Sonopuls hd 3100

The same procedure of preparing bacterial protein lysate was used for the comparison of Salmonella Typhimurium protein expression when cultivated in LB or BHI medium using MS and for immunoaffinity chromatography.
Salmonella Typhimurium was grown overnight at 37 °C in LB and BHI medium, as noted above. The culture was then centrifuged (3,500 × g, 10 min), and a cell pellet was washed 3 times in PBS (Dulbecco’s, Lonza, Switzerland). The cell pellet was resuspended in PBS and sonicated (Sonopuls HD 3100, Bandelin, Germany) with zirconia/silica beads (BioSpec Products, USA). The sonicate was centrifuged at 20,000 × g and the supernatant with proteins was taken. The pellet was then resuspended in 8 M urea (Serva, Germany), 0.1 % SDS (Carl Roth, Germany), 2 % Triton X-100 (Serva, Germany) and 25 mM triethylammonium bicarbonate (Sigma-Aldrich, USA) and centrifuged at 20,000 × g again. The supernatant was mixed with the supernatant from the previous step and together used as an antigen for subsequent analysis.

In a typical synthesis,
10 mL of 1-octadecene, 0.5 mL of oleic acid
and 0.5 mL of oleylamine were added to Cs₂CO₃ (0.1 mmol) and PbBr₂ (0.3 mmol) precursor powders. Then,
tip-sonication (SONOPULS HD 3100, BANDELIN) was applied to the mixture
solution at a power of 30 W for 10 min. The as-prepared NC dispersions
were purified without adding any antisolvents by centrifugation at
9000 rpm for 10 min to remove unreacted precursors before redispersing
the NC precipitates in 5 mL of hexane under mild sonication. The obtained
NC dispersions were further centrifuged at 2000 rpm to remove large
NCs. Thus, the obtained CsPbBr₃ NCs solution is ready to
undergo an anion-exchange process to prepare the CsPbBr_xI_3-x NCs.

Particle size distribution was determined by Flow Particles Image Analyzer (FPIA), using a Sysmex FPIA 3000 particle size and shape analyser (Malvern Panalytical, Malvern, UK) and by dynamic light scattering (DLS), using a Zetasizer Nano ZS (Malvern Instruments, Malvern, UK). For FPIA analysis, a sample dispersion of 0.5 mg/ml in Milli-Q water was prepared and probe sonicated on ice for 3 min at 30% amplitude, power 25 W, energy delivered into the sample 0.450 kJ/ml, using a Sonopuls HD3100 homogeniser (Bandelin, Berlin, Germany). For DLS analysis, a sample dispersion in Roswell Park Memorial Institute (RPMI) 1640 medium (0.5 mg/ml), with the addition of 10% foetal bovine serum (FBS), was prepared and sonicated as described.