Liquid chromatography–MS grade acetonitrile (ACN), methanol, and water were obtained from Burdick & Jackson (Muskegon, MI). Reagents for protein chemistry, including SDS, ammonium bicarbonate, triethylammonium bicarbonate (TEAB), iodoacetamide, DTT, sequencing-grade endoproteinase Lys-C, and formic acid (FA) were purchased from Sigma-Aldrich (St. Louis, MO). Sequencing-grade trypsin was purchased from Promega (Madison, WI). Glycerol-free Peptide:N-glycosidase F was purchased from New England BioLabs (Ipswich, MA).
Glycerol free peptide n glycosidase f
Manufactured by New England Biolabs
Glycerol-free Peptide:N-glycosidase F is an enzyme that cleaves the linkage between asparagine residues and carbohydrates in glycoproteins.
Automatically generated - may contain errors
Lab products found in correlation
Triethylammonium bicarbonate, by Merck Group (1 mentions)
Sequencing grade trypsin, by Promega (1 mentions)
Formic acid, by Merck Group (1 mentions)
Sequencing grade endoproteinase lys c, by Merck Group (1 mentions)
Ms grade acetonitrile, by Avantor (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Iodoacetamide, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Ammonium bicarbonate, by Merck Group (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Hepes, by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
Acetonitrile, by Honeywell (1 mentions)
2 protocols using glycerol free peptide n glycosidase f
1
Protein Sample Preparation for Mass Spectrometry
2
PNGase F-Mediated N-Glycan Profiling
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Sucrose and HEPES were acquired from Sigma-Aldrich, and protease inhibitor cocktail V was purchased from Millipore-Sigma. Glycerol-free Peptide N-glycosidase F (PNGase F) was obtained from New England Biolabs. Acetonitrile was purchased from Honeywell, and formic acid was purchased from Fisher Scientific. Porous graphitized carbon (PGC) 96-well plates were obtained from Glygen Corp. MilliQ water was used throughout the study.
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