Apoptotic rates were assessed with flow cytometry using the Annexin V–fluorescein isothiocyanate/and 7AAD (BD Pharmingen, San Diego, CA, USA). Samples were prepared according to manufacturer’s instructions. Flow cytometry analysis was performed using a FACS-Calibur cytometer using Cell Quest computer software (Becton Dickinson, Heidelberg, Germany).
Cell quest computer software
Manufactured by BD
Sourced in Germany
Cell Quest is a computer software designed for flow cytometry data analysis. It provides tools for processing and visualizing data collected from flow cytometry experiments.
Automatically generated - may contain errors
4 protocols using cell quest computer software
1
Apoptosis Analysis by Flow Cytometry
2
Apoptosis Quantification in Breast Cancer
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Examined breast cancer cells (MDA-MB-231) were stained with annexin V–fluorescein isothiocyanate/and 7AAD (BD Pharmingen, San Diego, CA, USA), and the samples were prepared following the specifications given by manufacturer. The population of apoptotic cells was analyzed by flow cytometry using a FACS Calibur cytometer and Cell Quest computer software (Becton Dickinson, Heidelberg, Germany).
3
Annexin V-FITC Apoptosis Assay
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Apoptotic rates were assessed with flow cytometry using the Annexin V fluorescein isothiocyanate/propidium iodide kit (BD Pharmingen, San Diego, CA, USA). Samples were prepared according to the manufacturer’s instructions. Flow cytometry analysis was performed using an FACS Calibur cytometer using Cell Quest computer software (Becton Dickinson, Heidelberg, Germany).
4
Flow Cytometric Analysis of Cell Cycle
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Impact of the novel ruthenium(II)-arene complexes (C1-C4), starting ruthenium(II)-arenes (C5, C6) and ligands (L1, L2), as well as reference compound cisplatin (cisdiamminedichloroplatinum(II), CDDP) on viability of cultured cells, was determined using the colorimetric 3-(4. Flow cytometric analysis of the cell cycle phase distribution was performed in fixed HCC1937 cells after staining with propidium iodide (PI), as previously described [71, 72] (link).
Briefly, after fixation, cells were washed with cold PBS, incubated with 100 µg/mL of ribonuclease A (RNaseA; 1 mg/mL in PBS) for 30 min, and stained with 50 µg/mL of PI (400 µg/mL in PBS) in the dark, immediately before analysis. 10000 cells were collected for each sample and the cell cycle phase distribution was analyzed using a fluorescence-activated cell sorting (FACS) BD Calibur flow cytometer and Cell Quest computer software (Becton Dickinson, Heidelberg Germany).
Briefly, after fixation, cells were washed with cold PBS, incubated with 100 µg/mL of ribonuclease A (RNaseA; 1 mg/mL in PBS) for 30 min, and stained with 50 µg/mL of PI (400 µg/mL in PBS) in the dark, immediately before analysis. 10000 cells were collected for each sample and the cell cycle phase distribution was analyzed using a fluorescence-activated cell sorting (FACS) BD Calibur flow cytometer and Cell Quest computer software (Becton Dickinson, Heidelberg Germany).
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