African monkey kidney cell line (Vero) was purchased from the Korean cell line bank (KCLB, Seoul, Korea). Media for the cell line maintenance (Roswell Park Memorial Institute-1640; RPMI-1640) and serum (fetal bovine serum; FBS) including antibiotics (penicillin, streptomycin) were purchased from Gibco-BRL (Grand Island, NY, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azobis(2-amidinopropane) hydrochloride (AAPH), 5,5-dimethyl-1-pyrolin N-oxide (DMPO), and α-(4-Pyridyl-1-oxide)-N-tert-butylnitrone (POBN) were purchased from Sigma (St. Louis, MO, USA). H2O2 and FeSO4.7H2O used were purchased from Fluka Co. (Buchs, Switzerland). Furthermore, dimethyl sulfoxide (DMSO), 2,7-dichlorofluorescein diacetate (DCF-DA), and 2,2′-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) were obtained from Sigma-Aldrich (St. Louis, MO, USA).
5 5 dimethyl 1 pyrolin n oxide dmpo
Manufactured by Merck Group
Sourced in United States, China
5,5-dimethyl-1-pyrolin N-oxide (DMPO) is a chemical compound used as a spin trap in electron paramagnetic resonance (EPR) spectroscopy. It is utilized to detect and study free radicals and other short-lived reactive species.
Automatically generated - may contain errors
Lab products found in correlation
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions)
2 7 dichlorofluorescein diacetate dcf da, by Merck Group (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
1 diphenyl 2 picrylhydrazyl, by Merck Group (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
2 2 azobis 2 amidinopropane hydrochloride, by Merck Group (1 mentions)
2 2 azino bis 3 ethylbenzthiazoline 6 sulfonic acid abts, by Merck Group (1 mentions)
Sodium persulfate ps na2s2o8, by Merck Group (1 mentions)
2 protocols using 5 5 dimethyl 1 pyrolin n oxide dmpo
1
Vero Cell Line Maintenance Protocol
2
Valorization of Spent Sulfuric Acid
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The black-red and sticky spent sulfuric acid was gathered from the industrial alkylation unit (Shandong, China), which was sealed and stored under −15 °C before use. The compositions of spent sulfuric acid were determined by the titration method, which was 91.34 wt% sulfuric acid, 3.48 wt% water, and 5.18 wt% acid-soluble oil. The characteristics of spent sulfuric acid were listed in Table 1 . The spent sulfuric acid was used as soon as possible because its property changes with time.
Hydrogen peroxide (30 wt% H2O2), ethanol (EtOH, CH3CH2OH), and tert-butyl alcohol (TBA, C4H9OH) were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Sodium persulfate (PS, Na2S2O8) and 5,5-dimethyl-1-pyrolin-N-oxide (DMPO) were supported by Sigma-Aldrich (China). The feedstock for the preparation of OBC in this study was an apricot shell residue, which was collected from Haizhou Water Treatment Materials Co., Ltd (Henan, China). All the solutions were prepared in deionized water.
Hydrogen peroxide (30 wt% H2O2), ethanol (EtOH, CH3CH2OH), and tert-butyl alcohol (TBA, C4H9OH) were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Sodium persulfate (PS, Na2S2O8) and 5,5-dimethyl-1-pyrolin-N-oxide (DMPO) were supported by Sigma-Aldrich (China). The feedstock for the preparation of OBC in this study was an apricot shell residue, which was collected from Haizhou Water Treatment Materials Co., Ltd (Henan, China). All the solutions were prepared in deionized water.
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