Alphaview system

Manufactured by Cell Biosciences

Sourced in United States

The AlphaView system is a laboratory instrument designed for the analysis of biomolecular interactions and protein structure. It utilizes advanced imaging and detection technologies to provide researchers with high-quality data on a range of biological samples.

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Lab products found in correlation

3 protocols using alphaview system

Western Blot Analysis of Cell Signaling

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Cells and clinical tissues were lysed on ice in lysis buffer containing freshly added protease inhibitor cocktail (Roche Diagnostics, Branchburg, NJ, USA). The protein extracts (15 µg) were separated using SDS-PAGE and transferred into polyvinylidene difluoride (PVDF) membranes (EMD Millipore, Billerica, MA, USA). The appropriate primary antibodies were used after the membranes were blocked in 5% fat-free milk. The primary antibodies included the following: anti-MSI1 (1:1,000, cat. no. sc-98845; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-p21 (1:500, cat. no. sc-397; Santa Cruz Biotechnology, Inc.), anti-p27 (1:500, cat. no. sc-397; Santa Cruz Biotechnology, Inc.) and anti-β-actin (1:500, cat. no. sc-47778; Santa Cruz Biotechnology, Inc.). Blots were incubated with a secondary antibody coupled to horseradish peroxidase (Thermo Fisher Scientific Inc.), and visualized on X-ray film. Relative quantitation was measured using the AlphaView system (Cell Biosciences, Santa Clara, CA, USA).

Niu J., Zhao X., Liu Q, & Yang J. (2017). Knockdown of MSI1 inhibited the cell proliferation of human osteosarcoma cells by targeting p21 and p27. Oncology Letters, 14(5), 5271-5278.

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Myocardial Protein Analysis via Western Blot

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Myocardial tissues were grounded and homogenized with lysis solution. After sonication, the lysates were centrifuged, and the proteins were separated using electrophoresis and transformation to polyvinylidene fluoride (PVDF) membranes. After being blocked with 5% skim milk in Tris-buffered saline (TBS) for 2 hours at room temperature, the membrane was incubated with primary antibodies against BDNF rabbit antibody (1/1000 dilution; Abcam, Cambridge, UK), Bax, Bcl-2, caspase-3, cleaved caspase-3 rabbit antibody (1/1000 dilution; Cell signaling Tecnology, USA), and GAPDH mouse antibody (1/1000 dilution; Cell signaling Tecnology, USA) overnight at 4°C, washed three times with TBST, and then incubated with horseradish peroxidase-conjugated secondary antibody for 1 hour at 37°C. The blots were imaged using AlphaView system (Cell Biosciences, Santa Clara, CA, USA) and quantified using the Image J 1.48 software (National Institutes of Health).

Zeng N., Xu J., Yao W., Li S., Ruan W, & Xiao F. (2017). Brain-Derived Neurotrophic Factor Attenuates Septic Myocardial Dysfunction via eNOS/NO Pathway in Rats. Oxidative Medicine and Cellular Longevity, 2017, 1721434.

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Quantitative Western Blot Analysis

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Western blotting analyses were performed as previously described9 using 50 μg protein samples from fresh tissues and cells. Primary antibodies included SALL4 (1:500 dilution; sc‐101147; Santa Cruz), GSK3β (1:1000 dilution; sc‐53931; Santa Cruz), β‐catenin (1:1000 dilution; sc‐7963; Santa Cruz), c‐Myc (1:500 dilution; sc‐40; Santa Cruz), Cycline D1(1:500 dilution; sc‐8396; Santa Cruz) and GAPDH (1:1000 dilution; sc‐47724, Santa Cruz). The relative densities of the western blot bands were quantified using the Alpha View system (Cell Biosciences).

Chen M., Li L, & Zheng P. (2019). SALL4 promotes the tumorigenicity of cervical cancer cells through activation of the Wnt/β‐catenin pathway via CTNNB1. Cancer Science, 110(9), 2794-2805.

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