentions) Anti cdk6, by Cell Signaling Technology (2 mentions) P ask1, by Cell Signaling Technology (1 mentions) Supersignal west dura extended duration substrate, by Thermo Fisher Scientific (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti dnmt3a, by Abcam (1 mentions) Mouse anti cyclin d1, by Cell Signaling Technology (1 mentions) Cell cycle regulation sampler kit, by Cell Signaling Technology (1 mentions) Anti cdkn1b, by Cell Signaling Technology (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Anti cdk2, by Cell Signaling Technology (1 mentions) Anti n cadherin, by Cell Signaling Technology (1 mentions) Anti β catenin, by Cell Signaling Technology (1 mentions) Anti cyclin d1, by Cell Signaling Technology (1 mentions) Anti p27kip1, by Cell Signaling Technology (1 mentions) Anti zo 1, by Merck Group (1 mentions) Anti e cadherin, by Merck Group (1 mentions) Anti hbp1, by Merck Group (1 mentions) Anti vimentin, by Cell Signaling Technology (1 mentions) Anti cyclin d3, by Cell Signaling Technology (1 mentions)

3 protocols using anti p18ink4c

1

Western Blot Analysis of Signaling Proteins

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Cell lysates were harvested by RIPA lysis buffer on ice. An equal amount of 20 μg total cell lysates were separated on 8%‐12% SDS‐PAGE gel and electrophoretically transferred to polyvinylidene fluoride (PVDF) membranes. Following blocking with 5% BSA at room temperature for 2 hours, membranes were then incubated with primary antibodies overnight at 4°C. Finally, membranes were detected with the SuperSignal West Dura Extended Duration Substrate (Thermo Scientific). The following antibodies were used to explore protein expression: anti‐SLC35F2 antibodies (AV43971, Sigma‐Aldrich), anti‐β‐actin (#4970), anti‐CDK2 (#2546), anti‐CDK4 (#12790), anti‐CDK6 (#13331), anti‐Cyclin D1 (#2978), anti‐Cyclin D3 (#2936), anti‐p27 Kip1 (#3686), anti‐p21 Waf1/Cip1 (#2947), anti‐p18 INK4C (#2896), anti‐ERK1/2 (#4695), anti‐p‐ERK1/2 (Thr202/Tyr204, #4370), anti‐JNK (#9252), anti‐p‐JNK (Thr183/Tyr185, #4668), anti‐p38 MAPK (#8690), anti‐p‐p38 MAPK (Thr180/Tyr182, #4511), anti‐ASK‐1 (#8662), anti‐phosphorylation of apoptosis signal‐regulating kinase 1 (p‐ASK‐1) (Thr845, #3765) (Cell Signaling Technology) and anti‐TGFBR1 (CY2905, Abways, China).
He J., Jin Y., Zhou M., Li X., Chen W., Wang Y., Gu S., Cao Y., Chu C., Liu X, & Zou Q. (2018). Solute carrier family 35 member F2 is indispensable for papillary thyroid carcinoma progression through activation of transforming growth factor‐β type I receptor/apoptosis signal‐regulating kinase 1/mitogen‐activated protein kinase signaling axis. Cancer Science, 109(3), 642-655.
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2

Western Blot Analysis of Cell Cycle Regulators

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Western blots were performed using anti-DNMT3A (Abcam, Cambridge, UK) and mouse anti-cyclinD1, anti-CDK4, anti-CDK6, anti-p18INK4C, and anti-CDKN1B which were purchased as part of a Cell Cycle Regulation Sampler Kit (Cell Signaling Technology). Mouse anti-β-actin was obtained from Sigma-Aldrich. Protein detection was performed with Super Signal Chemiluminescence Substrate (Pierce, USA).
Cui H., Zhao C., Gong P., Wang L., Wu H., Zhang K., Zhou R., Wang L., Zhang T., Zhong S, & Fan H. (2015). DNA methyltransferase 3A promotes cell proliferation by silencing CDK inhibitor p18INK4C in gastric carcinogenesis. Scientific Reports, 5, 13781.
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3

Western Blot Analysis of Protein Expression

Cited 1 time
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HK1, HNE1, and CNE2 cell lines were transfected as described above. After 48 h transfection, proteins were extracted in the defined volume of RIPA lysis buffer containing 1.0 mM PMSF, protease inhibitor cocktail and DTT, and 50 μg proteins was loaded in SDS-PAGE gel electrophoresis10 (link). The proteins were transferred to PVDF Membrane (Milipore, Darmstadt, Germany) which were incubated with following primary antibodies: anti-HBP1 (Milipore, Darmstadt, Germany), anti-ZO-1, anti-E-cadherin, anti-N-cadherin, anti-Vimentin, anti-β-catenin, anit-MMP9, anti-NF-κB, anti-caspase 3 and cleaved caspase 3, anti-caspase 7, anti-PARP and cleaved PARP, anti-caspase 9 and cleaved caspase 9, anti-p18INK4C (p18), anti-p21Waft/Cip1 (p21), anti-p27Kip1 (p27), anti-Cyclin D1, anti-Cyclin D3, anti-CDK2, anti-CDK4, anti-CDK6 (Cell Signaling Technology, Danvers MA, USA), and β-actin (ABclonal, Cambridge, MA, USA) as an internal reference. Then the PVDF Membranes were incubated with HRP-linked anti-Rabbit or anti-Mouse IgG antibody according to the isotypes of the primary antibody. The membrane was imaged using ChemiDoc MP System (Bio-Rad, Hercules, CA, USA) and the images were analyzed using Image Lab Software (Bio-Rad, CA, USA).
He S., Yang S., Niu M., Zhong Y., Dan G.a.o., Zhang Y., Ma H., Xiong W., Zhou M., Zhou Y., Xiang B., Li G., Shuai C, & Peng S. (2018). HMG-box transcription factor 1: a positive regulator of the G1/S transition through the Cyclin-CDK-CDKI molecular network in nasopharyngeal carcinoma. Cell Death & Disease, 9(2), 100.
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