108 female rats of 13 days-old were randomized into four groups: the control group (G1), the treatment with Hesperetin group (G2), the treatment with sodium selenite group (G3), and the treatment with Hesperetin and sodium selenite group (G4). Hesperetin (Wako Pure Chemical Industries Ltd.) was dissolved in 7% ethanol (Wako Pure Chemical Industries Ltd.) and 93% olive oil (Wako Pure Chemical Industries Ltd.). The Hesperetin solution was administered to the G2 and G4 groups (0.4 μg/g bodyweight per day), while solvent alone was given to the G1 and G3 groups. Sodium selenite (Na2SeO3; 20 μmol/g bodyweight; Wako Pure Chemical Industries Ltd) was dissolved in PBS (1X; 130 mM NaCl, 3 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4) and administered to rats in the G2 and G4 groups, while rats in the G1 and G3 groups received PBS alone. Sodium selenite was injected subcutaneously into 13-day-old rats (day 0) 4 h after Hesperetin or solvent was administered. A subcutaneous injection of Hesperetin was given on days 0, 1, and 2. On day 6 (19 days old), the rat lenses were observed with slit-lamp microscopy (Topcon Corp., Tokyo, Japan), and the rats were euthanized. Enucleated eyes were analyzed for degradation of filensin, and the GSH and AsA levels were determined.
Olive oil
Manufactured by Fujifilm
Sourced in Japan
Olive oil is a liquid fat obtained from olives, the fruit of the olive tree. It is commonly used in cooking, cosmetics, pharmaceuticals, and as a fuel for traditional oil lamps.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Fujifilm (2 mentions)
Hesperetin, by Fujifilm (1 mentions)
Ethanol, by Fujifilm (1 mentions)
Slit lamp microscopy, by Topcon (1 mentions)
Bovine gall powder, by Fujifilm (1 mentions)
Lecithin, by Fujifilm (1 mentions)
Sodium taurocholate, by Fujifilm (1 mentions)
Oleic acid, by Fujifilm (1 mentions)
Lipase, by Merck Group (1 mentions)
Epigallocatechin gallate hydrate, by Tokyo Chemical Industry (1 mentions)
Triolein, by Fujifilm (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Acetone, by Fujifilm (1 mentions)
Chremophor el, by Merck Group (1 mentions)
Nitrofen, by Merck Group (1 mentions)
Xanthine oxidase, by Merck Group (1 mentions)
Diethylenetriamine n n n n n pentaacetic acid dtpa, by Dojindo Laboratories (1 mentions)
Cremophor el, by Merck Group (1 mentions)
Skimmed milk, by Fujifilm (1 mentions)
Mouse anti cpt1a antibody, by Abcam (1 mentions)
19 protocols using olive oil
1
Hesperetin and Sodium Selenite Effects on Rat Lens
2
Investigating Lipid Metabolism Modulation
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Olive oil, bovine gall powder, triolein, lecithin (egg yolk), sodium taurocholate, catechin mixture, and oleic acid were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Lipase, glyceride monooleate, and cholesterol were purchased from Sigma-Aldrich Corporation (Tokyo, Japan). Epigallocatechin gallate hydrate (EGCG) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). oleic acid-glyceryl monooleate mixture was purchased from MP Biomedicals (Tokyo, Japan). Indigestible dextrin was purchased from Matsutani Chemical Industry Co., Ltd. (Hyogo, Japan). Pectin was purchased from Sansho Co., Ltd. (Osaka, Japan). IMD was produced by Hayashibara Co., Ltd. (Okayama, Japan)[12 (link)].
3
Liver Injury Model and SHED-Heps Therapy
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A CCl4 solution in olive oil (1.0 mL/kg body weight; CCl4 to olive oil = 1:4 volume/volume; Wako Pure Chemicals, Osaka, Japan) was intraperitoneally injected into C57BL/6J mice twice a week for 8 weeks, as described previously [12 (link), 19 (link)]. Age-matched C57BL/6J mice infused with olive oil (Wako Pure Chemicals) were used as controls. The generated SHED-Heps were washed with sterilized with phosphate-buffered saline and kept in cold phosphate-buffered saline (PBS) on ice and quickly infused into the recipient spleen within 10 min after the preparation to maintain the initially prepared donor cell viability. Four-week-CCl4-treated C57BL/6J mice were intrasplenically infused SHED-Heps (1 × 106/10 g body weight in 100 μL of PBS). Age-matched 4-week-CCl4-treated C57BL/6J mice were infused with PBS (100 μL) as experimental controls. All mice did not receive any immunosuppressants throughout the experiment.
4
CCl4-Induced Liver Injury in Mice
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We injected CCl4 dissolved in olive oil (2 mg/kg; Wako) into 8-week-old mice intraperitoneally and harvested liver samples after 24 h. Prior to the in vivo PI staining, we collected blood samples for measurement of serum AST and ALT.
5
Glutaraldehyde Sensitization Protocol
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Glutaraldehyde (GA, C5H8O2, 50%) was purchased from
Kanto Chemical Co., Inc. (Tokyo, Japan). Acetone and olive oil were purchased from Wako
Pure Chemical Industries (Osaka, Japan). For dermal sensitization, GA was dissolved in
Acetone: olive oil (4:1) to 0.5% (w/v). For inhalation sensitization and challenge, GA was
dissolved in phosphate-buffered saline (PBS). For intratracheal challenge, GA was
dissolved in PBS.
Kanto Chemical Co., Inc. (Tokyo, Japan). Acetone and olive oil were purchased from Wako
Pure Chemical Industries (Osaka, Japan). For dermal sensitization, GA was dissolved in
Acetone: olive oil (4:1) to 0.5% (w/v). For inhalation sensitization and challenge, GA was
dissolved in phosphate-buffered saline (PBS). For intratracheal challenge, GA was
dissolved in PBS.
6
Synthesis of Cyclic Peroxide N-89
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N-89 (Fig. 1 , inset) was produced in our laboratory as follows. First, methoxymethylenecy-clododecane and cyclododecanone were prepared by ozonolysis of a vinyl ether in the presence of hydrogen peroxide in diethyl ether. Next, (cyclododecylidene)bishydroperoxide was produced by combining methoxymethylenecyclododecane and cyclododecanone in the presence of peroxide and ozone in acidic conditions. N-89 was produced from (cyclododecylidene)bishydroperoxide and CsOH·H2O in DMF [8 (link)]. Olive oil was procured from Wako (Osaka, Japan), and Chremophor EL was purchased from Sigma (St. Louis, MO, USA). All other chemicals and reagents were of analytical grade.
7
Nitrofen-Mediated Oxidative Stress Assay
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Nitrofen (2,4-dichlorophenyl-p-nitrophenyl ether; Sigma-Aldrich, St. Louis, MO; 100 mg) was dissolved in 1 ml of olive oil (Wako, Osaka, Japan) for animal experiments and was dissolved in dimethyl sulfoxide (Wako) to 100 mM for further dilution in cell experiments. Diethylenetriamine-N,N,N',N'',N''-pentaacetic acid (DTPA) was from Dojindo (Kumamoto, Japan). FeSO4 and hypoxanthine was from Wako, xanthine oxidase was from Sigma-Aldrich and 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) was from Labotec (Tokyo, Japan). All the chemicals were of analytical grade.
8
Pharmacokinetics of Novel N-251 Compound
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N-251 was synthesized, as described previously [12 (link)]. Cremophor® EL was purchased from Sigma Chemical Co. (St. Louis, MO, USA), and olive oil was obtained from Wako (Osaka, Japan). All other chemicals and reagents were analytical grade commercial products. Various doses of N-251 were administered as solutions to the groups of mice (six in each group) either orally (p.o., as solution in olive oil) or intravenously (i.v., as solution in 10% ethanol, 10% Chremophore® EL, and 80% saline (10:10:80 [v/v/v]).
9
Triglyceride Metabolism and Regulation
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Triglyceride E Test Wako, clofibrate, skimmed milk, olive oil, Dulbecco modified Eagle medium (D-MEM) and fetal calf serum (FCS) were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan). Laemmli sample buffer and polyvinylidene fluoride (PVDF) membrane were obtained from Bio-Rad Laboratories Inc. (Collage Station, AZ, USA). RIPA lysis and extraction buffer, protease and phosphatase inhibitor cocktail and Pierce Western Blotting Substrate Plus were purchased from Thermo Fischer Scientific Inc. (Waltham, MA, USA). Mouse anti-CPT1A antibody was obtained from Abcam (Cambridge, UK). Horse radish peroxidase (HRP)-conjugated goat anti-mouse IgG was purchased from Merck Millipore (Darmstadt, Germany). Anti-β-actin monoclonal antibody was obtained from Sigma-Aldrich (St. Louis, MO, USA). Orlistat (Xenical) was obtained from F. Hoffmann-La Roche, Ltd. (Basel, Switzerland).
10
Liver Injury Model with hDPSC Therapy
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Wild-type mice at 9 weeks of age intraperitoneally received a CCl4 solution twice a week for 4 weeks. The CCl4 solution was freshly prepared CCl4 (0.5 mg/kg in olive oil [Wako Pure Chemicals]; CCl4:olive oil = 1:4 volume/volume). The 4-week-CCl4-treated mice received each donor-derived hDPSC products (0.1 × 106/10 g body weight in PBS) via the spleen [26 (link)]. The hDPSC-transplanted mice were subsequently treated with CCl4. The age-matched mice that received olive oil and sham-operated CCl4 were used as negative and positive controls.
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