After mutant lines and RNAi knockdown progeny were scored for pigmentation, they were preserved in a 3:1 ethanol/glycerol solution and stored at 4°C until dissection for imaging. The fly cuticles were dissected from the abdomen and mounted to a glass slide using Permount and a glass cover slip. All photographs were taken with an Olympus DP25 microscope camera on an Olympus SZ61 stereo microscope.
Dp25 microscope camera
Manufactured by Olympus
Sourced in Japan
The DP25 is a microscope camera designed by Olympus. It features a high-resolution CMOS sensor for capturing detailed images of specimens under a microscope. The camera connects to a computer or other display device to allow for live viewing and image capture.
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Lab products found in correlation
4 protocols using dp25 microscope camera
1
Fly Cuticle Preservation and Imaging
2
Prussian Blue Staining for Intracellular Iron
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The Prussian blue histological staining method was utilized to detect intracellular iron concentrations [90 (link),91 (link)]. RAW 264.7 cells in RPMI (1.5 mL) were plated into a cover glass at 3 × 105 cells/well in 6-well plates. Cells were exposed to MBG and MBG-naproxen at all concentrations (25, 50, and 100 µg.mL−1) for 24 and 48 h. The cells were fixed in 4% paraformaldehyde for 30 min and then washed twice with 1× PBS. Prussian blue solution (equal volumes of 13% HCl and 10% aqueous solution of potassium ferrocyanide) was added to each well for 30 min. After staining, any ferric iron (Fe3+) present in the cells was revealed as a blue pigment. Cells were counterstained with neutral red dye for 5 min to enhance the visualization and distinction between cellular structures and iron deposits. The results were visualized with a compound light microscope (Olympus BX40 microscope) and registered with an Olympus DP25 microscope camera in the magnification of 1000×.
3
Preparation and Analysis of Fragmented Erythrocytes
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The whole-blood samples from five volunteers were drawn into blood collection tubes with K2-EDTA (Terumo, Tokyo, Japan). Pure platelet-rich plasma (PRP) was prepared by centrifugation as described previously [21 (link)]. After collecting PRP, erythrocytes were collected from the rest of the blood sample using the Lymphocyte Separation Solution d = 1.119 (Nacalai Tesque, Kyoto, Japan). Then, the cells were washed with phosphate-buffered saline (PBS). Washed erythrocytes were then heated at 50°C for 15 min to prepare fragmented erythrocytes. These samples were examined with the automated hematology analyzer XN-2000 (Sysmex) and XE-2100 (Sysmex). These samples were stained with May-Grünwald-Giemsa staining solution (Muto Pure Chemicals, Tokyo, Japan) and photographed using a BX-50 microscope with a DP25 microscope camera (Olympus, Tokyo, Japan).
4
Isolation and Characterization of Entomophthoroid Fungi
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Plant debris was collected from Tiantangzhai National Forest Parks (31°17'48" N, 115°78'18" 30°30'57" N, 118°42'17" E PDA ; potato 200 g, dextrose 20 g, agar 20 g, H2O 1000 ml) was inverted over the plant debris and incubated at 21 °C. We surveyed the PDA canopy daily for entomophthoroid fungi, which were transferred to new PDA for purification when detected. Morphological characters of mycelia, primary conidiophores, primary and secondary conidia, and resting spores were described with the method of King (1976a) (link). The length and width of 35 primary conidia, 35 conidiophores and 50 azygospores were measured using an Olympus BX50 research microscope, and then photographed by an Olympus DP25 microscope-camera. Meanwhile, we observed the morphology of secondary conidia grown on 2% agar plates (agar 20 g, H2O 1000 ml) under a light microscope (Olympus BX50, Japan). The living culture was deposited in the Research Center for Entomogenous Fungi of Anhui Agricultural University , Anhui Province, China (RCEF ), and duplicated in the China General Microbiological Culture Collection Center , Beijing, China (CGMCC ). The dried cultures were deposited in the Herbarium Mycologicum Academiae Sinicae, Beijing, China (HMAS ).
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