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Ultrasensitive mouse insulin elisa kit

Manufactured by Mercodia
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Sourced in Sweden, United States

The Ultrasensitive Mouse Insulin ELISA kit is a laboratory equipment product designed for the quantitative determination of insulin in mouse samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and measure insulin levels.

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Lab products found in correlation

Contour xt, by Bayer (2 mentions) Mouse glucagon elisa kit, by Crystal Chem (2 mentions) Lf 90 instrument, by Bruker (2 mentions) Accu chek, by Roche (2 mentions) Mouse insulin elisa kit, by Mercodia (2 mentions) Ensight, by PerkinElmer (1 mentions) Bio plex pro mouse cytokine 23 plex assay, by Bio-Rad (1 mentions) Colorimetric assay, by Randox (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Mouse cxcl11 duoset elisa kit, by R&D Systems (1 mentions) Cxcl10 quantikine elisa kit, by R&D Systems (1 mentions) Half micro test, by Roche (1 mentions) Ensight multimode plate reader, by PerkinElmer (1 mentions) Contour blood glucose meter, by Bayer (1 mentions) Contour glucometer, by Bayer (1 mentions) Architect c8000 clinical chemistry analyzer, by Abbott (1 mentions) Eglp 35k, by Merck Group (1 mentions) Dpp4 010, by Merck Group (1 mentions) Bio gel p 4 gel, by Bio-Rad (1 mentions) Vitros 5.1 fs chemistry system, by Ortho Clinical Diagnostics (1 mentions)

39 protocols using ultrasensitive mouse insulin elisa kit

1

Ultrasensitive Serum Insulin Quantification

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Insulin concentration in serum after 13 weeks of energy drink/dietary interventions was measured using Ultrasensitive Mouse Insulin ELISA kit (Mercodia, Winston Salem, NC, USA). The procedure conducted was as per the manufacturer’s instructions. Briefly, 25 µL of serum samples were incubated with 100 µL enzyme-conjugated antibody solution for 2 h at room temperature. The samples were subsequently incubated with 200 µL TMB substrate for 15 min, and then the reaction was stopped by adding 50 µL stop solution. Optical density was measured at 450 nm within 30 min after adding the stop solution (EnsightTM, PerkinElmer, Waltham, MA, USA). The results of the standards were used to construct a non-linear log curve, which was then used to interpolate the concentrations of the samples.
Graneri L.T., Mamo J.C., D’Alonzo Z., Lam V, & Takechi R. (2021). Chronic Intake of Energy Drinks and Their Sugar Free Substitution Similarly Promotes Metabolic Syndrome. Nutrients, 13(4), 1202.
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2

Serum Biomarkers Analysis in Mice

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Serum insulin was measured with Ultrasensitive Mouse Insulin ELISA kit (Mercodia, Uppsala, Sweden). d-[6,6-2H2]glucose enrichment in deproteinized plasma was quantified with gas chromatography–mass spectrometry (Agilent Technologies, Waldbronn, Germany) after derivatization of glucose to pentaacetate. Serum TG, cholesterol (Roche/Hitachi, Roche Diagnostics, Mannheim, Germany), and NEFAs (Wako Chemicals GmbH, Neuss, Germany) were assessed photometrically (12 (link)). Cytokines were analyzed in serum and liver homogenates using the Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-Rad Laboratories, Hercules, CA). Hepatic cytokines were expressed per protein content.
Jelenik T., Kaul K., Séquaris G., Flögel U., Phielix E., Kotzka J., Knebel B., Fahlbusch P., Hörbelt T., Lehr S., Reinbeck A.L., Müller-Wieland D., Esposito I., Shulman G.I., Szendroedi J, & Roden M. (2017). Mechanisms of Insulin Resistance in Primary and Secondary Nonalcoholic Fatty Liver. Diabetes, 66(8), 2241-2253.
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3

Plasma Biomarkers for Metabolic Health

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Blood obtained in EDTA-coated syringes was centrifuged at 4°C for 10 min at 4000 rpm. The supernatant plasma was aliquoted and stored at −80°C for further analysis. Non-fasting plasma glucose was measured at an optimised dilution of 1:100 using a commercial colorimetric assay kit (Abcam). Insulin levels were assessed with the Ultrasensitive Mouse Insulin ELISA kit (Mercodia). Finally, plasma triglycerides were measured using a colorimetric assay (Randox). All plasma measures were analysed according to the manufacturer’s instructions. Insulin resistance was calculated using Homeostasis model assessment–insulin resistance (HOMA-IR) with the HOMA Calculator version 2.2.3 (Diabetes Trials Unit), and Triglyceride-glucose (TyG) index according to formula indicated below:
Majimbi M., McLenachan S., Nesbit M., Chen F.K., Lam V., Mamo J, & Takechi R. (2023). In vivo retinal imaging is associated with cognitive decline, blood-brain barrier disruption and neuroinflammation in type 2 diabetic mice. Frontiers in Endocrinology, 14, 1224418.
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4

Insulin Secretion Assay in Pancreatic Cells

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Equal numbers of αTC1-6 and βTC6 cells were cultured in 24-well plates in DMEM (1 g/L glucose) (Gibco) with 10% FBS. To test insulin secretion into the media, cells were incubated with 250 μL low-glucose (1 g/L) and high-glucose (4.5 g/L) serum-free DMEM for 1 h at 37°C. The media were then collected, and insulin content was assessed with an Ultrasensitive Mouse Insulin ELISA kit (Mercodia).
Metzger D.E., Liu C., Ziaie A.S., Naji A, & Zaret K.S. (2014). Grg3/TLE3 and Grg1/TLE1 Induce Monohormonal Pancreatic β-Cells While Repressing α-Cell Functions. Diabetes, 63(5), 1804-1816.
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5

Quantifying Serum Inflammatory Markers

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Serum levels of CXCL9, CXCL10, and CXCL11 were detected using the mouse CXCL9 Quantikine ELISA kit (Cat # MCX900), CXCL10 Quantikine ELISA kit (Cat # MCX100) and the mouse CXCL11 DuoSet ELISA kit (Cat # DY572), all from R&D Systems (Minneapolis, MN) using the protocol provided by the manufacturer. Serum levels of insulin were detected using the Ultrasensitive Mouse Insulin ELISA kit (Cat # 10-1249-01) from Mercodia (Uppsala, Sweden) according to their recommended procedures.
Burke S.J., Karlstad M.D., Eder A.E., Regal K.M., Lu D., Burk D.H, & Collier J.J. (2016). Pancreatic β-cell Production of CXCR3 Ligands Precedes Diabetes Onset. BioFactors (Oxford, England), 42(6), 703-715.
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6

Psilocybin's Impact on Metabolic Biomarkers in Mice

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To assess the circulating level of corticosterone, cholesterol, triglycerides, and insulin, 8-week-old male C57BL/6J mice (double-housed) on a chow diet were randomized to treatment with a single intraperitoneal injection of either 3 mg/kg psilocybin or isotonic saline (n = 8 per group). Blood samples were drawn (from the tail tip) before treatment (0 h) and again, 3 and 24 h following treatment. Samples were immediately centrifuged at 3000 rpm at 4 °C for 10 min (to obtain plasma) and stored at −20 °C for analysis. Blood glucose was measured in a drop of whole blood at each time point using a handheld glucometer (Contour XT, Bayer). Plasma corticosterone was measured at (0, 3, and 24 h) using the Detect X Corticosterone assay kit (Arbor Assays, USA). Plasma cholesterol, triglycerides, and insulin were measured only in 24 h samples using Infinity Cholesterol and Infinity Triglycerides kits (Thermofisher Scientific) and Ultrasensitive Mouse Insulin Elisa Kit (Mercodia).
Fadahunsi N., Lund J., Breum A.W., Mathiesen C.V., Larsen I.B., Knudsen G.M., Klein A.B, & Clemmensen C. (2022). Acute and long-term effects of psilocybin on energy balance and feeding behavior in mice. Translational Psychiatry, 12, 330.
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7

Islet Graft Insulin Content Quantification

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After retrieval, islet bearing kidneys were stored at −80°C until insulin analysis. Graft insulin content was measured, as previously described (43 (link)). Briefly, islet grafts were homogenized and sonicated at 4°C in 10 ml of 2 mmol/L acetic acid containing 0.25% BSA for 2 h. Next, homogenates were sonicated again and centrifuged at 10,000 g for 25min at 4°C, and the supernatant was collected. A total of 5 ml acetic acid was added to the remaining pellets and extracted again by sonication and centrifugation, and the second supernatant was collected. Total volume was measured by a combination of the two supernatants, and the samples were assayed for insulin content using the Ultrasensitive Mouse Insulin Elisa kit (10-1249-01, Mercodia, Sweden).
Yang Z., Li X., Zhang C., Sun N., Guo T., Lin J., Li F, & Zhang J. (2020). Amniotic Membrane Extract Protects Islets From Serum-Deprivation Induced Impairments and Improves Islet Transplantation Outcome. Frontiers in Endocrinology, 11, 587450.
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8

Insulin Secretion Measurement in Islets

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After 48 h of cultivation, islets were collected and assessed for secretion function. Ten islets were preincubated in Krebs-Ringer-bicarbonate buffer (KRBB) containing 2.8 mM glucose for 30 min. After preincubation, islets were incubated in KRBB containing 2.8 mM glucose (basal insulin secretion) for 1 h and in KRBB containing 16.8 mM glucose for 1 h (GSIS) to collect the supernatants. Secreted insulin was determined using the Ultrasensitive Mouse Insulin Elisa kit (10-1249-01, Mercodia, Sweden). The stimulation index was calculated as the ratio of GSIS to basal insulin secretion (39 (link)).
Yang Z., Li X., Zhang C., Sun N., Guo T., Lin J., Li F, & Zhang J. (2020). Amniotic Membrane Extract Protects Islets From Serum-Deprivation Induced Impairments and Improves Islet Transplantation Outcome. Frontiers in Endocrinology, 11, 587450.
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9

Metabolic Assessment of Kaempferol Treatment

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BW and food intake were measured every week through the study. Blood glucose levels were measured biweekly using a glucometer (Kroger, Cincinnati, OH). Body composition of the mice was examined at 0, 4, and 8 weeks after treatment using an LF-90 instrument (Bruker Optics, Inc., Billerica, MA). Four and 6 weeks after treatment with kaempferol, mice fasted for 15 h were adminstered via i.p. injection a single dose of pyruvate (1 g/kg BW), or glucose (1 g/kg BW) for pyruvate and glucose tolerance tests, respectively. Blood glucose levels were then measured at 0, 30, 60, 90, and 180 min and 0, 15, 30, 60, and 120 min after administration of pyruvate or glucose. The area under the curve (AUC) for these tests was calculated using the trapezoidal rule [70 (link)]. At the end of the study, the mice were fasted for 15 h and were then euthanized between 9–11 am. Blood was collected immediately, and various organs were weighed and snap-frozen in liquid nitrogen and then stored at −80°C for further analyses. Plasma lipid profile was analyzed by enzymatic methods using assay kits (Teco Diagnostics, Anaheim, CA). Plasma insulin levels were measured using an ultrasensitive mouse insulin ELISA kit (Mercodia, Inc., Uppsala, Sweden). Plasma glucagon levels were measured using mouse glucagon ELISA kit (Crystal Chem, Downers Grove, IL).
Alkhalidy H., Moore W., Wang Y., Luo J., McMillan R.P., Zhen W., Zhou K, & Liu D. (2018). The Flavonoid Kaempferol Ameliorates Streptozotocin-Induced Diabetes by Suppressing Hepatic Glucose Production. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(9), 2338.
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10

Serum Metabolite Profiling in Mice

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Blood samples were obtained by tail tip bleeding in the morning. Blood glucose was determined using a glucometer (Accutrend; Roche). Serum from blood was obtained by centrifugation of blood at 9391x;g for 15 min at 4°C. The pellet was discarded and serum samples were stored at −80° C for further analysis. Free fatty acid and triglyceride levels were measured in serum using the colorimetric quantification kits Half-micro test (Roche) and Infinity (ThermoScientific), respectively. Insulin was measured in serum with an ultra-sensitive mouse insulin ELISA kit (Mercodia). Corticosterone in serum was measured by ELISA kit (Enzo). All commercial kits were used following the manufacturer’s recommendations.
Marmol P., Krapacher F, & Ibáñez C.F. (2020). Control of brown adipose tissue adaptation to nutrient stress by the activin receptor ALK7. eLife, 9, e54721.
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