Anti flag 2368

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-Flag (2368) is a monoclonal antibody product offered by Cell Signaling Technology. The core function of this antibody is to detect the FLAG tag, which is a commonly used epitope tag for recombinant protein expression and purification.

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Lab products found in correlation

Alexa fluor 568, by Thermo Fisher Scientific (2 mentions) Pertussis toxin, by Enzo Life Sciences (1 mentions) Anti gfp sc 9996, by Santa Cruz Biotechnology (1 mentions) Anti plcγ1 sc 81, by Santa Cruz Biotechnology (1 mentions) Anti gapdh 5174, by Cell Signaling Technology (1 mentions) Anti gst 2622, by Cell Signaling Technology (1 mentions) Anti flag m2 beads a2220, by Merck Group (1 mentions) Cdcl2, by Sangon (1 mentions) Fura 2 am, by Thermo Fisher Scientific (1 mentions) Anti ha sc 7392, by Santa Cruz Biotechnology (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) γ h2ax 2577, by Cell Signaling Technology (1 mentions) Goat anti mouse igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions) Gfp sc 9996, by Santa Cruz Biotechnology (1 mentions) Swine anti rabbit immunoglobulins hrp, by Agilent Technologies (1 mentions) Goat anti rabbit igg h l cross adsorbed secondary antibody, by Thermo Fisher Scientific (1 mentions) Anti ha clone 3f10, by Roche (1 mentions) Anti mcl 1 d35a5, by Cell Signaling Technology (1 mentions) Anti hsp70, by Abcam (1 mentions) Anti usp7, by Abcam (1 mentions)

5 protocols using anti flag 2368

Antibody and Chemical Reagent Protocol

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The anti-TRPC3/6/7 (sc-15058, 1/1,000), anti-GFP (sc-9996, 1/1,000), anti-AT1R (sc-1173, 1/1,000), anti-HA (sc-7392, 1/1,000), anti-PLCγ1 (sc-81, 1/1,000) antibodies were purchased from Santa Cruz. The anti-Flag (2368, 1/1,000), anti-GAPDH (5174, 1/1,000), anti-GST (2622, 1/1,000) antibodies were from Cell Signaling. The anti-HA-tag beads and anti-GFP-tag beads were from Medical & Biological Laboratories Co., Ltd. (Japan). The anti-Flag M2 beads (A2220) were from Sigma. The anti-β-arrestin-1 (A1CT, 1/5,000) and anti-β-arrestin-2 antibodies (A2CT, 1/2,000) were generous gifts from Dr R.J. Lefkowitz (Duke University). The pertussis toxin was from Enzo Life Sciences. The SNX482 was from Abcam. The LaCl₃ and CdCl₂ were purchased from Sangon Biotech (Shanghai) Co. The Fura-2-AM was from Invitrogen. The DMEM medium was from Thermo Scientific. All other chemical or reagents were purchased from Sigma.

Liu C.H., Gong Z., Liang Z.L., Liu Z.X., Yang F., Sun Y.J., Ma M.L., Wang Y.J., Ji C.R., Wang Y.H., Wang M.J., Cui F.A., Lin A., Zheng W.S., He D.F., Qu C.X., Xiao P., Liu C.Y., Thomsen A.R., Joseph Cahill T I.I.I., Kahsai A.W., Yi F., Xiao K.H., Xue T., Zhou Z., Yu X, & Sun J.P. (2017). Arrestin-biased AT1R agonism induces acute catecholamine secretion through TRPC3 coupling. Nature Communications, 8, 14335.

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Comprehensive Antibody Inventory for DNA Damage Research

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Anti-FLAG (#2368) and γH2AX (#2577) antibodies were purchased from Cell Signaling Technology, whereas antibodies for BRCA1 (#sc-6954), H1.4 (#sc-393358), RNF8 (#sc-2714620), and GFP (#sc-9996) were from Santa Cruz Biotechnology. Antibodies against α-tubulin (GTX628802) were obtained from GeneTex. Anti-RPA (ab2175), H1.0 (ab11079), H1.2 (ab17677), H1.3 (ab183736), and H1.4 (ab18208) were purchased from Abcam, whereas anti-H1.1 was from Insight Biotechnology (GTX117055). Finally, anti-RPA pS4+pS8 (A300-245A) antibodies were purchased from Bethyl, whereas anti-H3 antibodies were obtained from Sigma-Aldrich (05-928). Goat anti-mouse immunoglobulins/HRP (#P044701-2) and swine anti-rabbit immunoglobulins/HRP (P039901-2) were purchased from Dako. Goat anti-mouse IgG (H + L) secondary antibody, Alexa Fluor 488 (#A10680) and goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody, and Alexa Fluor 568 (#A11011) were purchased from Thermo Fisher Scientific.

Ozgencil M., Dullovi A., Christiane Higos R.C., Hořejší Z, & Bellelli R. (2023). The linker histone H1–BRCA1 axis is a crucial mediator of replication fork stability. Life Science Alliance, 6(9), e202301933.

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Antibody Panel for Protein Profiling

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The following antibodies were used: anti-K48-linked ubiquitin, clone APU2 and anti-K63-linked ubiquitin, clone APU3 (Millipore); anti-PARP 9542, anti-cAbl 2862, anti-α-tubulin 2156, anti-Mcl-1 D35A5, anti-Flag 2368, (Cell Signaling Technologies); anti-actin clone AC-40 A3853, anti-GAPDH clone G9295, and anti-HA, Clone 3F10 (Roche); anti-ubiquitin, clone 6C1.17 (BD Pharmingen); anti-HSP70, anti-USP7, anti-UCH37, anti-USP24 and anti-USP9x (all rabbit monoclonal) (Abcam); HRP conjugated secondary antibodies (Abcam)

Lawson A.P., Long M.J., Coffey R.T., Qian Y., Weerapana E., El Oualid F, & Hedstrom L. (2015). Naturally occurring isothiocyanates exert anticancer effects by inhibiting deubiquitinating enzymes. Cancer research, 75(23), 5130-5142.

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Protein Immunoprecipitation and Western Blot Analysis

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KGN cells were transfected with the indicated plasmids and specific siRNAs. Twenty-four hours after transfection, cell lysates were prepared for immunoprecipitation with antibody-linked protein G Dynabeads® (Invitrogen) according to the manufacturer's instructions. After incubation, the samples were boiled and subjected to SDS-PAGE and immunoblotting with appropriate antibodies. The membranes were imaged using a ChemiDoc™ XRS+ System Imager (Bio-Rad Laboratories Inc., Hercules, CA, USA). The following antibodies were purchased and used in this study: anti-AMH (AP9940c; Abgent, San Diego, CA, USA), anti-SF-1 (sc-28740; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-GAPDH (sc-25778; Santa Cruz Biotechnology), anti-PARP (sc-74469; Santa Cruz Biotechnology), and control rabbit IgG (sc-2027; Santa Cruz Biotechnology), anti-α-tubulin (LF-PA0146; AbFrontier Seoul, Korea), anti-c-Myc (631206; Clontech), anti-FLAG (2368) (Cell Signaling, Danvers, MA, USA), and anti-FLAG (F1804; Sigma-Aldrich). The polyclonal rabbit FOXL2 antibody was described previously [30 (link)].

Jin H., Won M., Park S.E., Lee S., Park M, & Bae J. (2016). FOXL2 Is an Essential Activator of SF-1-Induced Transcriptional Regulation of Anti-Müllerian Hormone in Human Granulosa Cells. PLoS ONE, 11(7), e0159112.

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Endothelial Cell Alignment Under Shear

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Cells were seeded on plastic-coated cell culture–treated slides with 10 µg/ml bovine fibronectin for ∼24 h, clamped into a 25 × 55 mm parallel plate flow chamber (Frangos et al., 1988 (link)), and sheared at 12 dynes/cm² in complete media for 16 h. Cells were fixed in 3.7% formaldehyde and stained with either anti-Flag (2368; Cell Signaling Technology) or anti–VEcad (C19; Santa Cruz Biotechnology, Inc.), Alexa Fluor 647–phalloidin (Molecular Probes), and Hoechst (Molecular Probes). Slides were mounted in fluoromount-G (SouthernBiotech). Fluorescence microscopy was performed at room temperature with a microscope (80i; Nikon) equipped with 10× (NA 0.45) and 20× (NA 0.75) objective lenses and a CCD camera (Retiga 200R; QImaging). Images were collected throughout the length of the slide with NIS Elements software. Cells were then scored for alignment within ±23° of the axis of shear using ImageJ.

Coon B.G., Baeyens N., Han J., Budatha M., Ross T.D., Fang J.S., Yun S., Thomas J.L, & Schwartz M.A. (2015). Intramembrane binding of VE-cadherin to VEGFR2 and VEGFR3 assembles the endothelial mechanosensory complex. The Journal of Cell Biology, 208(7), 975-986.

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