The rabbit polyclonal CHCHD4 and rabbit polyclonal BNIP3 antibodies were purchased from Cambridge Biosciences. The mouse monoclonal HIF-1α antibody was purchased from BD Biosciences. The mouse monoclonal β-actin and rabbit polyclonal ATP5B antibodies were purchased from Abcam. The mouse monoclonal anti-myc (9B11) and rabbit monoclonal COXIV (3E11) antibodies were purchased from Cell Signaling Technology. The mouse monoclonal anti-Pimonidazole antibody was purchased from HypoxyProbe, Inc. The donkey anti-rabbit and anti-mouse horseradish peroxidase-linked secondary antibodies were purchased from VWR. Sodium azide was purchased from Sigma-Aldrich and used at 5 mM for 16 h in order to optimally block HIF-1α protein levels without significantly affecting cell viability. DAPI solution (10 mg/mL) was purchased from Cambridge Biosciences. Pimonidazole (200 mM) was purchased from HypoxyProbe, Inc.
Mouse monoclonal anti myc 9b11
Manufactured by Cell Signaling Technology
Mouse monoclonal anti-myc (9B11) is a laboratory reagent produced by Cell Signaling Technology. It is an antibody that specifically binds to the c-Myc protein.
Automatically generated - may contain errors
Lab products found in correlation
Sodium azide, by Merck Group (1 mentions)
β actin mouse monoclonal, by Abcam (1 mentions)
Coxiv 3e11, by Cell Signaling Technology (1 mentions)
Pimonidazole, by Hypoxyprobe (1 mentions)
Alexa fluor 568 conjugated phalloidin, by Thermo Fisher Scientific (1 mentions)
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Mouse monoclonal anti flag m2, by Merck Group (1 mentions)
Pgex 4t 3 vector, by GE Healthcare (1 mentions)
Cnbr activated sepharose 4b beads, by GE Healthcare (1 mentions)
Mouse monoclonal anti puromycin, by Merck Group (1 mentions)
Rabbit monoclonal anti phospho erk, by Cell Signaling Technology (1 mentions)
Mouse monoclonal anti rack1, by BD (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Ecl reagent, by GE Healthcare (1 mentions)
Mouse monoclonal anti β actin, by Merck Group (1 mentions)
3 protocols using mouse monoclonal anti myc 9b11
1
Hypoxia-Induced HIF-1α Signaling Pathway
2
Production and Characterization of KIF13B Antibody
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An anti-KIF13B polyclonal antibody was produced by immunizing rabbits with a purified GST-tagged C-terminal KIF13B protein (1382–1843) expressed in BL21(DE3) Escherichia coli using the pGEX-4T-3 vector (GE Healthcare). The GST tag was removed from the protein by thrombin digestion before immunization. The antiserum was affinity purified using the antigen coupled to CNBr-activated Sepharose 4B beads (GE Healthcare). A rabbit polyclonal anti-KIF5B antibody, generated against the C-terminal sequence of KIF5B, was described previously (Kanai et al., 2000 (link)). Goat polyclonal anti-albumin (Bethyl Laboratories, Inc.), rat monoclonal anti-LAMP2 (ABL-93; Santa Cruz Biotechnology, Inc.), rabbit monoclonal anti-LRP1 (EPR3724; Abcam), rabbit monoclonal anti-LDLr (EP1553Y; Abcam), rabbit polyclonal anti-caveolin (sc-894; Santa Cruz Biotechnology, Inc.), mouse monoclonal anti-clathrin heavy chain (clone 23; BD), rabbit polyclonal anti-hDLG1 (sc-25661; Santa Cruz Biotechnology, Inc.), mouse monoclonal anti-utrophin (20C5, Abcam; 8A4, Santa Cruz Biotechnology, Inc.), mouse monoclonal anti-myc (9B11; Cell Signaling Technology), and mouse monoclonal anti-FLAG (M2; Sigma-Aldrich) primary antibodies were purchased commercially. Alexa Fluor–conjugated secondary antibodies and Alexa Fluor 568–conjugated phalloidin were purchased from Invitrogen.
3
Immunoblotting for Protein Analysis
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Immunoblotting was performed on protein extracts obtained with lysis buffer as previously seen for histidine pull-down. Equal amounts of proteins were loaded on a 10% SDS-PAGE and transferred to the PVDF membrane (Millipore). After blocking in 5% Bovine Serum Albumin in PBS1X for 30 min at 37 °C, blots were incubated with the following primary antibodies: mouse monoclonal anti-RACK1 (BD Biosciences, 1:2000), mouse monoclonal anti-β-actin (Sigma, 1:1000), mouse monoclonal anti-puromycin (Millipore, 1:10,000), mouse monoclonal anti-Myc 9B11 (Cell Signaling, 1:1000), rabbit monoclonal anti-FMRP (Abcam, 1:1000), rabbit polyclonal anti-phospho-FMRP (Abcam, 1:500), rabbit monoclonal anti-phospho-ERK (Cell Signaling, 1:1000), rabbit monoclonal anti-phospho-rpS6 (Cell Signaling, 1:1000). Secondary HRP-conjugated anti-mouse or anti-rabbit antibodies and ECL reagent (1:5000, GE Healthcare) were used. For RACK1, mouse HRP-conjugated anti-IgM (1:5000, Sigma) was used.
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