The Metabolic Syndrome was defined by the Joint Interim Statement criteria as the co-occurrence of at least three out of the following five cardio-metabolic abnormalities: abdominal obesity, hypertension, hyperglycemia, hypertriglyceridemia, and low HDL-cholesterol. The detailed criteria and ethnic-specific cut-offs were described in
In both populations, waist circumference was measured halfway between the iliac crest and the lowest rib using a flexible steel tape measure to the nearest 0.1 cm (SECA Model 201, Seca Gmbh Co, Hamburg, Germany). Blood pressure was obtained by a digital sphygmomanometer at the left arm, at the upright sitting position, after 5 min rest (HEM-7200, Omron Healthcare Co, Ltd, Kyoto, Japan). The average of three measurements was used for analysis.
In the NEO Study, fasting plasma glucose was determined using a standard clinical chemistry method (Roche Modular P800 Analyzer, Roche Diagnostics, Mannheim, Germany). In the SUGARSPIN Study, glucose concentrations were measured with fingertip capillary blood test (BreezeÒ2 glucose meter, Bayer Health Care LLC, Basel, Switzerland). In both the NEO and SUGARSPIN studies, analyses of cholesterol and triglyceride concentrations were performed at the LUMC and determined based on enzymatic colorimetric methods (Roche Modular P800 Analyzer, Roche Diagnostics, Mannheim, Germany).
In both populations, waist circumference was measured halfway between the iliac crest and the lowest rib using a flexible steel tape measure to the nearest 0.1 cm (SECA Model 201, Seca Gmbh Co, Hamburg, Germany). Blood pressure was obtained by a digital sphygmomanometer at the left arm, at the upright sitting position, after 5 min rest (HEM-7200, Omron Healthcare Co, Ltd, Kyoto, Japan). The average of three measurements was used for analysis.
In the NEO Study, fasting plasma glucose was determined using a standard clinical chemistry method (Roche Modular P800 Analyzer, Roche Diagnostics, Mannheim, Germany). In the SUGARSPIN Study, glucose concentrations were measured with fingertip capillary blood test (BreezeÒ2 glucose meter, Bayer Health Care LLC, Basel, Switzerland). In both the NEO and SUGARSPIN studies, analyses of cholesterol and triglyceride concentrations were performed at the LUMC and determined based on enzymatic colorimetric methods (Roche Modular P800 Analyzer, Roche Diagnostics, Mannheim, Germany).