HMWC with approximately 2,000 kDa, glucosamine hydrochloride (GlcN) and N-acetyl glucosamine (GlcNAc) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Trypan blue and chitosanase from Streptomyces griseus were purchased from Sigma Chemical Co. Potato dextrose broth (PD) was purchased from Difco™ (Becton Dickinson, Cockeysville, MD, USA). MTT, WST, thiabendazole and imazalil were also purchased from Sigma Chemical Co. All other reagents were used without further purification and were of the highest grade available.
Chitosanase from streptomyces griseus
Manufactured by Merck Group
Sourced in United States, China
Chitosanase from Streptomyces griseus is an enzyme that hydrolyzes the glycosidic bonds in chitosan, a linear polysaccharide composed of randomly distributed β-(1-4)-linked D-glucosamine and N-acetyl-D-glucosamine units. The enzyme is derived from the bacterium Streptomyces griseus.
Automatically generated - may contain errors
Lab products found in correlation
Imazalil, by Merck Group (1 mentions)
Thiabendazole, by Merck Group (1 mentions)
Trypan blue, by Merck Group (1 mentions)
Glucosamine hydrochloride glcn, by Merck Group (1 mentions)
N acetylglucosamine glcnac, by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Silver nitrate, by Merck Group (1 mentions)
Chitosan from shrimp shells, by Sinopharm (1 mentions)
α amylase, by Merck Group (1 mentions)
Pepsin, by Sinopharm (1 mentions)
3 protocols using chitosanase from streptomyces griseus
1
Characterization of High-Molecular-Weight Chitosan
2
Chitosan-based Antimicrobial Biomaterials
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Chitosan was provided by InFiQus, S.L. (Madrid, Spain), silver nitrate (purity ≥ 99%), lysozyme from chicken hen (EC 3.2.1.17) and chitosanase from Streptomyces griseus (EC 3.2.1.132) were provided by Sigma-Aldrich (St. Louis, MO, USA), dialysis membranes (MWCO-3500, 7000 or 12–14,000 Da) were provided by Medicell International, Ltd. (London, UK). Milli-Q water was used in all experiments.
3
Chitosan Extraction and Modification
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Chitosan from shrimp shells (≥91% deacetylated) and pepsin (EC 3.4.23.1, 3000–3500 units/mg protein) from porcine gastric mucosa (Amresco type A) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Chitosanase from Streptomyces griseus (EC 3.2.1.132) and α-amylase (EC 3.2.1.1) were purchased from Sigma-Aldrich (Shanghai, China) Trading Co, Ltd. All other reagents were of analytical grade and were used without further purification. All solutions were made with redistilled and ion-free water.
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