N n dimethylformamide (dmf)

All lipopeptides were synthesized manually using the solid-phase peptide synthesis (SPPS) method and Fmoc chemistry on polystyrene resin modified by a Rink amide linker. Deprotection of the Fmoc groups was carried out in a 20% (v/v) piperidine (Merck, Darmstadt, Germany) solution in DMF (Honeywell, Seelze, Germany) for 15 min at room temperature. Attachment of the protected amino acids was conducted in a DMF/DCM solution (Chempur, Piekary Slaskie, Poland) with coupling agents using a threefold molar excess of DIC (Peptideweb, Zblewo, Poland) and OxymaPure (Iris Biotech GmbH, Marktredwitz, Germany) for 1.5 h at room temperature. All reactions were performed using a Kamush peptide shaker (Kamush, Poland). Every step was preceded by rinsing the resin with DMF and DCM. Chloranil test was used for control of acylation and deprotection processes. The peptides were cleaved from the resin using one of the mixture of TFA, TIS, and water (95:2.5:2.5 v/v). Crude products were precipitated with cold diethyl ether (Chempur, Piekary Slaskie, Poland) and lyophilized.

The reagents employed in this study were of high purity including zinc nitrate hexahydrate (Sigma-Aldrich, USA), imidazole (Sigma-Aldrich, USA), 2-methylimidazol (Sigma-Aldrich, USA), N,N-dimethylformamide (Honeywell, USA), tri-ethyl amine (Sigma-Aldrich, USA), sodium hydroxide (EMSURE^®, USA), lead nitrate (BDH, UK), hydrochloric acid (Fisher Scientific, USA). Ultra-pure water (CORNING Mega Pure^TM, USA), was used for sample preparation and dilution. The glassware used was thoroughly washed, cleaned and dried before each use.
X-ray diffractometer (XRD Rigaku Miniflex-II, Japan) was used to investigate the crystal phases of the synthesized ZIF-60; current was set at 15 mA while the CuKα1 radiation was kept at 40 kV. The 2θ angle range for the analysis was set between 5 to 40° at a scanning rate of 7.00°/min. Thermal stability of ZIF-60 was tested using thermogravimetric analysis (TA Instruments) in N₂ atmosphere using a heating rate of 10 °C/min in the temperature range from 30 to 800 °C. FTIR spectra were collected using Smart iTR NICOLET iS10 FTIR spectroscopy for surface functional groups investigation.
The lead concentration in the aqueous solutions was determined using atomic absorption spectroscopy (PerkinElmer, USA); the samples were passed through 0.2 µm filter (Whatmann, Germany) before subjecting them to analysis. pH measurements were conducted using OAKTON ^® pH 700.

Poly(methylmethacrylate) (M_w = 350.000 g/mol, Aldrich), N,N-dimethylformamide (≥99.8%, Honeywell), N-isopropylacrylamide (97%, Aldrich), N,N′-methylenebisacrylamide (powder, ≥99.5%, Sigma), 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (98%, Adrich), methylene blue hydrate (≥97.0%, Sigma) were used as received. Phosphate buffered saline (PBS, bioreagent for molecular biology, pH 7.4 at 25 °C, Sigma) was prepared by dissolving the content of one PBS packet (NaCl 0.138 M and KCl 0.0027 M) in one liter of water. All solutions were prepared using analytical grade Millipore deionized water.
Poly(ethylene terephthalate) foils having a thickness of 0.038 and 0.006 mm were purchased from Good Fellow. Commercial aluminum foil was used for the electrical contacts.

Dichloromethane (DCM) and N,N-Dimethylformamide (DMF) were purchased from Honeywell Riedel-de Haen™ and were used without further purification. Trifluoroacetic acid (TFA), triisopropylsilane (TIS), 1,2-ethanedithiol (EDT), piperidine and N,N-diisopropylethylamine (DIPEA) were purchased from Merck^®. All Fmoc-L-AA-OH (with the respective side chain protecting group orthogonal to Fmoc-based SPPS) and 2-(1H-Benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) were acquired from Novabiochem™ and Iris Biotech GmbH, respectively.

Lanthanum (III) chloride heptahydrate (LaCl₃·H₂O), terephthalic acid (H₂BDC) were purchased from Sigma Aldrich (MERCK, Darmstadt, Germany). N,N-dimethylformamide was acquire from Honeywell (Warsaw, Poland). Thirty-five percent hydrochloric acid (HCl) was bought from Chempur (Piekary Slaskie, Poland).

Natural graphite flakes (-10 mesh, 99.9%) were supplied by Alfa Aesar, Potsdam, Germany. KMnO₄ (99%), H₂SO₄ (95%), and toluene (≥99.5%) were obtained from Fisher Scientific (Waltham, MA, USA). H₃PO₄ (99%), H₂O₂ (30%), HCl (35 to 38%), and hexane (≥98.5%) were purchased from BDH middle east, Dubai, UAE. Dopamine hydrochloride (DA), ethanol (≥99.8%), tris(hydroxymethyl)aminomethane (Tris), N,N-Dimethylacetamide (DMA, ≥99%), and 1-Methyl-2-pyrrolidinone (NMP, 99.5%) were purchased from Sigma Aldrich (St. Louis, MO, USA). dodecane (≥99%) and N,N-Dimethylformamide (DMF, ≥99.8%) were obtained from Honeywell (Charlotte, NC, USA). Polysulfone substrate (PS-30) with 20 K molecular weight cut-off was purchased from Sepro Membranes, Oceanside, CA, USA. The deionized water (DIW) was produced using the ELGA PURELAB Option water purification system, Lane End, UK. All materials were used as obtained without additional purification.

Macronutrients used were NaNO₃ (PanReac, Barcelona, Spain) and NaH₂PO₄·2H₂O (Honeywell International Inc., Charlotte, NC, USA), while the trace elements used for media preparation were Na₂EDTA (Sigma-Aldrich, St. Louis, MO, USA), FeCl₃·6H₂O (Acros Organics, Geel, Belgium), CuSO₄·5H₂O (Sigma-Aldrich), ZnSO₄·7H₂O (Sigma-Aldrich), CoCl₂·6H₂O (Thermo Fisher Scientific, Pittsburg, PA, USA), MnCl₂·4H₂O (Acros Organics), Na₂MoO₄·2H₂O (Chem-Lab NV, Zedelgem, Belgium). Cyanocobalamin, Thiamine HCl and Biotin were purchased from Sigma-Aldrich.
Chemicals used for analysis were ammonium bicarbonate (Sigma-Aldrich), Folin–Ciocalteu reagent (Sigma-Aldrich), N,N′-dimethylformamide (DMF) (Honeywell), chloroform HPLC grade (Honeywell), mercury (III) oxide red (Sigma-Aldrich), 2,4,6-tri(2–pyridyl)–s–triazine (TPTZ) (Alfa Aesar, Ward Hill, MA, USA), water for analysis (Carlo Erba, Cornaredo, Milan, Italy), methanol HPLC grade (Thermo Fischer Scientific), sodium acetate trihydrate (Merck), acetic acid (PENTA), hydrochloric acid (HCl) (PENTA), 1,1-diphenyl-2-picrylhydrazyl radical (DPPH∙) (Sigma-Aldrich), iron (II) chloride anhydrous (Acros organics), 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium salt hydrate (ferrozine) (Sigma-Aldrich), EDTA-Na₂, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) (Acros organics).