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Pcdna3.1 myc his c expression vector

Manufactured by Thermo Fisher Scientific
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The pcDNA3.1 myc-His C expression vector is a plasmid designed for the expression of recombinant proteins in mammalian cells. It contains a cytomegalovirus (CMV) promoter for high-level protein expression, a myc-His tag for detection and purification of the expressed protein, and antibiotic resistance genes for selection in both bacterial and mammalian cells.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Opti mem, by Thermo Fisher Scientific (3 mentions)

Close spelling variants of this product

PcDNA3.1-myc-His expression vector PcDNA3.1/Myc-His vector PcDNA3.1 myc-His C PcDNA3.1/myc-His PcDNA3.1 (His) vector PcDNA3.1(+)-Myc vector PcDNA3.1/His C PcDNA3.1 expression vector PcDNA3.1(+) (pcDNA3.1) expression vector PcDNA3 expression vector

3 protocols using pcdna3.1 myc his c expression vector

1

Overexpression of CRBP1 in HCT116 Cells

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Full-length CRBP1 cDNA was synthesised from total RNA extracted from HCT116 cells by PCR using Platinum Taq Polymerase and Oligo (dT) primers, and was cloned into the pcDNA3.1 myc-His C expression vector (Invitrogen) using the restriction enzymes EcoRI and XhoI, and the T4 Ligase. The plasmid sequence was directly sequenced to confirm the fidelity of the CRBP1 insert. Cells were transfected with this plasmid using Lipofectamine 2000 (Invitrogen) in Opti-MEM (Invitrogen) as per the manufacturer's instructions.
Yokoi K., Yamashita K., Ishii S., Tanaka T., Nishizawa N., Tsutsui A., Miura H., Katoh H., Yamanashi T., Naito M., Sato T., Nakamura T, & Watanabe M. (2017). Comprehensive molecular exploration identified promoter DNA methylation of the CRBP1 gene as a determinant of radiation sensitivity in rectal cancer. British Journal of Cancer, 116(8), 1046-1056.
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2

Expressing CDO1 in HCT116 Cells

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A full-length cDNA for CDO1 was isolated and cloned into the pcDNA3.1 myc-His C expression vector (Invitrogen). HCT116 cells were transfected with CDO1 plasmid vectors using Lipofectamine 2000 (Invitrogen) in Opti-MEM (Invitrogen) as per the manufacturer’s instructions.
Kojima K., Nakamura T., Ohbu M., Katoh H., Ooizumi Y., Igarashi K., Ishii S., Tanaka T., Yokoi K., Nishizawa N., Yokota K., Kosaka Y., Sato T., Watanabe M, & Yamashita K. (2018). Cysteine dioxygenase type 1 (CDO1) gene promoter methylation during the adenoma-carcinoma sequence in colorectal cancer. PLoS ONE, 13(5), e0194785.
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3

CDO1 cDNA Overexpression Protocol

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Full-length CDO1 cDNA was inserted into the pcDNA3.1 myc-His C expression vector (Invitrogen) as previously described [20 (link)]. Cells were transfected using Lipofectamine 2000 (Invitrogen) in Opti-MEM (Invitrogen).
Harada H., Hosoda K., Moriya H., Mieno H., Ema A., Ushiku H., Washio M., Nishizawa N., Ishii S., Yokota K., Tanaka Y., Kaida T., Soeno T., Kosaka Y., Watanabe M, & Yamashita K. (2019). Cancer-specific promoter DNA methylation of Cysteine dioxygenase type 1 (CDO1) gene as an important prognostic biomarker of gastric cancer. PLoS ONE, 14(4), e0214872.
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