The following Abs were used for ChIP: α-macroH2A1.2 (Millipore MABE61), α-macroH2A1 (Millipore 07–219), α-phospho-H2AX (Millipore 05–636), α-H2AX (Abcam ab20669), α-H2AZ (Abcam ab4174), α-H2B (Abcam ab52484), normal mouse IgG (Millipore 12–371). Antibodies for IP were α-Flag M2 (Sigma F1804) and α-BRCA1 (Santa Cruz sc-6954). Primary antibodies for western blotting were: α-macroH2A1.2 (Millipore MABE61), α-phospho-H2AX (S139) (Millipore 05–636), α-BRCA1 (Santa Cruz sc-6954), α-PCNA (Santa Cruz sc-56), α-GAPDH (Santa Cruz sc-32233), α-SPT16 (Santa Cruz sc-28734), α-EZH2 (CST 5246s), α-SSRP1 (CST 134215), α-phospho-ATM (CST 4526s), α-phospho-CHK2 (CST 2661s), α-phospho-RPA S4/8 (Bethyl A300-245A), α-RPA2 (Millipore NA19L), α-H2AX (Abcam ab20669), α-H2AZ (Abcam ab4174), α-H2A (CST 12349s, Abcam ab15653). Secondary antibodies were goat anti-mouse IgG-HRP (Santa Cruz sc-2005, Invitrogen 31430) and goat anti-rabbit IgG-HRP (Santa Cruz sc-2004, Invitrogen 31460, CST 7074). Primary antibodies for IF were: α-γ-H2AX (Abcam ab11174, Milipore 05–636), α-BRCA1 (Santa Cruz, sc-6954), α-macroH2A1.2 (Millipore, MABE61), α-pRPA S33 (Bethyl, A300-246A), α-FANCD2 (Novus NB100-182). Secondary antibodies were from Life Technologies (goat-α-mouse or goat-α-rabbit conjugated to Alexa Fluor 488, 568, or 647).
Kim J., Sturgill D., Sebastian R., Khurana S., Tran A.D., Edwards G.B., Kruswick A., Burkett S., Hosogane E.K., Hannon W.W., Weyemi U., Bonner W.M., Luger K, & Oberdoerffer P. (2017). Replication stress shapes a protective chromatin environment across fragile genomic regions. Molecular cell, 69(1), 36-47.e7.
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