Mice were injected intraperitoneally (IP) with 125 µg of LTA from S. aureus (Sigma-Aldrich, St. Louis, MO, USA) in 50 µL of sterile saline. Lipopolysaccharide (LPS) from Escherichia coli serotype 0127: B8 (Sigma-Aldrich, St. Louis, MO, USA) was injected IP at 125 µg in 50 µL of sterile saline per mouse. Murine recombinant tumor necrosis factor-α (TNFα) was injected IP at 500 ng in 50 µL of sterile phosphate buffered saline per mouse (R&D Systems, Inc., Minneapolis, MN, USA). Control animals were injected IP with 50 µL clinical grade saline (0.9% NaCl). All IP injections were done on the right side. Four hours after IP injections of LPS, LTA, TNFα or saline, animals were prepared for IVM and the microcirculation underlying the parietal peritoneum was observed.
Lipopolysaccharide lps from escherichia coli serotype 0127 b8
Manufactured by Merck Group
Sourced in United States
Lipopolysaccharide (LPS) from Escherichia coli serotype 0127: B8 is a complex molecule found in the outer membrane of Gram-negative bacteria. It is composed of a lipid and a polysaccharide. This product is used for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Lta from s aureus, by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Latex beads, by Thermo Fisher Scientific (1 mentions)
Live dead baclight bacterial viability kit, by Thermo Fisher Scientific (1 mentions)
Necrostatin 1 nec 1, by Merck Group (1 mentions)
Anti tlr4 pe, by Thermo Fisher Scientific (1 mentions)
Electrophoresis reagents, by Bio-Rad (1 mentions)
4 protocols using lipopolysaccharide lps from escherichia coli serotype 0127 b8
1
Murine Inflammatory Responses to LTA, LPS, and TNFα
2
Rat IL-10 and LPS Protocol
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Rat interleukin-10 (IL-10) and lipopolysaccharide (LPS, from Escherichia coli serotype 0127:B8) were purchased from Sigma (St Louis, MO, USA). RPMI 1640 medium were purchased from GIBCO (Grand Island, New York, NY, USA). Penicillin/streptomycin was purchased from Life Technologies (Grand Island, NY, USA).
3
Immune Cell Activation and Inhibition
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Lipopolysaccharide (LPS) from Escherichia coli (serotype 0127:b8) and from S. enterica serotype Typhimurium were purchased from Sigma-Aldrich. Live/Dead BacLight bacterial viability kit and latex beads were obtained from Molecular Probes. Alexa 488-conjugated mAb 205c (13 (link)) was used to label αD for flow cytometry analysis. Anti-TLR4-PE was purchased from eBioscience, San Diego, CA, USA. YVAD (N-Ac–Tyr–Val–Ala–Asp–CMK), a caspase-1 inhibitor, was purchased from Cayman-Chemical. Fluorochrome inhibitor of caspase (FLICA) was purchased from Immunochemistry Technologies (Bloomington, MN, USA). Necrostatin-1 (Nec-1) was obtained from Sigma-Aldrich (Taufkirchen, Germany). Nec-1 is an inhibitor of the receptor interacting protein 1 kinase and acts as an inhibitor of necroptosis (19 (link)).
4
Evaluating Endocrine Disruptors and Pharmaceuticals
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BPA (PubChem CID: 6623), BPAF (PubChem CID: 73864), BPS (PubChem CID: 6626), Mifepristone (RU486) (PubChem CID: 55245), Flutamide (PubChem CID: 3397) and esiRNA MISSION® (EHU068001) for GRα were purchased from Sigma Aldrich (St Louis, MO, United States). Lipopolysaccharide (LPS) from Escherichia coli serotype 0127:B8 and the cell culture media and supplements were from Sigma Aldrich (St Louis, MO, United States). The mouse anti-human RACK1 monoclonal antibody (sc-17754) was from Santa Cruz Biotechnology (Dallas, TX, United States). The mouse monoclonal anti-β-tubulin antibody (T0198) was from Sigma Aldrich (St Louis, MO, United States). Electrophoresis reagents were from Bio-Rad (Richmond, CA, United States). Chemicals were diluted in dimethyl sulfoxide (DMSO) for 50 mM stock solutions, and sequentially properly diluted.
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