Seven females of E. scrobiculatus and E. brandti were fixed in 70% ethanol. The rostrum with mouthparts was first removed from the body with fine forceps and needle. Then the samples were fixed in 2.5% glutaraldehyde for two hours and washed with phosphate buffer at pH7.8 three times for 15 min. The samples were then dehydrated in a graded alcohol series of 30%, 50%, 70%, 80%, 90%, 95%, 100%, in each case for 15 min, and with one repeat at 100% ethanol. The 100% ethanol was then replaced with 100% tertbutanol in a graded series (3:1, 2:2, and 1:3, by volume) for 15 min at each step. Specimens were stored in 100% tertbutanol for 30 min. The specimens were critical point-dried, mounted on stubs with double-side sticky tape, and were sputter-coated with gold by an E-1010 sputter ion instrument (Hitachi, Tokyo, Japan) before examination with an S-3400 N (Hitachi) scanning electron microscope at an accelerating voltage of 0.5–30 KV.
E 1010 sputter ion instrument
Manufactured by Hitachi
Sourced in Japan
The E-1010 sputter ion instrument is a laboratory equipment designed for sputter deposition. It is used to deposit thin films of materials onto substrates through the process of sputtering. The instrument allows for controlled deposition of various materials, such as metals, alloys, and compounds, onto a variety of substrate surfaces.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using e 1010 sputter ion instrument
1
Preparation of Ethanol-Fixed Escarpia Specimens for SEM
2
Comparative Morphology of Eurhynchius Weevils
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Twenty specimens of E. scrobiculatus females and twenty specimens of E. brandti females were put into a plastic bottle filled with 70% ethanol. Each weevil was taken out of the bottle one by one, and its head (with rostrum) and body were separated with tweezers and dissecting scissors. The dissected head was put into ultrapure water for ultrasonic cleaning, and the water was changed every 5 min for a total of 15 min. The cleaned heads were dehydrated with 30%, 50%, 70%, 80%, 90%, 95%, and 100% alcohol gradients for 15 min, which was repeated once in 100% ethanol. The mandibles, maxillae, and labium of the two weevils were dissected under a microscope (DM2500, Leica), and at the same time, the samples were pasted on the sample stage according to the required shooting angle with conductive glue. The stage with the samples attached was placed in a plastic box filled with silica gel desiccant and dried at room temperature. All samples were coated with a film of gold-palladium in an E-1010 sputter ion instrument (Hitachi, Tokyo, Japan). Then, the observation and photographs were taken under an S-3400N (Hitachi) scanning electron microscope at an accelerating voltage of 0.5–30 KV.
3
Scanning Electron Microscopy of Euphausia Crustaceans
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seven females of E. scrobiculatus and E. brandti were xed in 70% ethanol. The rostrum with mouthparts was rst removed from the body with ne forceps and needle. Then the samples were xed in 2.5% glutaraldehyde for two hours and washed with phosphate buffer at pH7.8 three times for 15 min. The samples were then dehydrated in a graded alcohol series of 30%,50% 70% 80% 90% 95% 100%, in each case for 15 min, and with one repeat at 100% ethanol. The 100% ethanol was then replaced with 100% tertbutanol in a graded series (3:1, 2:2, and 1:3, by volume) for 15 min at each step. Specimens were stored in100% tertbutanol for 30 min. The specimens were critical point-dried, mounted on stubs with double-side sticky tape, and were sputter-coated with gold by an E-1010 sputter ion instrument (Hitachi, Tokyo, Japan) before examination with an S-3400N (Hitachi) scanning electron microscope at an accelerating voltage of 0.5-30 KV.
4
Structural Changes in Curdlan Gel Induced by CBM6E-CBM6
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Microstructural alterations in the curdlan gel following incubation with CBM6E-CBM6 were examined using SEM. Curdlan high-set gel was prepared by preheating it at 80 °C for 4 h. In the experimental group, 800 μL of a 20 mg/mL curdlan gel was incubated with 200 μL of either a 0.5 mg/mL CBM6E-CBM6 or CBM6E-CBM6-T376A mutant protein solution at 18 °C for 9 h. Conversely, the control group of pure curdlan gel underwent incubation with only a buffer (20 mM Tris–HCl, pH 8.0, 200 mM NaCl).
The incubated samples were snap-frozen and placed in an ultra-low temperature freezer at − 80 °C for 2 h, and then freeze-dried with a freeze dryer at − 40 °C for 12 h to obtain SEM samples. Before observation, the gel with gold was sprayed using HITACHI E-1010 ion sputter instrument.
The incubated samples were snap-frozen and placed in an ultra-low temperature freezer at − 80 °C for 2 h, and then freeze-dried with a freeze dryer at − 40 °C for 12 h to obtain SEM samples. Before observation, the gel with gold was sprayed using HITACHI E-1010 ion sputter instrument.
5
Sample Preparation for SEM Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After fixation for more than 2 h, the samples were washed three times with PBS (pH 7.4), prior to sequential dehydration using 50, 70, 80, 90, and 100% ethanol. The ethanol was then replaced with tertiary butyl alcohol before critical point drying using a Hitachi freeze dryer (ES-2030, Hitachi, Tokyo, Japan). The dried samples were then sputter coated with approximately 10 nm of Au/Pd using a Hitachi E-1010 Ion sputter instrument (Hitachi, Tokyo, Japan). SEM imaging was performed on a Hitachi S-3000 N microscope (Hitachi, Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!