Log In Sign up
The largest database of trusted experimental protocols

Mouse anti human igm apc mhm 88

Manufactured by Southern Biotech
Visit Supplier

Mouse anti-human IgM-APC (MHM-88) is a fluorescently-labeled monoclonal antibody that binds to human immunoglobulin M (IgM). The APC (Allophycocyanin) fluorescent label allows for detection and quantification of IgM-expressing cells using flow cytometry or other fluorescence-based applications.

Automatically generated - may contain errors

Lab products found in correlation

Goat anti human igg pe, by Southern Biotech (2 mentions) Mouse anti human cd19 apc, by BioLegend (2 mentions) Mouse anti human cd81 pe, by BioLegend (2 mentions)

2 protocols using mouse anti human igm apc mhm 88

1

Targeted Knockout Validation by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lines were transduced with sgRNA vectors marked by GFP. Three to four days after transduction, GFP levels were measured by flow cytometry on a BD FACS Calibur using CellQuest™ Pro version 6.0 and analyzed with FlowJo version 9. Cells were split every other day into doxycycline containing media and GFP levels were followed for 14 days and normalized to the day 0 measurement. All sgRNA and shRNA sequences are listed below. When surface proteins were targeted, knockout was validated by flow cytometry by spinning cells down, washing in FACS buffer (PBS plus 2% (vol/vol) FBS, 1mM EDTA), and stained at 4°C for 30 minutes in FACS buffer with fluorescently labeled antibodies: mouse anti-human CD19-APC (Biolegend SJ25C1, 1:500), mouse anti-human CD81-PE (Biolegend 5A6, 1:500), mouse anti-human IgM-APC (MHM-88), 1:400); or from Southern Biotech: goat anti-human IgG-PE (1:200).
Phelan J.D., Young R.M., Webster D.E., Roulland S., Wright G.W., Kasbekar M., Shaffer AL I.I.I., Ceribelli M., Wang J.Q., Schmitz R., Nakagawa M., Bachy E., Huang D.W., Ji Y., Chen L., Yang Y., Zhao H., Yu X., Xu W., Palisoc M.M., Valadez R.R., Davies-Hill T., Wilson W.H., Chan W.C., Jaffe E.S., Gascoyne R.D., Campo E., Rosenwald A., Ott G., Delabie J., Rimsza L.M., Rodriguez F.J., Estephan F., Holdhoff M., Kruhlak M.J., Hewitt S.M., Thomas C.J., Pittaluga S., Oellerich T, & Staudt L.M. (2018). A Multiprotein Supercomplex Controlling Oncogenic Signaling in Lymphoma. Nature, 560(7718), 387-391.
+ Open protocol
+ Expand
2

Targeted Knockout Validation by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lines were transduced with sgRNA vectors marked by GFP. Three to four days after transduction, GFP levels were measured by flow cytometry on a BD FACS Calibur using CellQuest™ Pro version 6.0 and analyzed with FlowJo version 9. Cells were split every other day into doxycycline containing media and GFP levels were followed for 14 days and normalized to the day 0 measurement. All sgRNA and shRNA sequences are listed below. When surface proteins were targeted, knockout was validated by flow cytometry by spinning cells down, washing in FACS buffer (PBS plus 2% (vol/vol) FBS, 1mM EDTA), and stained at 4°C for 30 minutes in FACS buffer with fluorescently labeled antibodies: mouse anti-human CD19-APC (Biolegend SJ25C1, 1:500), mouse anti-human CD81-PE (Biolegend 5A6, 1:500), mouse anti-human IgM-APC (MHM-88), 1:400); or from Southern Biotech: goat anti-human IgG-PE (1:200).
Phelan J.D., Young R.M., Webster D.E., Roulland S., Wright G.W., Kasbekar M., Shaffer AL I.I.I., Ceribelli M., Wang J.Q., Schmitz R., Nakagawa M., Bachy E., Huang D.W., Ji Y., Chen L., Yang Y., Zhao H., Yu X., Xu W., Palisoc M.M., Valadez R.R., Davies-Hill T., Wilson W.H., Chan W.C., Jaffe E.S., Gascoyne R.D., Campo E., Rosenwald A., Ott G., Delabie J., Rimsza L.M., Rodriguez F.J., Estephan F., Holdhoff M., Kruhlak M.J., Hewitt S.M., Thomas C.J., Pittaluga S., Oellerich T, & Staudt L.M. (2018). A Multiprotein Supercomplex Controlling Oncogenic Signaling in Lymphoma. Nature, 560(7718), 387-391.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!