C57BL/6J female mice (8–12 weeks of age, obtained from Jackson Laboratory #664) were placed on 1% w/w choline diet (Teklad #TD.09041) and given inhibitor or vehicle (water) once daily via gavage. Blood and urine were collected at the indicated days (4, 11, and 14 days after diet start). On day 14, mice were humanely euthanized by anesthesia overdose (>300 mg/kg ketamine + 30 mg/kg xylazine) and blood was collected for analysis of physiologic markers of toxicity. EDTA treated whole-blood from mice treated for 14 days was run on a Siemens Advia 120 Hematology Analyzer for complete blood count (CBC) with differential. Plasma was analyzed for liver (ALT, AST) and kidney function (BUN) using a Roche Cobas C 311 analyzer, and CBC with differential was determined using a Bayer Advia hematology analyzer.
Cobas c 311 analyzer
Manufactured by Bayer
The Cobas C 311 analyzer is a compact, benchtop clinical chemistry analyzer designed for routine in-vitro diagnostic testing. It provides automated analysis of a wide range of clinical chemistry parameters from patient samples. The Cobas C 311 is capable of performing photometric and potentiometric measurements to determine the concentration of analytes in the tested samples.
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2 protocols using cobas c 311 analyzer
1
Choline Diet and Toxicity Markers
2
Choline Diet and Toxicity Markers
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C57BL/6J female mice (8–12 weeks of age, obtained from Jackson Laboratory #664) were placed on 1% w/w choline diet (Teklad #TD.09041) and given inhibitor or vehicle (water) once daily via gavage. Blood and urine were collected at the indicated days (4, 11, and 14 days after diet start). On day 14, mice were humanely euthanized by anesthesia overdose (>300 mg/kg ketamine + 30 mg/kg xylazine) and blood was collected for analysis of physiologic markers of toxicity. EDTA treated whole-blood from mice treated for 14 days was run on a Siemens Advia 120 Hematology Analyzer for complete blood count (CBC) with differential. Plasma was analyzed for liver (ALT, AST) and kidney function (BUN) using a Roche Cobas C 311 analyzer, and CBC with differential was determined using a Bayer Advia hematology analyzer.
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