The mixtures of 1 ml of S. mutans (Sm) and S. sanguis (Ss) (3×108 CFU/ml) and 4.0 ml of BHI broth were incubated under microaerophilic conditions for 48 h at 37°C. Then, the culture was centrifuged for 15 min at 2100 rpm to remove all cells. After centrifugation, the supernatant was filtered through 0.22-μm cellulose nitrate filters (Millipore, USA) and stored at 4°C [9 ].
0.22 μm cellulose nitrate filters
Manufactured by Merck Group
Sourced in United States
The 0.22-μm cellulose nitrate filters are a type of laboratory equipment used for filtration purposes. The filters have a pore size of 0.22 micrometers, which allows them to effectively remove small particles, microorganisms, and other contaminants from liquids or gases. These filters are commonly used in various scientific and industrial applications that require highly purified samples or media.
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Lab products found in correlation
2 protocols using 0.22 μm cellulose nitrate filters
1
Cultivation and Harvesting of Oral Streptococci
2
Measuring Selenium Nanoparticle Uptake in Rice
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To investigate SeNPs uptake, 2.5 L portions of the test solution (pH 5.5) containing 2 mM MES and 10 μM SeNPs with or without the rice seedlings (30-day-old) was used. Two milliliter aliquots of the culture solution were obtained at 0, 1, 5, 12, 24, 48, 72, and 120 h during the cultivation period. Subsequently, the collected solutions were filtered through 0.22-μm cellulose nitrate filters (Millipore, Billerica, MA, USA), and stored in the centrifuge tubes at –80 °C for subsequent Se species analysis. Moreover, the rice seedlings were also sampled at the corresponding time set as above to determine the Se content in shoots and roots. Each treatment was replicated three times (two plants per pot).
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