Tissue tack positively charged microscope slides

Trifluoroacetic acid, α-cyano-4-hydroxycinnamic acid (CHCA), sodium hydroxide (NaOH), 1-hydroxybenzotriazole hydrate (HOBt), and trypsin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Ammonium bicarbonate, HPLC grade methanol, ethanol, acetonitrile, xylene and water were obtained from Fisher Scientific (Pittsburgh, PA, USA). 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was obtained from Oakwood Chemical (West Columbia, SC, USA). Tissue Tack positively charged microscope slides were purchased from Polysciences, Inc. (Warrington, PA, USA). Citraconic anhydride for antigen retrieval was from Thermo Scientific (Bellefonte, PA, USA). Recombinant Peptide N-Glycosidase F (PNGaseF) from Flavobacteriummeningosepticum was expressed and purified as previously described [20 (link)], and is available commercially as PNGase F Prime™ from Bulldog Bio (Portsmouth, NH, USA).