Lncap cells

HEK293T cells, LNCaP cells, MCF7 cells, and A549 cells were purchased from Korea Cell Line Bank (KCLB, Daejeon, Korea). HEK293T cells were cultured in Dulbecco’s modified Eagle’s Medium (DMEM), and LNCaP cells, MCF7 cells, and A549 cells were cultured in Roswell Park Memorial Institute 1640 (RPMI 1640) containing 10% fetal bovine serum, 100 units/mL penicillin, and 100 μg/mL streptomycin (GenDEPOT, Katy, TX, USA). All the cells were maintained at 37 °C in 5% CO₂. For plasmid transfection, 2 M CaCl₂ and 2X HBS buffer (50 mM HEPES, 10 mM KCl, 12 mM glucose, 280 mM NaCl, 1.5 mM Na₂HPO₄, pH 7.05) were used in HEK293T cells, and Effectene (Qiagen, Hilden, Germany) was used in LNCaP cells following the manufacturer’s instructions. For siRNA transfection, Lipofectamine^TM 3000 (Invitrogen, Waltham, MA, USA) was used following the manufacturer’s instructions.

PC3 cells were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). DU145 and LNCaP cells were obtained from the Korean Cell Line Bank (Seoul, South Korea). PC3 and DU145 cells were cultured in Dulbecco’s Modified Eagle’s medium/high glucose (DMEM/high, Cytiva, Marlborough, MA, US), supplemented with 10% heat-inactivated fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA), 1% l-glutamine (Invitrogen), and 1% penicillin and streptomycin (P/S; Invitrogen). LNCaP cells were cultured in Roswell Park Memorial Institute 1640 (RPMI 1640, Invitrogen) supplemented with 10% FBS and 1% P/S. Human bone marrow-derived mesenchymal stem cells (BM-MSCs; Lonza, Basel, Switzerland) were cultured in Mesenchymal Stem Cell Growth Medium (Lonza). For the subculture of BM-MSCs, they were detached with TrypLE (Invitrogen). For all experiments, BM-MSCs between passages 5 and 7 were used. DMEM (Cytiva) supplemented with 10% FBS, 1% l-glutamine, and 1% P/S was used for migration experiments.

LNCaP cells were purchased from the Korean Cell Line Bank (Seoul, Republic of Korea, KCLB numbers: 21740). The cells were cultured in RPMI supplemented with 100 mg/ml penicillin/streptomycin and 10% FBS. They were maintained in a CO₂ incubator at 37°C.