Rabbit anti mycn

For hematoxylin and eosin (H&E) and immunohistochemistry (IHC) stains, brain tissue was fixed in 4% paraformaldehyde/PBS for at least 12 h. The tissue was dehydrated, embedded in paraffin, and sectioned at 4 μm according to standard protocols. All IHC stains were performed on a Ventana System (Roche) using standard protocols. The following antibodies were used: mouse anti-BrdU (Invitrogen, clone MoBU-1, #B35128, 1:100), rabbit anti-Brg1 (Abcam, ab110641, 1:200), rabbit anti-cleaved Caspase 3 (cl. Casp3; Asp175; Cell Signaling Technology, #9664, 1:100), rabbit anti-MycN (Cell Signaling, #51705, 1:100), mouse anti-Pax6 (DSHB, 1:50), mouse anti-phospho-Histone H3 (pHH3, Cell Signaling Technology, #9706, 1:200), rabbit anti-S100 (DAKO, Z0311, 1:100).

miRNA mimics and negative control oligo were purchased from Dharmacon. Anti-miR-506-3p and anti-miR-449a were from Ambion, Inc. siRNAs against MYCN were purchased from Sigma. Rabbit anti-MYCN was purchased from Cell Signaling. Rabbit anti-GAP43, anti-NSE and anti-βIII-tubulin antibodies were obtained from Abcam. Rabbit anti-calnexin antibody, and goat anti-rabbit secondary antibody conjugated with horseradish peroxidase (HRP) were from Santa Cruz (Dallas, TX, USA).