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10 μm zdv emitter

Manufactured by Bruker

The 10 μm ZDV emitter is a precision laboratory equipment component designed for analytical applications. It features a 10 micron inner diameter and zero dead volume (ZDV) construction for accurate sample delivery and analysis. The core function of this emitter is to provide controlled sample introduction into analytical instruments.

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2 protocols using 10 μm zdv emitter

1

diaPASEF Analysis by timsTOF SCP

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The analytical column was directly connected to a 10 μm ZDV emitter (Bruker) inside of the Bruker captive source. The capillary voltage was set to 1700 V with the dry gas set at 3.0 L/min and 200° C. Data was acquired by diaPASEF on a Bruker timsTOF SCP with the ion accumulation and trapped ion mobility ramp set to 166 ms. DIA scans were optimized and acquired with 90 m/z windows spanning 300 to 1200 m/z and 0.6 to 1.43 1/K0. A 0.86 s cycle time included one full MS1 scan followed by 4 trapped ion mobility ramps to fragment all ions within the defined region.
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2

diaPASEF-based Proteomic Profiling

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The analytical column was directly connected to a 10 μm ZDV emitter (Bruker) inside of the Bruker captive source. The capillary voltage was set to 1700 V with the dry gas set at 3.0 L/min and 200° C. Data was acquired by diaPASEF on a Bruker timsTOF SCP with the ion accumulation and trapped ion mobility ramp set to 166 ms. DIA scans were optimized and acquired with 90 m/z windows spanning 300 to 1200 m/z and 0.6 to 1.43 1/K0. A 0.86 s cycle time included one full MS1 scan followed by 4 trapped ion mobility ramps to fragment all ions within the defined region.
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