The concentrations of FVII (EM1041, FineTest), sP-selectin (HD32526, DIBCO), CRP (MCRP00, R&D), TNF-α (MTA00B, R&D), IL-6 (M6000B, R&D), D-dimer (CSBE13584m, CUSABIO), tPA (SEA525Mu, Cloud clone Corp) and PAI-1 (SEA532Mu, Cloud clone Corp) were measured using ELISA kits according to the manufacturer`s instructions. The activity of, tPA (PA90, Oxford) and PAI-1 (IMSPAI1KTA, Innovative Research) in mouse serum was also measured using ELISA kits. For the detection of other indicators, after 5 days of treatment, blood was collected from the in each group mice via the venous sinus and was tested by ELISA.
Mcrp00
Manufactured by R&D Systems
Sourced in United Kingdom, United States
The MCRP00 is a multi-channel reagent dispenser designed for precision liquid handling in a variety of laboratory settings. It features adjustable dispensing volumes, multiple channels, and accurate liquid delivery.
Automatically generated - may contain errors
Lab products found in correlation
Mta00b, by R&D Systems (2 mentions)
Csbe13584m, by Cusabio (1 mentions)
Quantikine elisa kits, by Abcam (1 mentions)
X rad 320 platform, by Precision X-Ray (1 mentions)
Ab100688, by Abcam (1 mentions)
Hybri max, by Merck Group (1 mentions)
Total cholesterol, by Randox (1 mentions)
Ezrmi 13k, by Merck Group (1 mentions)
Kge014, by R&D Systems (1 mentions)
6 protocols using mcrp00
1
Multimarker Profiling of Mouse Serum
2
Investigating Cardiotoxicity Models in Mice
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Mice were randomly assigned to each group. To establish a Dox-induced cardiotoxicity model, mice were intraperitoneally injected with Dox (4 mg/kg, 6 times/2 weeks); controls were injected with the vehicle. To establish a radiation-induced cardiotoxicity model, the thoracic cavities of mice were irradiated with 16 Gy (BALB/c nude mice) using the X-RAD 320 platform (Precision X-ray). Ab417 (10 mg/kg, 6 times/2 weeks) was injected intravenously into BALB/c nude mice 1 h before the start of Dox or radiation treatment. Control mice were administered an equivalent dose of an isotype control antibody (IgG). The serum concentrations of CRP (catalogue no. MCRP00; R&D Systems), E-selectin, and ICAM-1 (catalogue no. ab171182 and ab100688; Abcam) were measured using mouse Quantikine ELISA kits.
3
Plasma Cholesterol and CRP Measurement
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Total plasma cholesterol and C-reactive protein (CRP) were measured with commercially available ELISA kits (Total cholesterol, Randox, Crumlin, UK and MCRP00, R&D systems, Minneapolis, MN, USA respectively).
Red blood cells in EDTA whole blood were lysed (red blood cell lysing buffer Hybri-Max, Sigma, St. Louis, MO, USA), followed by the labeling of the remaining leukocytes (monocytes, neutrophils and dendritic, natural killer (NK), natural killer T (NKT) and T-cells). Next, labeled leukocytes were analyzed by flow cytometry as described previously [10 (link)].
Red blood cells in EDTA whole blood were lysed (red blood cell lysing buffer Hybri-Max, Sigma, St. Louis, MO, USA), followed by the labeling of the remaining leukocytes (monocytes, neutrophils and dendritic, natural killer (NK), natural killer T (NKT) and T-cells). Next, labeled leukocytes were analyzed by flow cytometry as described previously [10 (link)].
4
Metabolic Profile Assessment in Mice
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Six mice in each group were fasted overnight and then were anesthetized. Mice were anesthetized (30 mg/kg of pentobarbital by intraperitoneal injection). Blood (3 mL) was collected through the abdominal aorta, and serum was centrifuged and stored at −80°C for further use. BG (Blood glucose), insulin (Ins), TG (triacylglycerol), TC (total cholesterol), LDL-C (low density lipoprotein cholesterol), and HDL-C (high density lipoprotein cholesterol), FFA (free fatty acid), were measured with an automatic biochemical analyzer. ELISA examination was carried out to detect serum TNF-α (tumor necrosis factor alpha) (MTA00B; R&D Systems, Minneapolis, MN), CRP (C-reactive protein) (MCRP00, R&D Systems, Minneapolis, MN), and Insulin (EZRMI-13K, Millipore, Billerica, MA) based on the instruction of the manufacturer.
5
Inflammatory Cytokine Profiling in Mouse Model
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Mouse blood samples were obtained after six weeks of PM or vehicle exposure. Blood serum was obtained by centrifuging these blood samples for 20 min at 300 g. The pro-inflammatory cytokines tumor necrosis factor (TNF)-α (Cat#430904), interleukin (IL)-1β (Cat#432604), and IL-6 (Cat#431304), as well as anti-inflammatory cytokine IL-10 (Cat#431414), were measured with an ELISA kit (BioLegend, San Diego, CA, USA). Serum C-reactive protein (CRP, R&D System, Cat#MCRP00), and estradiol (R&D systems, Cat#KGE014) were quantitatively determined using ELISA kits as per the manufacturer’s protocols.
6
Plasma Cholesterol and CRP Analysis
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Analysis of total plasma cholesterol was performed by using a commercially available kit (Randox, Crumlin, UK). Plasma lipoprotein profiles were determined on pooled samples (3 per group) by fast protein liquid chromatography on a Superose 6 column. Plasma C-reactive protein (CRP) was measured using ELISA (MCRP00, R&D systems, Minneapolis, USA).
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