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Donkey anti chicken igy igg alexa488 703 545 155

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Donkey anti-chicken IgY (IgG) Alexa488 is a secondary antibody conjugated with the Alexa Fluor 488 fluorophore. It is designed to detect and label chicken IgY (IgG) in immunoassays and other applications.

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Lab products found in correlation

Ab92513, by Abcam (2 mentions) Leica sp8 microscope, by Leica camera (2 mentions) Donkey anti goat igg cy3 705 166 147, by Jackson ImmunoResearch (2 mentions) Donkey anti rabbit igg alexa647 a31573, by Thermo Fisher Scientific (2 mentions) Ab13970, by Abcam (2 mentions)

2 protocols using donkey anti chicken igy igg alexa488 703 545 155

1

Tamoxifen-induced Vascular Remodeling in Mice

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Mice were injected with 50 ug tamoxifen at P4 and sacrificed at P9. Both males and females were included in the analysis. Thoracic aortae were excised and fixed in 4% PFA for 2 hours at RT. Whole mount staining was performed with antibodies against CD31, GFP and ERG1 and samples were flat mounted and imaged using a SP8 Leica microscope.
Antibodies: goat anti-mouse CD31 (AF3628, R&D Systems 1:500); rabbit anti-ERG (Clone: EPR3864, ab92513, Abcam, 1:500); chicken anti-GFP (ab13970, Abcam, 1:1000);
Secondary antibodies used were: Donkey anti-goat IgG Cy3 (705-166-147, JacksonImmunoResearch, 1:400), Donkey anti-chicken IgY (IgG) Alexa488 (703-545-155, JacksonImmunoResearch, 1:400), Donkey anti-rabbit IgG Alexa647 (A31573, Life technologies, 1:500).
Sugden W.W., Meissner R., Aegerter-Wilmsen T., Tsaryk R., Leonard E.V., Bussmann J., Hamm M.J., Herzog W., Jin Y., Jakobsson L., Denz C, & Siekmann A.F. (2017). Endoglin controls blood vessel diameter through endothelial cell shape changes in response to haemodynamic cues. Nature cell biology, 19(6), 653-665.
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2

Tamoxifen-induced Vascular Remodeling in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were injected with 50 ug tamoxifen at P4 and sacrificed at P9. Both males and females were included in the analysis. Thoracic aortae were excised and fixed in 4% PFA for 2 hours at RT. Whole mount staining was performed with antibodies against CD31, GFP and ERG1 and samples were flat mounted and imaged using a SP8 Leica microscope.
Antibodies: goat anti-mouse CD31 (AF3628, R&D Systems 1:500); rabbit anti-ERG (Clone: EPR3864, ab92513, Abcam, 1:500); chicken anti-GFP (ab13970, Abcam, 1:1000);
Secondary antibodies used were: Donkey anti-goat IgG Cy3 (705-166-147, JacksonImmunoResearch, 1:400), Donkey anti-chicken IgY (IgG) Alexa488 (703-545-155, JacksonImmunoResearch, 1:400), Donkey anti-rabbit IgG Alexa647 (A31573, Life technologies, 1:500).
Sugden W.W., Meissner R., Aegerter-Wilmsen T., Tsaryk R., Leonard E.V., Bussmann J., Hamm M.J., Herzog W., Jin Y., Jakobsson L., Denz C, & Siekmann A.F. (2017). Endoglin controls blood vessel diameter through endothelial cell shape changes in response to haemodynamic cues. Nature cell biology, 19(6), 653-665.
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