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3 protocols using pyridine 2 carboxaldehyde

1

Antimicrobial Screening of Hydrazone Derivatives

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1-Adamantyl methyl ketone (99%), pyridine-2-carboxaldehyde (99%), potassium hydroxide (90%), 2,4-dinitrophenylhydrazine (97%), phenylhydrazine hydrochloride (99%), 2-chlorophenyl hydrazine hydrochloride (97%), 4-methoxy-phenyl hydrazine hydrochloride (98%), o-tolyl hydrazine hydrochloride (97%), concentrated sulfuric acid, glacial acetic acid, sodium acetate, ethyl acetate, hexane, methanol, and ethanol were obtained from Sigma–Aldrich.
Gram-negative bacteria species Pseudomonas aeruginosa (ATCC/27853), Klebsiella pneumonia, Salmonella typhimurium and Escherichia coli (ATCC/25922), and Gram-positive bacteria species Bacillus subtilis and Staphylococcus aureus (ATCC/25923) were obtained from Agricultural Biological Laboratory. Gram-negative bacteria (Meropenem, Gentamycin) and Gram-positive bacteria antibiotics (Meropenem, Ampicillin) were used as antibiotic standards, normal saline 0.9%, Mueller–Hinton agar media (MHA), Potato dextrose agar (PDA), chloramphenicol (99%), Fusarium oxysporum fungus were obtained from Agricultural Laboratory.
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2

Polymer Synthesis Protocol

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For polymer synthesis, N,N-(diethylamino)ethyl methacrylate (DEAEM), polyvinylpyrrolidone (MW = 40,000 Da), and pyridine-2-carboxaldehyde were purchased from Sigma Aldrich (St. Louis, MO, USA). Alpha-bromoisobutyryl bromide, basic alumina powder, copper acetate monohydrate, diethyl ether, magnesium sulfate, methylene chloride, n-propylamine, pentane, Pluronic® F127, Pluronic® P123, sea sand, sodium borohydride, triethylamine, and toluene were purchased from Fisher Scientific (Waltham, MA, USA).
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3

Synthesis of Pyridine-based Compounds

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Pyridine-2-carboxaldehyde, 6-methylPyridine-2-carboxaldehyde, 2-(aminomethyl)pyridine, nickel(ii) perchlorate hexahydrate, nitrilotriacetic acid, iodoethane (Sigma-Aldrich), sodium triacetoxyborohydride, 1,2-diaminobenzene, 3-hydroxyflavone (Alfa Aesar), ethyl acetate, triethylamine, sodium sulphate (Merck, India), were used as received. Dichloromethane, diethylether, tetrahydrofuran, acetonitrile (Merck, India) and methanol, acetone (Sisco Research Laboratory, Mumbai) were distilled before use. The supporting electrolyte tetra-N-butylammonium perchlorate (Aldrich) was recrystallized and used.
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