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2 protocols using anti pstair

1

Comprehensive Antibody Characterization Protocol

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Primary antibodies used in the current study were as follows: anti-XCAP-C (in-house identifier: AfR8) [33 (link)]; anti-CAP-E (in-house identifier: AfR9) [33 (link)]; anti-XCAP-D2 (in-house identifier: AfR16) [25 (link)]; anti-XCAP-H (in-house identifier: AfR20) [25 (link)]; anti-pT1314-XCAP-D2 (in-house identifier: AfR42-P) [25 (link)]; anti-pT1348-XCAP-D2 (in-house identifier: AfR44-P) [25 (link)];anti-pT1353-XCAP-D2 (in-house identifier: AfR46-P) [25 (link)]; anti-hCAP-D2 (in-house identifier: AfR51-5L) [34 (link)]; anti-pT1339-hCAP-D2 (in-house identifier: AfR173-4P) [32 (link)]; anti-pT1384-hCAP-D2 (in-house identifier: AfR175-4P) [32 (link)]; anti-FLAG M2 (Sigma, F-1804 [RRID: AB_262044]); anti-PSTAIR (Abcam, ab10345, [RRID: AB_297080]); anti-pT161-Cdk1 (Cell Signaling Technology, 9114, [RRID: AB_2074652]). Secondary antibodies used in the current study were as follows: horseradish peroxidase-conjugated anti-rabbit IgG (Vector Laboratories, PI-1000 [RRID: AB_2336198]); horseradish peroxidase-conjugated anti-mouse IgG (Vector Laboratories, PI-2000 [RRID: AB_2336198]); IRDye 680RD goat anti-rabbit IgG (LI-COR Biosciences, 925–68071 [RRID: AB_2721181]); IRDye 800CW goat anti-mouse IgG (LI-COR Biosciences, 925–32210[RRID: AB_2687825]).
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2

Antibodies for Cell Cycle Analysis

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Rabbit polyclonal affinity–purified anti-Cyclin Ba/b (acetyl-NRDLNIQESGPVKAVVNAC-amide; acetyl-CLEFLRRFSRVAEETIDPKEY-amide), anti-CDK1b (acety1-CPDFTKPSTLNVNTSLNEMDM-amide), anti-CDK1c (acetyl-MRKRNIDRPPTQDLNNYC–amide), ant-CDK1d (acety1-MIKAKSGTQDLSNYKRC-amide), and anti-lamin1 (acetyl-QSPISLPPLSGSTC-amide) were from 21st Century Biochemicals (Marlboro, MA). Other antibodies: anti-PSTAIR (Abcam, ab10345), anti-ATPB (Abcam, ab37922), anti-histone 3 (Abcam, ab10799), anti-H3-S28 (Abcam, ab10543), anti-GFP (AMS biotechnology, M30939-2). Secondary antibodies for immunofluorescence: goat anti-rabbit IgG (H+L) Alexa Fluor 568 (Thermofisher, A-11011); and for western blots: goat anti-rabbit IgG (H+L) superclonal™ secondary antibody, HRP (Thermofisher, A27033), Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson ImmunoResearch, 115-035-003).
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