Rapamycin rapa

Manufactured by Merck Group

Sourced in United States, Sao Tome and Principe

Rapamycin (Rapa) is a macrolide compound with immunosuppressant and antiproliferative properties. It is a natural product derived from the bacterium Streptomyces hygroscopicus.

Automatically generated - may contain errors

Lab products found in correlation

3 methyladenine 3 ma, by Merck Group (9 mentions) Chloroquine cq, by Merck Group (3 mentions) Bafilomycin a1 bafa1, by Merck Group (3 mentions) Beclin 1, by Cell Signaling Technology (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Lc3a b, by Cell Signaling Technology (2 mentions) P mtor, by Cell Signaling Technology (2 mentions) Doxorubicin dox, by Merck Group (2 mentions) Cisplatin, by Merck Group (2 mentions) Bcl 2, by Cell Signaling Technology (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Bafilomycin a1, by Merck Group (2 mentions) Bafa1, by Merck Group (2 mentions) P erk, by Cell Signaling Technology (1 mentions) Rhodamine phalloidin, by Cytoskeleton (1 mentions) Xanthine oxidase, by Merck Group (1 mentions) 4 pba, by Merck Group (1 mentions) Mitotempo, by Merck Group (1 mentions) Grp78, by Abcam (1 mentions) Gsk 3β, by Abcam (1 mentions)

Close spelling variants of this product

Rapamycin (1486 citations)

37 protocols using rapamycin rapa

Modulating Cellular Autophagy Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cellular autophagy was induced by nutrient deprivation through incubation in EBSS (Sigma, St. Louis, MO, USA). Autophagy inhibition was achieved by treating cells with 10 μM of CQ (Sigma), a lysosomal inhibitor. Rapamycin (RAPA, a specific mTOR inhibitor, Sigma) was used at 0.1 nM concentration to block mTOR activity.

Xue Y., Han H., Wu L., Pan B., Dong B., Yin C.C., Tian Z., Liu X., Yang Y., Zhang H., Chen Y, & Chen J. (2017). iASPP facilitates tumor growth by promoting mTOR-dependent autophagy in human non-small-cell lung cancer. Cell Death & Disease, 8(10), e3150-.

+ Open protocol

+ Expand

Investigating Cellular Mechanisms in Stress Response

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pharmacological agents used: angiotensin II (Ang II), chloroquine (CQ), Xanthine oxidase (XO), and rapamycin (Rapa) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Rhodamine phalloidin was acquired from Cytoskeleton (Denver, CO, United States). Mito-TEMPO and 4-PBA were obtained from Sigma-Aldrich (Sigma, St. Louis, MO, United States). Antibodies against LC3A/B, ATG5, p62, mTOR, p-mTOR, ATG5, ATG7, Beclin 1, PERK, XBP1, CHOP, β-actin, and GAPDH were purchased from Cell Signaling Technology (Boston, MA, United States). GRP78, GSK3β, p-GSK3β (Ser9), β-TrcP, and NRF2 were purchased from Abcam (Cambridge, MA, United States). NOX2, NOX4, and HO-1 were provided by Proteintech (Wuhan, China).

Han D., Wang F., Wang B., Qiao Z., Cui X., Zhang Y., Jiang Q., Liu M., Shangguan J., Zheng X., Bai Y., Du C, & Shen D. (2022). A Novel Compound, Tanshinol Borneol Ester, Ameliorates Pressure Overload-Induced Cardiac Hypertrophy by Inhibiting Oxidative Stress via the mTOR/β-TrCP/NRF2 Pathway. Frontiers in Pharmacology, 13, 830763.

+ Open protocol

+ Expand

Iron Depletion and Lipid Modulation in Yeast

Check if the same lab product or an alternative is used in the 5 most similar protocols

Yeasts were grown at 30 °C in SD medium (2% glucose, 0.67% yeast nitrogen base that lacked the corresponding amino acids for plasmid maintenance) plus amino acids [54 (link)]. For iron depletion conditions (SD-Fe), SD medium was used with a yeast nitrogen base that is free of iron plus the addition of 80 µM of 4,7-diphenyl-1,10-phenanthrolinedisulfonic acid (BPS) (Sigma, 146617, St. Louis, MO, USA).
We present a list of reagents detailing the final concentration in culture media and from which company they were purchased: N-acetylcysteine (NAC) 5 mM (Sigma, A9165); rapamycin (Rapa) 200 ng/mL (Sigma, R0395); cycloheximide (CHX) 150 mg/mL (Sigma, C4859); DAPI 2 mg/mL (Sigma, D9541); (N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenylhexatrienyl)) pyridinium dibromide (FM4-64) 30 µg/µL (Invitrogen, T-3166); Myriocin (Myr) 2 mM (Sigma, M1177); D-erythro-Dihydrosphingosine (DHS) 20 µM (Sigma, D3314); Aureobasidin A (Aur) 250 ng/mL (MedChem Express, HY-P1975, South Brunswick Township, NJ, USA). Cell cultures were exponentially grown at 600 nm [OD₆₀₀] of 0.6 or longer times as indicated. Iron was added as ammonium iron (III) sulfate hexacahydrate [NH₄Fe(SO₄)₂•6H₂O] (+Fe) (Sigma, F1543) at a final concentration of 10 mM.

Montellà-Manuel S., Pujol-Carrion N, & de la Torre-Ruiz M.A. (2023). Aft1 Nuclear Localization and Transcriptional Response to Iron Starvation Rely upon TORC2/Ypk1 Signaling and Sphingolipid Biosynthesis. International Journal of Molecular Sciences, 24(3), 2438.

+ Open protocol

+ Expand

Seriniquinone and Analogues Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Unless noted, all compounds were prepared as stocks in DMSO (Synth, Diadema, SP, Brazil). Seriniquinone (SQ1) and its synthetic analogues SQ2, SQ3, SQ4, and SQ5 (Figure 1A) were obtained as described in [2 (link)] and were used with ≥98% purity. All other compounds were obtained from commercial sources, as follows: doxorubicin (DOX; Sigma-Aldrich, St. Louis, MO, USA), rapamycin (RAPA; Sigma-Aldrich, St. Louis, MO, USA), bafilomycin A1 (Selleck Chemicals, Houston, TX, USA), and Z-VAD-FMK (Cayman Chemical Company, Ann Arbor, MI, USA).

Hirata A.S., Rezende-Teixeira P., Machado-Neto J.A., Jimenez P.C., Clair J.J., Fenical W, & Costa-Lotufo L.V. (2021). Seriniquinones as Therapeutic Leads for Treatment of BRAF and NRAS Mutant Melanomas. Molecules, 26(23), 7362.

+ Open protocol

+ Expand

Osteogenic Differentiation and Autophagy Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Alizarin red S, β-glycerophosphate disodium salt hydrate, dexamethasone, D-glucose, glucose oxidase, rapamycin (RAPA), and L-ascorbic acid were obtained from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and interleukin-1-beta (IL-1β) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Bone morphogenetic protein-2 (BMP-2), BMP-7, runt-related transcription factor-2 (RUNX-2), and β-actin were obtained from Bioworld Technology (Louis Park, MN, USA). Tumor necrosis factor-alpha (TNF-α) and light chain 3 (LC3I/II) were obtained from Cell Signaling Technologies (Beverly, MA, USA). Cementum protein 1 (CEMP-1) was purchased from LifeSpan BioSciences (Seattle, WA, USA). Autophagy related 5 (ATG5) was obtained from Novus Biologicals (Littleton, CO, USA). Beclin-1 was purchased from Bethyl Laboratories (Montgomery, TX, USA).

Kim J.E., Kim T.G., Lee Y.H, & Yi H.K. (2020). Phelligridin D maintains the function of periodontal ligament cells through autophagy in glucose-induced oxidative stress. Journal of Periodontal & Implant Science, 50(5), 291-302.

+ Open protocol

+ Expand

Autophagy Modulation in U-251 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

U-251 cells were grown to ~70% confluency for various experiments. Cells were treated with various concentrations of the autophagy inhibitor Bafilomycin A1 (BafA1) (Sigma Aldrich, St. Louis, MO, U.S.A.) or the autophagy inducer Rapamycin (Rapa) (Sigma Aldrich) for various periods of time.

Sher A.A., Gao A, & Coombs K.M. (2020). Autophagy Modulators Profoundly Alter the Astrocyte Cellular Proteome. Cells, 9(4), 805.

+ Open protocol

+ Expand

Lipid Modulators in Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Palmitic acid (PA), bafilomycin A1 (BA), ammonium chloride (NH₄Cl), JAK2 specific inhibitor AG490, rapamycin (RAPA) and free fatty acid (FFA) free-bovine serum albumin (BSA) and lipopolysaccaride (LPS) were purchased from Sigma Aldrich (St. Louis, Missouri, USA). Thioglycollate was from Difco (Detroit, MI, USA). Interferon γ (INF-γ) and interleukin-4 (IL-4) were purchased from R&D systems. All the antibodies used in the experiments were purchased from Cell signaling Technology Inc. (Danvers, MA, USA), R&D Systems (Minneapolis, MN, USA) or Santa Cruz Biotechnology Inc. (Dallas, Texas, U.S.A.). BMS309403 was synthesized as previously described67 (link).

Hoo R.L., Shu L., Cheng K.K., Wu X., Liao B., Wu D., Zhou Z, & Xu A. (2017). Adipocyte Fatty Acid Binding Protein Potentiates Toxic Lipids-Induced Endoplasmic Reticulum Stress in Macrophages via Inhibition of Janus Kinase 2-dependent Autophagy. Scientific Reports, 7, 40657.

+ Open protocol

+ Expand

Rapamycin and Tacrolimus for Transplant

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rapamycin (RAPA; Sigma) solution was prepared by dissolving Rapamycin (RAPA) 1 mg in 4 mL of 0.2% Carboxyl Methylcellulose by sonication and given at 0.1 mg/kg/d, intraperitoneal to recipient mice from the day prior to transplant (d -1) to POD8. Subcutaneous tacrolimus (TAC, Astellas Pharma US, Inc.), 1 mg/kg daily, was administered from day -1 to POD8 (heart), or day -1 to POD8 (liver).

Schneiderman J., Qiu L., Yeap X.Y., Kang X., Zheng F., Ye J., Xie Y., Wang J.J., Sambandam Y., Mathew J., Li L., Leventhal J., Edelson R.L, & Zhang Z.J. (2022). Pre-transplant infusion of donor leukocytes treated with extracorporeal photochemotherapy induces immune hypo-responsiveness and long-term allograft survival in murine models. Scientific Reports, 12, 7298.

+ Open protocol

+ Expand

Podocyte and Mesangial Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

MPC5 mouse glomerular podocytes were purchased from BeNa Culture Collection, and cultured with 10% FBS (Excell Bio, South America, FSP500) and low-glucose DMEM(Sigma-Aldrich, 6046). SV40 MES 13 mouse glomerular mesangial cells were purchased from the typical culture preservation committee cell bank of the Chinese Academy of Sciences, and cultured with 10% FBS (Excell Bio, South America, FSP500) and high-glucose DMEM (Sigma-Aldrich, 6429). The CO₂ concentration of the cell culture box was maintained at 5% and the temperature was maintained at 37 °C. Chloroquine (CQ) and rapamycin (RAPA) were purchased from Sigma-Aldrich (C6628, V900930). 10 mM CQ and 25 mg/mL rapamycin were both dissolved in ultra-pure water. CQ was added to the petri dish so that the final concentration was 50 μM, and incubated for 16 h as an autophagy inhibitor. Rapamycin was added to the petri dish at a final concentration of 15 μM and incubated for 2 h as an autophagy activator.

Geng M.Y., Wang L., Song Y.Y., Gu J., Hu X., Yuan C., Yang M., Pei W.J., Zhang Y, & Gao J.L. (2021). Sidt2 is a key protein in the autophagy-lysosomal degradation pathway and is essential for the maintenance of kidney structure and filtration function. Cell Death & Disease, 13(1), 7.

+ Open protocol

+ Expand

Autoimmune Disease Modeling in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57Bl/6J (B6) were originally obtained from the Jackson Laboratory. B6.NZM-Sle1^NZM2410/AegSle2^NZM2410/AegSle3^NZM2410/Aeg/LmoJ (TC) congenic mice have been previously described (17 (link)). All mice were bred and maintained at the University of Florida in specific pathogen-free conditions. For in vivo treatment, 2DG (Sigma, 6 mg/ml) was dispensed in drinking water for the entire duration of each experiment. Age-matched control mice received plain drinking water. DON (Sigma, 1.6 mg/kg) was injected intra-peritoneally every other day for 2 weeks. Rapamycin (RAPA, Sigma 8 ug/kg) was injected daily intra-peritoneally. Control mice received PBS injections. Only female mice were used at the age indicated for each experiment, in which pre-autoimmune and autoimmune stages were defined relative to the production of anti-dsDNA IgG, which starts between 4 and 5 months of age in that strain. All protocols approved by the Institutional Animal Care and Use Committees of the University of Florida.

Choi S.C., Li W., Zhang X., Kanda N., Zeumer-Spataro L., Teng X, & Morel L. (2022). Pharmacologically inferred glycolysis and glutaminolysis requirement of B cells in lupus-prone mice. Journal of immunology (Baltimore, Md. : 1950), 208(9), 2098-2108.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!