G6p assay kit

Manufactured by Abcam

Sourced in United States

The G6P Assay Kit is a quantitative colorimetric assay designed to measure glucose-6-phosphate (G6P) levels in various sample types, including biological fluids, cell lysates, and tissue homogenates. The kit utilizes an enzymatic reaction to produce a colored product, which is then measured spectrophotometrically.

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2 protocols using g6p assay kit

Glucose Metabolism Analysis in CRC Cells

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Following transfection, the CRC cells (1×10⁶ cells/well) were incubated for 24 h. Glucose levels in the supernatants of cultured cells and cell lysates were measured using the Glucose Colorimetric Assay Kit (BioVision, Milpitas, USA) and the levels of lactate in the supernatants of cultured cells were quantified by an automatic analyzer. The levels of intracellular G6P in cell lysates were analyzed using a G6P Assay kit (BioVision, Milpitas, USA). In addition, the levels of intracellular G6PD enzymatic activity and NADPH in cell lysates were determined using G6PD Assay Kit (ab102529, Abcam, UK) and Amplite TM Colorimetric NADP/NADPH Ratio Assay Kit (ab65349, Abcam, UK), respectively.

Xia L., Lin J., Peng M., Jiang X., Peng Q., Cui S., Zhang W., Li S., Wang J., Oyang L., Tan S., Hu Z., Wu N., Tang Y., Luo X., Ren Z., Shi Y., Liao Q, & Zhou Y. (2024). Diallyl disulfide induces DNA damage and growth inhibition in colorectal cancer cells by promoting POU2F1 ubiquitination. International Journal of Biological Sciences, 20(4), 1125-1141.

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Glucose Deprivation Affects G-6-P Levels

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Briefly, 5 × 10⁶ cryopreserved cells were rested in the supplemented RPMI-1640 medium with or without glucose (glucose-limiting media) for 4 h. The cells were then pelleted, washed once in ice-cold 1× PBS, and resuspended in 50 μl of ice-cold 1× PBS. Cells were sonicated for 30 min on ‘high setting’ (Soniciean Pty Ltd, Thebarton, South Australia, Australia). Cell lysates were centrifuged at 10 000 r.p.m. at 4°C for 5 min and glucose-6-phosphate (G-6-P) levels were determined in the supernatant using a G-6-P assay kit (Biovision, San Francisco, California, USA).

Palmer C.S., Ostrowski M., Gouillou M., Tsai L., Yu D., Zhou J., Henstridge D.C., Maisa A., Hearps A.C., Lewin S.R., Landay A., Jaworowski A., McCune J.M, & Crowe S.M. (2014). Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection. AIDS (London, England), 28(3), 297-309.

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